Human Synaptophysin Antibody

Catalog # Availability Size / Price Qty
AF5555
AF5555-SP
Human Synaptophysin Antibody in Data
2 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Human Synaptophysin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Synaptophysin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Synaptophysin
Glu50-Phe106
Accession # P08247
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat Synaptophysin antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Synaptophysin by Western Blot. Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Synaptophysin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5555) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Synaptophysin at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Synaptophysin antibody in Human Brainstem Tissue by Immunohistochemistry (IHC-P). View Larger

Synaptophysin in Human Brainstem Tissue. Synaptophysin was detected in immersion fixed paraffin-embedded sections of human brainstem tissue using Goat Anti-Human Synaptophysin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5555) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to olivary nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. *Not recommended for Immunohistochemistry/Immunocytochemistry on mouse and rat samples.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Synaptophysin

Synaptophysin (also p38 and BM89 in mouse) is a 38‑40 kDa member of the synaptophysin/synaptobrevin family of proteins. It is expressed in neurons, and interacts with VAMP2 at an early stage of vesicle formation, guiding VAMP2 placement into secretory vesicles that are destined for release upon stimulation. Human Synaptophysin is a four transmembrane protein that is 313 amino acids (aa) in length. It contains two luminal domains (aa 50‑106 and 162‑199) and a cytoplasmic C‑terminal targeting region that specifies its localization (aa 214‑313). Synaptophysin forms homodimers and oligomers, and heterodimerizes with VAMP2. There is one isoform variant that shows a six aa substitution for aa 35‑313. Over aa 50‑106, human Synaptophysin shares 89% aa identity with mouse Synaptophysin.

Entrez Gene IDs
6855 (Human)
Alternate Names
Major synaptic vesicle protein p38; MRX; MRXSYP; Synaptophysin; SYP

Product Datasheets

Citations for Human Synaptophysin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Cerebral organoids at the air-liquid interface generate diverse nerve tracts with functional output
    Authors: SL Giandomeni, SB Mierau, GM Gibbons, LMD Wenger, L Masullo, T Sit, M Sutcliffe, J Boulanger, M Tripodi, E Derivery, O Paulsen, A Lakatos, MA Lancaster
    Nat. Neurosci., 2019;22(4):669-679.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  2. Neurotransmitter Diversity In Pre-Synaptic Terminals Located in the Parvicellular Neuroendocrine Paraventricular Nucleus of the Rat and Mouse Hypothalamus
    Authors: CS Johnson, JS Bains, AG Watts
    J. Comp. Neurol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. Axonal BACE1 dynamics and targeting in hippocampal neurons: a role for Rab11 GTPase.
    Authors: Buggia-Prevot V, Fernandez C, Riordan S, Vetrivel K, Roseman J, Waters J, Bindokas V, Vassar R, Thinakaran G
    Mol Neurodegener, 2014;9(0):1.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Synaptophysin Antibody

There are currently no reviews for this product. Be the first to review Human Synaptophysin Antibody and earn rewards!

Have you used Human Synaptophysin Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review