Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 751512
View all SREBP2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SREBP2
Leu242-Asp450
Accession # Q12772

Specificity

Detects human SREBP2 in ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human SREBP2 Antibody

SREBP2 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

SREBP2 in HepG2 Human Cell Line.

SREBP2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human SREBP2 Monoclonal Antibody (Catalog # MAB7119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
SREBP2 antibody in Human Kidney by Immunohistochemistry (IHC-P).

SREBP2 in Human Kidney.

SREBP2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human SREBP2 Monoclonal Antibody (Catalog # MAB7119) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human SREBP2 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Reviewed Applications

Read 1 review rated 5 using MAB7119 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SREBP2

SREBP2 (Sterol Regulatory Element-Binding Protein 2; also bHLHD2 and SREBF2) is a 120-125 kDa member of the SREBP family of proteins. It is ubiquitously expressed and found in the intracellular membrane fraction of cells. SREBP2 is a transcriptional factor initially embedded in the ER as an inactive precursor associated with SCAP. When necessary, SCAP mediates SREBP2 transfer to the Golgi, where two resident proteases remove the N-terminus from SREBP2, and the N-terminus is transported into the nucleus. Here, SREBP2 acts as a transcription factor, activating the LDLR and cholesterol synthesis genes. The human SREBP2 precursor is an 1141 amino acid (aa) two transmembrane protein whose N- and C-termini are cytoplasmic. The two cytoplasmic domains span aa 1-479 and 555-1141, respectively. Proteolytic cleavage between Leu484-Cys485 generates the 64-66 kDa SREBP2 transcription factor. This fragment contains a bHLH DNA binding domain (aa 330-380) and one Leu zipper region (aa 381-401). Homodimerization of SREBP2 is necessary for nuclear translocation. There is one potential isoform that shows a deletion of aa 274-276 coupled to a 96 aa substitution for aa 580-1141. A second isoform (known in rodent) shows a premature truncation after Val463 and runs at 55 kDa in SDS-PAGE. Over aa 242-450, human SREBP2 shares 97% aa identity with mouse SREBP2.

Long Name

Sterol Regulatory Element Binding Transcription Factor 2

Alternate Names

bHLhd2, SREBF2

Entrez Gene IDs

6721 (Human); 20788 (Mouse); 300095 (Rat)

Gene Symbol

SREBF2

UniProt

Q12772 (Human)

Additional SREBP2 Products

Product Documents for Human SREBP2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human SREBP2 Antibody

For research use only

Citations for Human SREBP2 Antibody

Customer Reviews for Human SREBP2 Antibody (1)

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  • Human SREBP2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: iPS2 human induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 01/17/2018
    Human SREBP2 Antibody MAB7119

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Protocols

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FAQs

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Associated Pathways

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