Human SOCS-7/Nck/NAP4 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human SOCS-7 in Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 519106
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human SOCS-7
    His351-Thr581
    Accession # O14512
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human SOCS‑7/Nck/NAP4 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human SOCS‑7/Nck/NAP4 Monoclonal Antibody (Catalog # MAB5809) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOCS‑7/Nck/NAP4 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOCS-7/Nck/NAP4
Suppressor of cytokine signaling 7 (SOCS-7; also Nck, Ash, and phospholipase C gamma-binding protein and Nck-associated protein 4) is a 63 kDa member of the SOCS family of proteins. It regulates signaling cascades through protein ubiquitination and/or sequestration. Human SOCS-7 is 581 amino acids (aa) in length and contains four poly-Pro regions, a poly-Gly region, a poly-Gln region, an SH2 domain, and a SOCS box domain. A second isoform for SOCS-7 has a deletion of aa 1‑250 found in isoform 1. Human SOCS-7 is 97% aa identical to mouse SOCS-7. SOCS-7 is constitutively expressed in several tissues, most strongly in the testis and brain.
  • Long Name:
    Suppressor Of Cytokine Signaling 7
  • Entrez Gene IDs:
    30837 (Human); 192157 (Mouse); 287659 (Rat)
  • Alternate Names:
    NAP4; NAP-4; NAP4SOCS-7; Nck; Nck, Ash and phospholipase C binding protein; Nck, Ash and phospholipase C gamma-binding protein; NCKAP4; Nck-associated protein 4; SOCS6; SOCS7; SOCS-7; suppressor of cytokine signaling 7
Related Research Areas
Isotype Controls
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Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

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Mouse IgG2B Isotype Control

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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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