Human SOCS-7/Nck/NAP4 Antibody

Catalog # Availability Size / Price Qty
Detection of Human SOCS‑7/Nck/NAP4 by Western Blot.
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Product Details
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Human SOCS-7/Nck/NAP4 Antibody Summary

Species Reactivity
Detects endogenous human SOCS-7 in Western blots.
Monoclonal Mouse IgG2B Clone # 519106
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human SOCS-7
Accession # O14512
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SOCS‑7/Nck/NAP4 antibody by Western Blot. View Larger

Detection of Human SOCS‑7/Nck/NAP4 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human SOCS-7/Nck/NAP4 Monoclonal Antibody (Catalog # MAB5809) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOCS-7/Nck/NAP4 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOCS-7/Nck/NAP4

Suppressor of cytokine signaling 7 (SOCS-7; also Nck, Ash, and phospholipase C gamma-binding protein and Nck-associated protein 4) is a 63 kDa member of the SOCS family of proteins. It regulates signaling cascades through protein ubiquitination and/or sequestration. Human SOCS-7 is 581 amino acids (aa) in length and contains four poly-Pro regions, a poly-Gly region, a poly-Gln region, an SH2 domain, and a SOCS box domain. A second isoform for SOCS-7 has a deletion of aa 1‑250 found in isoform 1. Human SOCS-7 is 97% aa identical to mouse SOCS-7. SOCS-7 is constitutively expressed in several tissues, most strongly in the testis and brain.

Long Name
Suppressor Of Cytokine Signaling 7
Entrez Gene IDs
30837 (Human); 192157 (Mouse); 287659 (Rat)
Alternate Names
NAP4; NAP-4; NAP4SOCS-7; Nck; Nck, Ash and phospholipase C binding protein; Nck, Ash and phospholipase C gamma-binding protein; NCKAP4; Nck-associated protein 4; SOCS6; SOCS7; SOCS-7; suppressor of cytokine signaling 7

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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