Detection of Human SOCS‑2 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human SOCS‑2 Monoclonal Antibody (Catalog # MAB4979) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for SOCS‑2 was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
SOCS-2 (Suppressor of cytokine signaling 2; also CIS-2 and SSI-2) is a 25 kDa member of the SOCS family of proteins. It is an SH2-domain containing cytoplasmic adaptor protein expressed by multiple cell types. It blocks receptor signaling by binding to phosphorylated tyrosines in the cytoplasmic domain of select receptors, including the receptors for leptin, IGF-I and GH. Human SOCS-2 is 198 amino acids (aa) in length. It contains one SH2 domain (aa 48‑156) and a SOCS box (aa 160‑198) that mediates interaction with elongin B or C of the E3 ubiquitin ligase complex. There is one splice variant that shows an Arg-rich eight aa substitution for aa 31‑39. Full-length SOCS-2 is 94% and 96% aa identical to mouse and porcine SOCS-2, respectively.
Suppressor Of Cytokine Signaling 2
Entrez Gene IDs:
8835 (Human); 84607 (Rat)
CIS2; CIS2CIS-2; Cish2; Cytokine-inducible SH2 protein 2; SOCS2; SOCS-2STAT-induced STAT inhibitor 2; SSI2; SSI-2STAT induced STAT inhibitor-2; SSI2STAT-induced STAT inhibitor-2; STATI2; STATI2suppressor of cytokine signaling-2; suppressor of cytokine signaling 2
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