Human TACE/ADAM17 Antibody Summary
Accession # P78536
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human TACE/ADAM17 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TACE/ADAM17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9301) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TACE/ADAM17 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TACE/ADAM17 in Human Colon. TACE/ADAM17 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human TACE/ADAM17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9301) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
TACE is a member of the ADAM family that contains A Disintegrin And Metalloprotease-like domain. Like other membrane-anchored ADAMs, TACE consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. In addition to its ability to release the 17 kDa extracellular form of tumor necrosis factor-alpha (TNF-alpha ) from the 26 kDa membrane-anchored TNF-alpha, TACE also plays an essential role in shedding ectodomains from a variety of proteins such as L-Selectin, Transforming Growth Factor-alpha, Amyloid Protein Precursor, and Notch-1 receptor. TACE mRNA is present in virtually every tissue and TACE protein resides both on the cell surface and in the cell.
- Black, R.A. and J.D. Becherer (1998) in Tumor Necrosis Factor alpha -Converting Enzyme. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1315.
- Primakoff, P. and D.G. Myles (2000) Trends in Genetics 16:83.
Citations for Human TACE/ADAM17 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Dysregulation of the ADAM17/Notch signalling pathways in endometriosis: from oxidative stress to fibrosis
Authors: I González-F, P Santulli, S Chouzenoux, F Carmona, C Chapron, F Batteux
Mol. Hum. Reprod., 2017;0(0):1-12.
Sample Types: Cell Lysates
Applications: Western Blot
ADAM-12 as a diagnostic marker for the proliferation, migration and invasion in patients with small cell lung cancer.
Authors: Shao, Shuhong, Li, Zunling, Gao, Wei, Yu, Guohua, Liu, Dexiang, Pan, Fang
PLoS ONE, 2014;9(1):e85936.
Sample Types: Whole Tissue
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