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Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 769653
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Product Specifications
Immunogen
E. coli-derived recombinant human Spi-B
Ala7-Leu115
Accession # Q01892
Ala7-Leu115
Accession # Q01892
Specificity
Detects human Spi-B in direct ELISAs.
In direct ELISAs, no cross-reactivity
with recombinant mouse Spi-B is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Spi-B Antibody (769653)
Detection of Human Spi‑B by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Spi-B Monoclonal Antibody (Catalog # MAB7576) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Spi-B at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Spi-B Antibody (769653)
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line
Sample: Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Spi-B
Long Name
Transcription Factor Spi [Spleen FFV Proviral Integration Oncogene] B
Alternate Names
SpiB
Gene Symbol
SPIB
UniProt
Additional Spi-B Products
Product Documents for Human Spi-B Antibody (769653)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Spi-B Antibody (769653)
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
