Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Avian, Avian - Chicken, Chicken, Monkey, Primate - Macaca mulatta (Rhesus Macaque), Transgenic Mouse, Xenograft

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SOX9 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SOX9
Met1-Lys151
Accession # P48436

Specificity

Detects human SOX9 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human SOX10 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human SOX9 Antibody

Detection of Human SOX9 antibody by Western Blot.

Detection of Human SOX9 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, KATO-III human gastric carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for SOX9 at approximately 75 kDa (as indicated). GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of SOX9 in U251-MG cells.

SOX9 was detected in immersion fixed U251‑MG human malignant glioblastoma cell line (Positive) and absent in HEL 92.1.7 human erythroleukemic cell line (Negative) using Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) at 1.7 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SOX9 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

SOX9 in BG01V Human Embryonic Stem Cells.

SOX9 was detected in immersion fixed BG01V human embryonic stem cells differentiated into early proximal lung progenitor cells using Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Detection of Human SOX9 antibody by Simple WesternTM.

Detection of Human SOX9 by Simple WesternTM.

Simple Western lane view shows lysates of normal adjacent tissue and Crohns tissue, loaded at 0.2 mg/mL. A specific band was detected for SOX9 at approximately 107 kDa (as indicated) using 20 µg/mL of Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse SOX9 by Western Blot

Detection of Mouse SOX9 by Western Blot

Comparison of E12.5 Sox9IE/IE and Sox9FE/FE embryos based on EGFP intensity and Western blotting.(A) Left panel: white light images of E12.5 Sox9IE/IE and Sox9FE/FE embryos; right panel: same embryos imaged by fluorescence microscopy showed a higher EGFP fluorescence in the Sox9FE/FE embryo compared to the Sox9IE/IE embryo, with EGFP expression in Sox9-specific domains in both. (B) Mean overall EGFP fluorescence of E12.5 Sox9IE/IE (13880.04±1015.55; n = 8) and Sox9FE/FE (84579.48±2822.09; n = 4) embryos analyzed by flow cytometry. Overall EGFP fluorescence was calculated by multiplying the MFI and percentage of EGFP-positive cells. Two-tailed Student's T test, ***p<0.0001. (C) Western blot analysis of E12.5 Sox9IE/IE and Sox9FE/FE embryo lysates, resolved on SDS-polyacrylamide gel electrophoresis (SDS-PAGE) gels and immunoblotted with anti-Sox9 (upper panel) and anti-GFP (middle panel) antibodies. In the upper panel, Sox9-EGFP fusion protein and Sox9-2A (2A - residual 23 amino acids of F2A) was only detected in the Sox9FE/FE embryo lysate, but not in the CD-1 WT or Sox9IE/IE embryo lysates. Middle panel: Sox9-EGFP fusion protein was not detected in the Sox9FE/FE embryos but EGFP was detected in both the Sox9IE/IE and Sox9FE/FE lysates at predicted molecular weight. CD-1 WT lysate served as negative control for EGFP. Bottom panel: immunoblotting with anti-histone antibody showed equal loading. WT – wild-type. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0028885), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse SOX9 by Immunocytochemistry/Immunofluorescence

Detection of Mouse SOX9 by Immunocytochemistry/Immunofluorescence

Dbx1‐expressing progenitors give rise to preBötC neurons and glia. A. Confocal images of Dbx1CreERT2; Rosa26tdTomato mouse preBötC sections immunostained for NeuN. Dbx1‐derived neurons (white arrowhead) expressed tdTomato and were immunoreactive for NeuN. Dbx1‐derived glia (gray arrowhead) expressed tdTomato and were not immunoreactive for NeuN. B. Confocal images of Dbx1CreERT2; Rosa26tdTomato mouse preBötC sections 72 h after injection with AAV‐hSyn‐GFP. Dbx1‐derived non‐neuronal cells extend diffuse fibrils that are closely apposed to the outer surface of microvasculature (white arrows). C, Confocal images of Dbx1CreERT2; Rosa26tdTomato mouse preBötC sections immunostained for Sox9. Glia (gray arrowheads) were immunoreactive for Sox9. Dbx1‐derived neurons (white arrowhead) expressed tdTomato but were not immunoreactive for Sox9. Dbx1‐derived glia expressed tdTomato and were immunoreactive for Sox9 (see “overlay” panel). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28611151), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX9 by Immunocytochemistry/Immunofluorescence

Detection of Human SOX9 by Immunocytochemistry/Immunofluorescence

Feeder-free expansion of SOX9+ BCs. (A) Immunostaining of SOX9+ BCs with anti-P63, anti-KRT5 and anti-SOX9 antibodies. (B) SOX9+ BCs in rugae of 3rd order human airway by anti-SOX9, anti-P63 and anti-CC10 immunostaining. Scale bar, 100 μm. (C) SOX9+ BCs in rugae of 3rd order human airway by anti-KI67 immunostaining. (D) BC colony cultured on feeder-free condition. (E) Karyotyping of cultured BCs. (F) qPCR showing alveolar and bronchial epithelium marker gene expression of human lung sample and SOX9+ BCs in early (P2) and late (P8) passages. n = 3, biological replicates. Error bars, S.E.M. (G) qPCR showing progenitor cell marker (Krt5, P63 and SOX9) gene expression of human lung sample and SOX9+ BCs in early (P2) and late (P8) passages. n = 3, biological replicates. Error bars, S.E.M. (H) Western blotting showing marker gene expression of human lung sample and SOX9+ BCs in early (P2) and late (P8) passages Image collected and cropped by CiteAb from the following publication (https://academic.oup.com/proteincell/article/9/3/267/6760074), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX9 by Immunocytochemistry/Immunofluorescence

Detection of Human SOX9 by Immunocytochemistry/Immunofluorescence

Transplantated SOX9+ BCs regenerate functional human lungin vivo. (A) Left, direct fluorescence image under stereomicroscope showing NOD-SCID mouse lung without (upper panel) or with (lower panel) GFP-labeled SOX9+ BC transplantation. Right, cryo-section and direct fluorescence imaging of transplanted GFP-labeled SOX9+ BCs in lung parenchyma. Scale bar, 100 μm. (B) Immunofluorescence imaging of transplanted GFP-labeled SOX9+ BCs in lung parenchyma with human specific Lamin A+C marker costaining. (C) Fully differentiated GFP+ cells lost SOX9 marker expression (arrowhead indicated). Scale bar, 10 μm. (D) Confocal image with human specific Lamin A+C immunostaining (HuLamin) showing regenerated type I (AQP5+) alveolar cells. No type II (SPC+) cells were observed. (E) Confocal image showing regenerated AEC1 (AQP5+ and HOPX+). AQP5 as a membrane-bound protein distributes on surface of GFP+ cells. Arrowheads indicated the overlay of HOPX with GFP signal in nucleus. Scale bar, 20 μm. (F) qPCR with human specific primers showing alveolar and bronchiolar epithelium marker gene expression in SOX9+ BC transplanted chimeric lung (AEC1: AQP5 and HOPX; AEC2: SPB and LAMP3; bronchiolar cells: SCGB1A1 and MUC1). Biological replicates, n = 3. Error bars, S.E.M. (G) Left, clonogenic BCs isolated from human cervix epithelium obtained by biopsy. Right, transplantation of equal numbers of BCs from lung and cervix indicated different incorporation efficiency Image collected and cropped by CiteAb from the following publication (https://academic.oup.com/proteincell/article/9/3/267/6760074), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX9 by Immunocytochemistry/Immunofluorescence

Detection of Human SOX9 by Immunocytochemistry/Immunofluorescence

Feeder-free expansion of SOX9+ BCs. (A) Immunostaining of SOX9+ BCs with anti-P63, anti-KRT5 and anti-SOX9 antibodies. (B) SOX9+ BCs in rugae of 3rd order human airway by anti-SOX9, anti-P63 and anti-CC10 immunostaining. Scale bar, 100 μm. (C) SOX9+ BCs in rugae of 3rd order human airway by anti-KI67 immunostaining. (D) BC colony cultured on feeder-free condition. (E) Karyotyping of cultured BCs. (F) qPCR showing alveolar and bronchial epithelium marker gene expression of human lung sample and SOX9+ BCs in early (P2) and late (P8) passages. n = 3, biological replicates. Error bars, S.E.M. (G) qPCR showing progenitor cell marker (Krt5, P63 and SOX9) gene expression of human lung sample and SOX9+ BCs in early (P2) and late (P8) passages. n = 3, biological replicates. Error bars, S.E.M. (H) Western blotting showing marker gene expression of human lung sample and SOX9+ BCs in early (P2) and late (P8) passages Image collected and cropped by CiteAb from the following publication (https://academic.oup.com/proteincell/article/9/3/267/6760074), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOX9 by Immunocytochemistry/Immunofluorescence

Detection of Human SOX9 by Immunocytochemistry/Immunofluorescence

Feeder-free expansion of SOX9+ BCs. (A) Immunostaining of SOX9+ BCs with anti-P63, anti-KRT5 and anti-SOX9 antibodies. (B) SOX9+ BCs in rugae of 3rd order human airway by anti-SOX9, anti-P63 and anti-CC10 immunostaining. Scale bar, 100 μm. (C) SOX9+ BCs in rugae of 3rd order human airway by anti-KI67 immunostaining. (D) BC colony cultured on feeder-free condition. (E) Karyotyping of cultured BCs. (F) qPCR showing alveolar and bronchial epithelium marker gene expression of human lung sample and SOX9+ BCs in early (P2) and late (P8) passages. n = 3, biological replicates. Error bars, S.E.M. (G) qPCR showing progenitor cell marker (Krt5, P63 and SOX9) gene expression of human lung sample and SOX9+ BCs in early (P2) and late (P8) passages. n = 3, biological replicates. Error bars, S.E.M. (H) Western blotting showing marker gene expression of human lung sample and SOX9+ BCs in early (P2) and late (P8) passages Image collected and cropped by CiteAb from the following publication (https://academic.oup.com/proteincell/article/9/3/267/6760074), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Human SOX9 Antibody by Immunohistochemistry

Detection of Mouse Human SOX9 Antibody by Immunohistochemistry

Evolution of alveolar epithelial gene expression patterns in the developing mouse lung. Sections of E15.5, 16.5, 17.5 and 18.5 wild-type mouse lungs stained for markers of differentiation. (A) Green, SOX2 (differentiating bronchioles); red, SOX9 (tips); white, LPCAT1 (tip cells from E16.5, then AT2 cells). (B) Green, CEBPA (sub-set of tip cells from E16.5, then AT2 cells); red, pro-SFTPC (embryonic epithelium, stronger from E16.5, later specific to AT2 cells). (C) Green, pro-SFTPC (stronger from E16.5, later specific to AT2 cells); red, LAMP3 (rare tip cells; AT2 cells); magenta, PDPN (tip cells from E16.5, then AT1 cells). (D) Green, LPCAT1 (tip cells from E16.5, then AT2 cells); red, LAMP3 (rare tip cells; AT2 cells); magenta, PDPN (tip cells from E16.5, then AT1 cells). (E) Green, HOPX (stalk cells from E16.5, AT1 cells); red, SOX9 (tip cells); white, E-CAD (epithelial cells). (F) Green, SOX2 (differentiating bronchioles); red, SOX9 (tips); white, HOPX (stalk cells from E16.5, AT1 cells). (G) Green, HOPX (stalk cells from E16.5, AT1 cells); red, LPCAT1 (tip cells from E16.5, then AT2 cells). Arrows, LPCAT1+ HOPX+ cells; arrowheads, LPCAT1+ HOPX− cells. Blue, DAPI (nuclei). Dashed line, edge of lung. Scale bars: 50 μm in A-F, 20 μm in G and insets. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27578791), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Human SOX9 Antibody by Immunohistochemistry

Detection of Mouse Human SOX9 Antibody by Immunohistochemistry

Glucocorticoid signalling is sufficient, but not essential, to specify alveolar fate. (A) Experimental design: Tomato+ E12.5 or 16.5 tip or stalk was grafted into E12.5 host lung and grown with 50 nM Dx throughout culture. (B) Examples of alveolar-fated tip grafts stained for: green, LPCAT1 (alveolar fate); red, Tomato (graft); white, PDPN (basal and AT1 cells). Arrowheads, PDPN+ AT1 cells. (C) Split bar graph showing results from B. Each type of graft was analysed in at least three independent experiments. (D) E12.5 wild-type lungs were grown with or without Dx for up to 6 days; two independent experimental replicates. Note precocious expression of alveolar markers in the presence of Dx. Lungs cultured without Dx do express LPCAT1 from experimental day 5. Green, LPCAT1 (late tip progenitors and type 2 cells); red, SOX9 (tip progenitors). (E,F) Sections of GR−/− and GR+/+ sibling lungs at E17.5 and E18.5 stained for: green, HOPX (AT1 cells); red, SOX9 (tip progenitors); white, E-CAD (epithelium) (E), and: green, LPCAT1 (late tip progenitors and AT2 cells); red, LAMP3 (AT2 cells); magenta, PDPN (late tip progenitors and AT1 cells) (F). A total of five GR−/− and 5 GR+/+ sibling lungs from three independent litters were observed at both E17.5 and E18.5. Blue, DAPI. Dashed line, edge of lung. Scale bars: 100 μm in B; 50 μm in D-F. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27578791), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Human SOX9 Antibody by Immunohistochemistry

Detection of Mouse Human SOX9 Antibody by Immunohistochemistry

Evolution of alveolar epithelial gene expression patterns in the developing mouse lung. Sections of E15.5, 16.5, 17.5 and 18.5 wild-type mouse lungs stained for markers of differentiation. (A) Green, SOX2 (differentiating bronchioles); red, SOX9 (tips); white, LPCAT1 (tip cells from E16.5, then AT2 cells). (B) Green, CEBPA (sub-set of tip cells from E16.5, then AT2 cells); red, pro-SFTPC (embryonic epithelium, stronger from E16.5, later specific to AT2 cells). (C) Green, pro-SFTPC (stronger from E16.5, later specific to AT2 cells); red, LAMP3 (rare tip cells; AT2 cells); magenta, PDPN (tip cells from E16.5, then AT1 cells). (D) Green, LPCAT1 (tip cells from E16.5, then AT2 cells); red, LAMP3 (rare tip cells; AT2 cells); magenta, PDPN (tip cells from E16.5, then AT1 cells). (E) Green, HOPX (stalk cells from E16.5, AT1 cells); red, SOX9 (tip cells); white, E-CAD (epithelial cells). (F) Green, SOX2 (differentiating bronchioles); red, SOX9 (tips); white, HOPX (stalk cells from E16.5, AT1 cells). (G) Green, HOPX (stalk cells from E16.5, AT1 cells); red, LPCAT1 (tip cells from E16.5, then AT2 cells). Arrows, LPCAT1+ HOPX+ cells; arrowheads, LPCAT1+ HOPX− cells. Blue, DAPI (nuclei). Dashed line, edge of lung. Scale bars: 50 μm in A-F, 20 μm in G and insets. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27578791), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Human SOX9 Antibody by Immunohistochemistry

Detection of Mouse Human SOX9 Antibody by Immunohistochemistry

Glucocorticoid signalling is sufficient, but not essential, to specify alveolar fate. (A) Experimental design: Tomato+ E12.5 or 16.5 tip or stalk was grafted into E12.5 host lung and grown with 50 nM Dx throughout culture. (B) Examples of alveolar-fated tip grafts stained for: green, LPCAT1 (alveolar fate); red, Tomato (graft); white, PDPN (basal and AT1 cells). Arrowheads, PDPN+ AT1 cells. (C) Split bar graph showing results from B. Each type of graft was analysed in at least three independent experiments. (D) E12.5 wild-type lungs were grown with or without Dx for up to 6 days; two independent experimental replicates. Note precocious expression of alveolar markers in the presence of Dx. Lungs cultured without Dx do express LPCAT1 from experimental day 5. Green, LPCAT1 (late tip progenitors and type 2 cells); red, SOX9 (tip progenitors). (E,F) Sections of GR−/− and GR+/+ sibling lungs at E17.5 and E18.5 stained for: green, HOPX (AT1 cells); red, SOX9 (tip progenitors); white, E-CAD (epithelium) (E), and: green, LPCAT1 (late tip progenitors and AT2 cells); red, LAMP3 (AT2 cells); magenta, PDPN (late tip progenitors and AT1 cells) (F). A total of five GR−/− and 5 GR+/+ sibling lungs from three independent litters were observed at both E17.5 and E18.5. Blue, DAPI. Dashed line, edge of lung. Scale bars: 100 μm in B; 50 μm in D-F. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27578791), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Human SOX9 Antibody by Immunohistochemistry

Detection of Mouse Human SOX9 Antibody by Immunohistochemistry

Evolution of alveolar epithelial gene expression patterns in the developing mouse lung. Sections of E15.5, 16.5, 17.5 and 18.5 wild-type mouse lungs stained for markers of differentiation. (A) Green, SOX2 (differentiating bronchioles); red, SOX9 (tips); white, LPCAT1 (tip cells from E16.5, then AT2 cells). (B) Green, CEBPA (sub-set of tip cells from E16.5, then AT2 cells); red, pro-SFTPC (embryonic epithelium, stronger from E16.5, later specific to AT2 cells). (C) Green, pro-SFTPC (stronger from E16.5, later specific to AT2 cells); red, LAMP3 (rare tip cells; AT2 cells); magenta, PDPN (tip cells from E16.5, then AT1 cells). (D) Green, LPCAT1 (tip cells from E16.5, then AT2 cells); red, LAMP3 (rare tip cells; AT2 cells); magenta, PDPN (tip cells from E16.5, then AT1 cells). (E) Green, HOPX (stalk cells from E16.5, AT1 cells); red, SOX9 (tip cells); white, E-CAD (epithelial cells). (F) Green, SOX2 (differentiating bronchioles); red, SOX9 (tips); white, HOPX (stalk cells from E16.5, AT1 cells). (G) Green, HOPX (stalk cells from E16.5, AT1 cells); red, LPCAT1 (tip cells from E16.5, then AT2 cells). Arrows, LPCAT1+ HOPX+ cells; arrowheads, LPCAT1+ HOPX− cells. Blue, DAPI (nuclei). Dashed line, edge of lung. Scale bars: 50 μm in A-F, 20 μm in G and insets. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27578791), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SOX9 by Western Blot

Detection of SOX9 by Western Blot

Validation of stem cell associated proteins after HGF stimulation.A, FACS demonstrated up-regulation of CD49b (240%), CD49f (22%), CD44 (26%), together with down-regulation of CD24 (−69%) after HGF stimulation. Thin gray line represents labelling with secondary antibody only (negative control), thin black line DU145 without HGF, and thick black line DU145 with HGF. B, Western blot showed enhancement of SOX9 expression upon HGF stimulation from 2 to 24 hours. C, FACS demonstrated induction of double-labelled CD44+/CD24− DU145 cells (control 8% versus HGF 26%). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22110593), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human SOX9 Antibody

Application
Recommended Usage

Immunocytochemistry

1-15 µg/mL
Sample: Immersion fixed U251-MG human malignant glioblastoma cell line and BG01V human embryonic stem cells differentiated into early proximal lung progenitor cells

Simple Western

20 µg/mL
Sample: Normal adjacent tissue and Crohns tissue

Western Blot

0.5 µg/mL
Sample: KATO‑III human gastric carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and Hep3B human hepatocellular carcinoma cell line

Reviewed Applications

Read 8 reviews rated 4.5 using AF3075 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SOX9

SOX9 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX9 is expressed in mesenchymal progenitors that give rise to chondrocytes and osteoblasts. It is also expressed in the central nervous system, neural crest, intestine, pancreas, and testis. Mutations in SOX9 are associated with defects in sex determination, cartilage and bone development, as well as abnormalities of the heart, kidneys, brain, gut, and pancreas.

Long Name

Transcription Factor SOX9

Alternate Names

campomelic dysplasia, autosomal sex-reversal, CMD 1, CMD1, CMPD1, SRA1SRY (sex-determining region Y)-box 9 protein, SRY (sex determining region Y)-box 9, SRY-related HMG-box, gene 9, transcription factor SOX-9

Entrez Gene IDs

6662 (Human)

Gene Symbol

SOX9

UniProt

P48436

Additional SOX9 Products

Product Documents for Human SOX9 Antibody

Certificate of Analysis

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Product Specific Notices for Human SOX9 Antibody

For research use only

Citations for Human SOX9 Antibody

Customer Reviews for Human SOX9 Antibody (8)

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Showing  1 - 5 of 8 reviews Showing All
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  • Human SOX9 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: BG01V human embryonic stem cells
    Species: Human
    Verified Customer | Posted 12/01/2024
    Human SOX9 Antibody AF3075
  • Human SOX9 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult brain
    Species: Mouse
    Verified Customer | Posted 08/28/2024
    frozen section of adult mouse brain
    Human SOX9 Antibody AF3075
  • Human SOX9 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Embryo tissue
    Species: Mouse
    Verified Customer | Posted 07/14/2020
    Human SOX9 Antibody AF3075
  • Human SOX9 Antibody
    Name: Chang Xie
    Sample Tested: Embryonic lung
    Species: Mouse
    Verified Customer | Posted 03/20/2019
    PFA fixed, sectioned, PBS washed, treated for 5 min in cold Methanol. Worked great!
  • Human SOX9 Antibody
    Name: Paula Aliberti
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human testis
    Species: Human
    Verified Customer | Posted 10/09/2017
    Three month old human testis. Sox9 specifically stained in Sertoli cell nuclei within the seminiferous tubule.
    Human SOX9 Antibody AF3075
  • Human SOX9 Antibody
    Name: Preethi Vijayaraj
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: iPSC differentiated epithelial cells
    Species: Human
    Verified Customer | Posted 09/27/2017
    Human SOX9 Antibody AF3075
  • Human SOX9 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: stem cells
    Species: Human
    Verified Customer | Posted 12/15/2016
    For immunohistochemistry & immunocytochemistry application.
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 22791629
    Species: Rat
    Verified Customer | Posted 01/07/2015

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