Human Sin1/MAPKAP1 Antibody

Catalog # Availability Size / Price Qty
MAB8168
MAB8168-SP
Human Sin1/MAPKAP1 Antibody in Western Blot
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human Sin1/MAPKAP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Sin1/MAPKAP1 in ELISA.
Source
Monoclonal Mouse IgG2A Clone # 752219
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Sin1/MAPKAP1
Lys408-Gln522
Accession # Q9BPZ7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Sin1/MAPKAP1 by Western Blot. View Larger

Detection of Human Sin1/MAPKAP1 by Western Blot. Western blot shows lysates of human heart tissue and human kidney tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Sin1/MAPKAP1 Monoclonal Antibody (Catalog # MAB8168) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Sin1/MAPKAP1 at approximately 59-63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sin1/MAPKAP1

MAPKAP1 (mitogen-activated protein kinase (MAPK) associated protein-1), also called Mip1 (MAPK interacting protein 1) or Sin1 (stress-activated protein kinase (SAPK) interacting 1), is a widely expressed 59-65 kDa cytoplasmic phosphoprotein. MAPKAP1 binds to inactive forms of signaling molecules such as MEKK2 and Ras. Alternate mRNA splicing potentially creates isoforms of 323, 330, 372, 475, 486 and 522 amino acids (aa) in humans. The human MAPKAP1 region used as an immunogen is included in all except the smallest isoform, and shares 96% aa sequence identity with mouse and rat MAPKAP1.

Long Name
Mitogen-Activated Protein Kinase Associated Protein 1
Entrez Gene IDs
79109 (Human); 227743 (Mouse); 296648 (Rat)
Alternate Names
JC310; MAPKAP1; MEKK2-interacting protein 1; MGC2745; MIP1; MIP1SIN1SIN1b; Mitogen-activated protein kinase 2-associated protein 1; mitogen-activated protein kinase associated protein 1; mSIN1; ras inhibitor MGC2745; SAPK-interacting protein 1; Sin1; SIN1g; stress-activated map kinase interacting protein 1; Stress-activated map kinase-interacting protein 1; stress-activated protein kinase-interacting 1; target of rapamycin complex 2 subunit MAPKAP1; TORC2 subunit MAPKAP1

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Sin1/MAPKAP1 Antibody

There are currently no reviews for this product. Be the first to review Human Sin1/MAPKAP1 Antibody and earn rewards!

Have you used Human Sin1/MAPKAP1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review