Human Spinesin Antibody

Catalog # Availability Size / Price Qty
AF2495
AF2495-SP
Spinesin in Human brain (spinal cord).
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Product Details
Citations (1)
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Human Spinesin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Spinesin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 35% cross-reactivity with recombinant mouse Spinesin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Spinesin
Tyr27-Leu413
Accession # Q0P514
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Spinesin (Catalog # 2495‑SE)
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human dorsal root ganglia and human brain (spinal cord).
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Spinesin (Catalog # 2495-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Spinesin in Human brain (spinal cord). Spinesin was detected in immersion fixed paraffin-embedded sections of Human brain (spinal cord) using Goat Anti-Human Spinesin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2495) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to motoneurons in ventral horn in the spinal cord.

Immunohistochemistry View Larger

Spinesin in Human brain. Spinesin was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human Spinesin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2495) at 1.5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (red; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spinesin

Spinesin, encoded by the TMPRSS5 gene, is a member of type II transmembrane serine proteases (TTSPs) (1). Human Spinesin contains the following structural domains: a short N-terminal cytoplasmic tail (amino acid residues 1‑49), a transmembrane domain (residues 50‑70), a stem region and a serine protease domain (residues 71‑457) (2). The domain structure of Spinesin is common to other TTSPs, many of which have additional domains. The stem region of Spinesin contains a scavenger receptor-like domain. The ectodomain of human Spinesin (residues 71‑457) was expressed and purified as a single chain pro-enzyme. The deduced amino acid sequence contains a Leu instead of a Phe residue at position 369; the former is identical to the mouse protein (3, 4). The pro-enzyme can be activated and the resulting enzyme activity can be measured as described in the Activity Assay Protocol.

References
  1. Hooper, J.D. et al. (2001) J. Biol. Chem. 276:857.
  2. Yamaguchi, Y. et al. (2002) J. Biol. Chem. 277:6806.
  3. Carninci, P. et al. (2000) Genome Res. 10:1617.
  4. Shibata, K. et al. (2000) Genome Res. 10:1757.
Entrez Gene IDs
80975 (Human); 80893 (Mouse)
Alternate Names
EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; MGC141886; MGC148044; Spinesin; TMPRSS5; transmembrane protease serine 5; transmembrane protease, serine 5

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Citation for Human Spinesin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Transmembrane protease serine 5: a novel Schwann cell plasma marker for CMT1A
    Authors: Hongge Wang, Matthew Davison, Kathryn Wang, Tai‐He Xia, Martin Kramer, Katherine Call et al.
    Annals of Clinical and Translational Neurology

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