Detection of Human and Mouse TBX18 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HUVEC human umbilical vein endothelial cells, Jurkat human acute T cell leukemia cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human TBX18 Monoclonal Antibody (Catalog # MAB6337) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TBX18 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TBX18 is a 607 amino acid, 65 kDa (predicted) protein that is a member of the T-box family of developmentally expressed transcription factors. It is developmentally expressed in the anterior portion of the head, somites and limb buds. It is expressed on pluripotent progenitors in the developing epicardium where it is required for formation of the cardiac AV node, and can also contribute to fibroblast and smooth muscle formation. Within the region used as an immunogen, human TBX18 shares 93% amino acid identity with mouse and rat TBX18.
T-box Transcription Factor 18
Entrez Gene IDs:
9096 (Human); 76365 (Mouse); 315870 (Rat)
T-box 18; T-box protein 18; T-box transcription factor TBX18
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.