Human TBX6 Antibody
Human TBX6 Antibody Summary
Met1-Arg280
Accession # O95947
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human TBX6 by Western Blot. Western blot shows lysates of HT1080 human fibrosarcoma cell line and HL-60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TBX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4744) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TBX6 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
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TBX6 in Embryonic Mouse Mesoderm. TBX6 was detected in immersion fixed frozen sections of embryonic mouse mesoderm (E9.5) using 10 µg/mL Human TBX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4744) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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TBX6 in JOY6 Human iPS Cells. TBX6 was detected in immersion fixed JOY6 human induced pluripotent stem cells undifferentiated (right panel) and differentiated into mesoderm (left panel) using Goat Anti-Human TBX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4744) at 0.2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei in mesoderm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TBX6
TBX6 (T-box protein 6) is a 47 kDa member of the T-box family of transcriptional regulators. It is expressed during embryogenesis, plays a role in presomitic mesoderm development, and promotes delta-like 1 transcription. Human TBX6 is 436 amino acids (aa) in length. It contains one T‑box DNA-binding domain (aa 90‑277). There is one alternate splice form that shows a 15 aa substitution for aa 281‑436. Over aa 1‑280, human TBX6 shows 94% and 90% aa identity with canine and mouse TBX6, respectively.
Product Datasheets
Citations for Human TBX6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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A gastruloid model of the interaction between embryonic and extra-embryonic cell types
Authors: NM Bérenger-C, M Mircea, E Adegeest, PR van den Be, M Feliksik, M Hochane, T Idema, SJ Tans, S Semrau
Journal of tissue engineering, 2022;13(0):2041731422110.
Species: Mouse
Sample Types: Organoids
Applications: IHC -
Gut Microbiota Modulate CD8�T Cell Responses to Influence Colitis-Associated Tumorigenesis
Authors: AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen
Cell Rep, 2020;31(1):107471.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of eight human induced pluripotent stem cell lines from Parkinson's disease patients carrying familial mutations
Authors: M Chen, M Maimaitili, SH Buchholdt, UB Jensen, F Febbraro, M Denham
Stem Cell Res, 2019;42(0):101657.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Lineage tracing of neuromesodermal progenitors reveals novel Wnt-dependent roles in trunk progenitor cell maintenance and differentiation.
Authors: Garriock R, Chalamalasetty R, Kennedy M, Canizales L, Lewandoski M, Yamaguchi T
Development, 2015;142(9):1628-38.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity.
Authors: Gouti, Mina, Tsakiridis, Anestis, Wymeersch, Filip J, Huang, Yali, Kleinjung, Jens, Wilson, Valerie, Briscoe, James
PLoS Biol, 2014;12(8):e1001937.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
Generation of human vascular smooth muscle subtypes provides insight into embryological origin-dependent disease susceptibility.
Authors: Cheung C, Bernardo AS, Trotter MW
Nat. Biotechnol., 2012;30(2):165-73.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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