Human SLC25A28 Antibody

R&D Systems | Catalog # MAB11774

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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1117503
View all SLC25A28 Primary Antibodies »

Product Specifications

Immunogen

Synthetic Peptide
Accession # Q96A46

Specificity

Detects a synthetic peptide specific for human SLC25A28 around amino acid 250 in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human SLC25A28 Antibody

Detection of Human SLC25A28 by Western Blot.

Western Blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/ml of Mouse Anti-Human SLC25A28 Monoclonal Antibody (Catalog # MAB11774) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SLC25A28 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human SLC25A28 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SLC25A28

Solute carrier 25 member 28 (SLC25A28) is a mitochondrial carrier protein, categorized within the solute carrier family. This protein plays an essential role in mitochondrial iron transport and homeostasis, which is crucial for maintaining oxidative phosphorylation and overall cellular energy metabolism. SLC25A28 is predominantly involved in iron export from mitochondria to the cytosol, facilitating optimal levels of iron-sulfur cluster formation and heme biosynthesis. The dysfunction or dysregulation of SLC25A28 can lead to significant cellular disturbances, including oxidative stress and impaired mitochondrial function, both of which have been linked to neurodegenerative diseases and other metabolic disorders. Moreover, mutations in the SLC25A28 gene have been implicated in diseases like sideroblastic anemia and mitochondrial iron-loading disorders, demonstrating its central role in systemic iron regulation. Given its critical functions in cellular metabolism and iron homeostasis, SLC25A28 has emerged as a promising biomarker for metabolic and mitochondrial pathologies, with potential therapeutic implications in iron-related disease management.

References

  1. Kunji ERS, King MS, Ruprecht JJ, Thangaratnarajah C. The SLC25 Carrier Family: Important Transport Proteins in Mitochondrial Physiology and Pathology. Physiology (Bethesda). 2020 Sep 1;35(5):302-327. doi: 10.1152/physiol.00009.2020. PMID: 32783608; PMCID: PMC7611780.2. 
  2. Guan H, Xiao L, Hao K, Zhang Q, Wu D, Geng Z, Duan B, Dai H, Xu R, Feng X. SLC25A28 Overexpression Promotes Adipogenesis by Reducing ATGL. J Diabetes Res. 2024 May 4;2024:5511454. doi: 10.1155/2024/5511454. PMID: 38736904; PMCID: PMC11088465.

Long Name

solute carrier family 25 member 28

Alternate Names

MFRN2, MRS3/4, MRS4L, NPD016

Entrez Gene IDs

81894 (Human); 246696 (Mouse); 688811 (Rat)

Gene Symbol

SLC25A28

UniProt

Q96A46 (Human)

Additional SLC25A28 Products

Product Documents for Human SLC25A28 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human SLC25A28 Antibody

For research use only

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