Human ST2/IL-33 R Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human ST-2/IL-33R in direct ELISAs.
  • Source
    Monoclonal Rabbit IgG Clone # 2154B
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
     S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ST-2/ IL-33R
    Lys19-Phe328
    Accession # Q01638
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ST2/IL-33 R in HEK293 Human Cell Line Transfected with Human ST2/IL-33 R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human ST2/IL-33 R or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human ST2/IL-33 R Monoclonal Antibody (Catalog # MAB5232) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ST2/IL-33 R
ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of a 310 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 207 aa cytoplasmic domain with an intracellular TIR domain (3, 4). Alternate splicing of the 120 kDa human ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). Within the ECD, human ST2 shares 68% and 64% aa sequence identity with mouse and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5‑8). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12‑14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks the ability of IL‑33 to signal through transmembrane ST2 (10, 13‑15).
  • References:
    1. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
    2. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
    3. Tominaga, S. et al. (1992) Biochim. Biophys. Acta 1171:215.
    4. Li, H. et al. (2000) Genomics 67:284.
    5. Schmitz, J. et al. (2005) Immunity 23:479.
    6. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
    7. Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
    8. Weinberg, E.O. et al. (2002) Circulation 106:2961.
    9. Sanada S. et al. (2007) J. Clin. Invest. 117:1538.
    10. Palmer, G. et al. (2008) Cytokine 42:358.
    11. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
    12. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
    13. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
    14. Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
    15. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.
  • Long Name:
    Interleukin 33 Receptor
  • Entrez Gene IDs:
    9173 (Human); 17082 (Mouse)
  • Alternate Names:
    DER4; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33R; Ly84; ST2; ST2L; ST2V; suppression of tumorigenicity 2; T1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 40  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 18  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Rabbit IgG Antibody

WB AF008 1
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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