Human SOX9 Antibody AF3075: R&D Systems

Human SOX9 Antibody

(16 citations)
(2 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human SOX9 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) SOX10 is observed, and less than 15% rhSOX11, rhSOX12, and rhSOX15 is observed, and less than 5% cross-reactivity with rhSOX1, rhSOX2, rhSOX3, rhSOX5, rhSOX6, rhSOX7, rhSOX8, rhSOX13, rhSOX17, rhSOX18, rhSOX21, and rhSOX30 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human SOX9
    Met1-Lys151
    Accession # P48436
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human SOX9 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, KATO‑III human gastric carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOX9 at approximately 75 kDa (as indicated). GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
SOX9 in HEK293 Human Cell Line. SOX9 was detected in immersion fixed HEK293 human embryonic kidney cell line using 10 µg/mL Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
SOX9 in BG01V Human Embryonic Stem Cells. SOX9 was detected in immersion fixed BG01V human embryonic stem cells differentiated into early proximal lung progenitor cells using Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX9
SOX9 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX9 is expressed in mesenchymal progenitors that give rise to chondrocytes and osteoblasts. It is also expressed in the central nervous system, neural crest, intestine, pancreas, and testis. Mutations in SOX9 are associated with defects in sex determination, cartilage and bone development, as well as abnormalities of the heart, kidneys, brain, gut, and pancreas.
  • Long Name:
    Transcription Factor SOX9
  • Entrez Gene IDs:
    6662 (Human)
  • Alternate Names:
    campomelic dysplasia, autosomal sex-reversal; CMD 1; CMD1; CMPD1; SOX9; SRA1SRY (sex-determining region Y)-box 9 protein; SRY (sex determining region Y)-box 9; SRY-related HMG-box, gene 9; transcription factor SOX-9
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. YAP is essential for mechanical force production and epithelial cell proliferation during lung branching morphogenesis
    Authors: C Lin, E Yao, K Zhang, X Jiang, S Croll, K Thompson-P, PT Chuang
    Elife, 2017;6(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. SOX9 is an astrocyte-specific nuclear marker in the adult brain outside the neurogenic regions
    Authors: W Sun, A Cornwell, J Li, S Peng, MJ Osorio, NA Su Wanga, A Benraiss, N Lou, SA Goldman, M Nedergaard
    J. Neurosci, 2017;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  3. An Integrative Developmental Genomics and Systems Biology Approach to Identify an In Vivo Sox Trio-Mediated Gene Regulatory Network in Murine Embryos
    Authors: WJ Lee, S Chatterjee, SP Yap, SL Lim, X Xing, P Kraus, W Sun, X Hu, V Sivakamasu, HY Chan, PR Kolatkar, S Prabhakar, T Lufkin
    Biomed Res Int, 2017;2017(0):8932583.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ChIP
  4. Fate mapping neurons and glia derived from Dbx1-expressing progenitors in mouse preB�tzinger complex
    Authors: A Kottick, CA Martin, CA Del Negro
    Physiol Rep, 2017;5(11):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Acellular lung scaffolds direct differentiation of endoderm to functional airway epithelial cells: requirement of matrix-bound HS proteoglycans.
    Authors: Shojaie S, Ermini L, Ackerley C, Wang J, Chin S, Yeganeh B, Bilodeau M, Sambi M, Rogers I, Rossant J, Bear C, Post M
    Stem Cell Reports, 2015;4(3):419-30.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Not Specified
  6. Expression of SOX9 and CDX2 in nongoblet columnar-lined esophagus predicts the detection of Barrett's esophagus during follow-up.
    Authors: Zhang X, Westerhoff M, Hart J
    Mod Pathol, 2015;28(5):654-61.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. In vivo genome-wide analysis of multiple tissues identifies gene regulatory networks, novel functions and downstream regulatory genes for Bapx1 and its co-regulation with Sox9 in the mammalian vertebral column.
    Authors: Chatterjee S, Sivakamasundari V, Yap S, Kraus P, Kumar V, Xing X, Lim S, Sng J, Prabhakar S, Lufkin T
    BMC Genomics, 2014;15(0):1072.
    Species: Mouse
    Sample Type: Chromatin
    Application: ChIP
  8. The hippo pathway effector Yap controls patterning and differentiation of airway epithelial progenitors.
    Authors: Mahoney J, Mori M, Szymaniak A, Varelas X, Cardoso W
    Dev Cell, 2014;30(2):137-50.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  9. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
    Authors: Ramasamy S, Kusumbe A, Wang L, Adams R
    Nature, 2014;507(7492):376-80.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  10. Two nested developmental waves demarcate a compartment boundary in the mouse lung.
    Authors: Alanis, Denise M, Chang, Daniel R, Akiyama, Haruhiko, Krasnow, Mark A, Chen, Jichao
    Nat Commun, 2014;5(0):3923.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  11. Identification of SOX3 as an XX male sex reversal gene in mice and humans.
    Authors: Sutton E, Hughes J, White S
    J. Clin. Invest., 2011;121(1):328-41.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  12. The vanishing testis: a histomorphologic and clinical assessment.
    Authors: Antic T, Hyjek EM, Taxy JB
    Am. J. Clin. Pathol., 2011;136(6):872-80.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  13. Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold.
    Authors: Cavallo C, Desando G, Facchini A, Grigolo B
    J Biomed Mater Res A, 2010;93(1):86-95.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  14. Cyclic GMP-dependent protein kinase II inhibits cell proliferation, Sox9 expression and Akt phosphorylation in human glioma cell lines.
    Authors: Swartling FJ, Ferletta M, Kastemar M, Weiss WA, Westermark B
    Oncogene, 2009;28(35):3121-31.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  15. A Novel Approach for the Derivation of Putative Primordial Germ Cells and Sertoli Cells from Human Embryonic Stem Cells.
    Authors: Bucay N, Yebra M, Cirulli V, Afrikanova I, Kaido T, Hayek A, Montgomery AM
    Stem Cells, 2008;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  16. FOXL2 and SOX9 as parameters of female and male gonadal differentiation in patients with various forms of disorders of sex development (DSD).
    Authors: Hersmus R, Kalfa N, de Leeuw B, Stoop H, Oosterhuis JW, de Krijger R, Wolffenbuttel KP, Drop SL, Veitia RA, Fellous M, Jaubert F, Looijenga LH
    J. Pathol., 2008;215(1):31-8.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Expand to show all 16 Citations
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Average Rating: 4 (Based on 2 reviews)

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We have 2 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human SOX9 Antibody AF3075
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Excellent
 ICC/IF Human Anonymous 09/27/2017
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Immunocytochemistry/Immunofluorescence Human SOX9 Antibody AF3075
Immunocytochemistry/Immunofluorescence: Human SOX9 Antibody [AF3075]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestediPSC differentiated epithelial cells
SpeciesHuman
  Average
 ICC/IF Human Anonymous 12/15/2016
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample Testedstem cells
SpeciesHuman

Other Experimental Details

Other Experimental DetailsFor immunohistochemistry & immunocytochemistry application.

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