Human sFRP-2 Antibody
Human sFRP-2 Antibody Summary
Leu25-Cys295
Accession # Q96HF1
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human sFRP‑2 by Western Blot. Western blot shows lysates of human adipose tissue and human lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human sFRP-2 Monoclonal Antibody (Catalog # MAB6838) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for sFRP-2 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: sFRP-2
Secreted Frizzled Related Protein-2 (sFRP-2) belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled transmembrane Wnt receptors. The sFRP proteins are approximately 30‑35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C‑terminal domain (1, 2). Mature human sFRP-2, also known as SARP-1, SDF-5, and FRP-2, shares 99% aa sequence identity with mouse and rat sFRP-2 (3). sFRP‑2 is widely expressed during embryogenesis and in the adult in tissues including the eye, heart, lung, colon, intestine, smooth muscle, pancreas, prostate, testis, kidney, brain, teeth and joints, craniofacial mesenchyme, and preadipocytes (3‑6). Depending on the context, sFRP-2 can exert either positive or negative effects on Wnt signaling (7‑9). It also inhibits BMP-induced effects (8, 10). sFRP-2 can be incorporated into the extracellular matrix through interactions with Fibronectin and Integrin alpha 5 beta 1 (11). sFRP-2 plays a variety of roles during tissue morphogenesis including inhibition of the planar cell polarity pathway and myoblast and osteoblast differentiation (8, 10, 12, 13). sFRP-2 is also expressed in multiple myeloma and glioma in which it promotes tumorigenicity (10, 14). At physiological concentrations sFRP-2 enhances BMP-1 mediated proteolysis of Pro-Collagen I, whereas at higher concentrations it inhibits BMP-1 activity (15, 16). This difference is significant considering that sFRP-2 is up‑regulated in fibrotic areas of the heart following myocardial infarction (15, 16). Elevated levels of sFRP-2 then promote the recovery of cardiac function by reducing collagen deposition, remodeling, and calcification and by promoting the engraftment of mesenchymal stem cells into the heart (8, 15).
- Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
- van Amerongen, R. and R. Nusse (2009) Development 136:3205.
- Hu, E. et al. (1998) Biochem. Biophys. Res. Commun. 247:287.
- Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. 94:2859.
- Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636.
- Leimeister, C. et al. (1998) Mech. Dev. 75:29.
- Galli, L.M. et al. (2006) Dev. Dyn. 235:681.
- Alfaro, M.P. et al. (2010) J. Biol. Chem. 285:35645.
- von Marschall, Z. and L.W. Fisher (2010) Biochem. Biophys. Res. Commun. 400:299.
- Oshima, T. et al. (2005) Blood 106:3160.
- Lee, J.-L. et al. (2004) J. Biol. Chem. 279:14602.
- Sugiyama, Y. et al. (2010) Dev. Biol. 338:193.
- Descamps, S. et al. (2008) Cell Tissue Res. 332:299.
- Roth, W. et al. (2000) Oncogene 19:4210.
- He, W. et al. (2010) Proc. Natl. Acad. Sci. 107:21110.
- Kobayashi, K. et al. (2009) Nat. Cell Biol. 11:46.
Product Datasheets
Citation for Human sFRP-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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sFRP2 in the aged microenvironment drives melanoma metastasis and therapy resistance
Authors: A Kaur, MR Webster, K Marchbank, R Behera, A Ndoye, CH Kugel, VM Dang, J Appleton, MP O'Connell, P Cheng, AA Valiga, R Morissette, NB McDonnell, L Ferrucci, AV Kossenkov, K Meeth, HY Tang, X Yin, WH Wood, E Lehrmann, KG Becker, KT Flaherty, DT Frederick
Nature, 2016;532(7598):250-4.
Species: Human
Sample Types: Serum
Applications: ELISA Development (Detection)
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