Human Sin1/MAPKAP1 Antibody Summary
Accession # Q9BPZ7
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Sin1/MAPKAP1 by Western Blot. Western blot shows lysates of human heart tissue and human kidney tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Sin1/MAPKAP1 Monoclonal Antibody (Catalog # MAB8168) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Sin1/MAPKAP1 at approximately 59-63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
MAPKAP1 (mitogen-activated protein kinase (MAPK) associated protein-1), also called Mip1 (MAPK interacting protein 1) or Sin1 (stress-activated protein kinase (SAPK) interacting 1), is a widely expressed 59-65 kDa cytoplasmic phosphoprotein. MAPKAP1 binds to inactive forms of signaling molecules such as MEKK2 and Ras. Alternate mRNA splicing potentially creates isoforms of 323, 330, 372, 475, 486 and 522 amino acids (aa) in humans. The human MAPKAP1 region used as an immunogen is included in all except the smallest isoform, and shares 96% aa sequence identity with mouse and rat MAPKAP1.
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