Human SOCS-7/Nck/NAP4 Antibody

Catalog #: MAB5809 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5809
MAB5809-SP

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Detection of Human SOCS‑7/Nck/NAP4 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOCS-7/Nck/NAP4 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human SOCS-7 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 519106

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human SOCS-7
His351-Thr581
Accession # O14512

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SOCS‑7/Nck/NAP4 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human SOCS-7/Nck/NAP4 Monoclonal Antibody (Catalog # MAB5809) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOCS-7/Nck/NAP4 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOCS-7/Nck/NAP4

Suppressor of cytokine signaling 7 (SOCS-7; also Nck, Ash, and phospholipase C gamma-binding protein and Nck-associated protein 4) is a 63 kDa member of the SOCS family of proteins. It regulates signaling cascades through protein ubiquitination and/or sequestration. Human SOCS-7 is 581 amino acids (aa) in length and contains four poly-Pro regions, a poly-Gly region, a poly-Gln region, an SH2 domain, and a SOCS box domain. A second isoform for SOCS-7 has a deletion of aa 1‑250 found in isoform 1. Human SOCS-7 is 97% aa identical to mouse SOCS-7. SOCS-7 is constitutively expressed in several tissues, most strongly in the testis and brain.

Long Name

Suppressor Of Cytokine Signaling 7

Entrez Gene IDs

30837 (Human); 192157 (Mouse); 287659 (Rat)

Alternate Names

NAP4; NAP-4; NAP4SOCS-7; Nck; Nck, Ash and phospholipase C binding protein; Nck, Ash and phospholipase C gamma-binding protein; NCKAP4; Nck-associated protein 4; SOCS6; SOCS7; SOCS-7; suppressor of cytokine signaling 7