Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 769653
View all Spi-B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Spi-B
Ala7-Leu115
Accession # Q01892

Specificity

Detects human Spi-B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Spi-B is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Spi‑B Antibody

Detection of Human Spi-B antibody by Western Blot.

Detection of Human Spi‑B by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Spi-B Monoclonal Antibody (Catalog # MAB7576) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Spi-B at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Spi‑B Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Spi-B

Spi-B is a 33-45 kDa member of the ets family of transcription factors. It is found in hematopoietic cells such as B cells and plasmacytoid dendritic cells (DC). In transitional B cells, Spi-B promotes their differentiation into follicular (naïve) B cells. In hematopoietic stem cells, Spi-B stimulates the generation of IFN-producing plasmacytoid DC at the expense of T, B and NK cell development. The 262 amino acid (aa) human Spi-B contains a dual transactivation region (aa 1-62) and an ets DNA-binding domain (aa 169-252). One 177 aa variant diverges after aa 162. Over aa 7-115, human Spi-B shares 72% and 70% aa identity with mouse and rat Spi-B, respectively.

Long Name

Transcription Factor Spi [Spleen FFV Proviral Integration Oncogene] B

Alternate Names

SpiB

Entrez Gene IDs

6689 (Human); 272382 (Mouse); 499146 (Rat)

Gene Symbol

SPIB

UniProt

Q01892 (Human)

Additional Spi-B Products

Product Documents for Human Spi‑B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Spi‑B Antibody

For research use only

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FAQs

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail