Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 669534
View all SPRY1 Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant human SPRY1
Met1-Lys178
Accession # O43609

Specificity

Detects human SPRY1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human SPRY2, 3, or 4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human SPRY1 Antibody

Detection of Human SPRY1 antibody by Western Blot.

Detection of Human SPRY1 by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line and LNCaP human prostate cancer cell line. PVDF Membrane was probed with 0.5 µg/mL of Human SPRY1 Monoclonal Antibody (Catalog # MAB6097) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SPRY1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human SPRY1 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: 293T human embryonic kidney cell line and LNCaP human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SPRY1

SPRY1 (sprouty homolog 1) is a 34-38 kDa member of the sprouty family of proteins.  It is widely expressed, being found in multiple embryonic and adult tissues.  SPRY1 is considered a negative regulator of cellular signaling.  In particular, it appears to both inhibit MAP kinase signaling following RTK activation, and block TCR signaling following antigen activation.  It interacts with a number of molecules, including PLC-gamma 1, LAT, CBL, caveolin-1 and SPRY2.  Human SPRY1 is 319 amino acids (aa) in length.  It contains one CBL-TKB binding site (aa 51-57) that is phosphorylated at Tyr53, a Ser-rich region (aa 112-131), and a Cys-rich domain (aa 181-306) that mediates intracellular translocation.  SPRY1 undergoes serine phosphorylation, ubiquitination and palmitoylation, the latter which induces SPRY1 to associate with cell membranes.  Over aa 1-178, human SPRY1 shares 76% aa identity with mouse SPRY1.         

Long Name

Sprouty Homolog 1

Alternate Names

Drosophila, homolog of, 1 (antagonist of FGF signaling), sprouty homolog 1, antagonist of FGF signaling (Drosophila), spry-1

Entrez Gene IDs

10252 (Human); 24063 (Mouse); 294981 (Rat)

Gene Symbol

SPRY1

UniProt

O43609 (Human)

Additional SPRY1 Products

Product Documents for Human SPRY1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SPRY1 Antibody

For research use only

Related Research Areas

FGF Family

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