Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry, IHC-

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TBX6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TBX6
Met1-Arg280
Accession # O95947

Specificity

Detects human TBX6 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TBX6 Antibody

Detection of Human TBX6 antibody by Western Blot.

Detection of Human TBX6 by Western Blot.

Western blot shows lysates of HT1080 human fibrosarcoma cell line and HL-60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TBX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4744) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TBX6 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

TBX6 antibody in Embryonic Mouse Mesoderm by Immunohistochemistry (IHC-Fr).

TBX6 in Embryonic Mouse Mesoderm.

TBX6 was detected in immersion fixed frozen sections of embryonic mouse mesoderm (E9.5) using 10 µg/mL Human TBX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4744) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

TBX6 antibody in JOY6 Human iPS Cells by Immunocytochemistry (ICC).

TBX6 in JOY6 Human iPS Cells.

TBX6 was detected in immersion fixed JOY6 human induced pluripotent stem cells undifferentiated (right panel) and differentiated into mesoderm (left panel) using Goat Anti-Human TBX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4744) at 0.2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei in mesoderm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Detection of Mouse TBX6 by Immunocytochemistry/ Immunofluorescence

Detection of Mouse TBX6 by Immunocytochemistry/ Immunofluorescence

Generation of NMPs from EpiSCs.(A) Brachyury/Sox2 immunocytochemistry in EpiSC cultures treated with FGF/CHIR for 72 h. (B) qPCR analysis for indicated markers in mouse EpiSCs treated with FGF/CHIR. Error bars = s.d. (n = 3). n/d, not determined. Results are represented as log10 ratio of expression versus untreated EpiSCs. The data used to generate the plot can be found in Data S4. (C) Combined fluorescence/brightfield microscopy showing donor cell incorporation of grafted GFP+ EpiSC differentiated for 48 h in FGF/CHIR after 48 h embryo culture. (D) Table summarizing the incorporation of grafted GFP+ EpiSC differentiated for 24 h or 48 h in Fgf/Wnt within host embryos. NT, neural tube; Som, somite; PSM, presomitic mesoderm; n/a, not applicable. (E) Representative examples of donor cell incorporation (green, GFP) and differentiation (red, immunofluorescence for indicated markers). Cell nuclei were stained with DAPI (blue). White boxes indicate the position of magnified images of GFP+ cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25157815), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TBX6 Antibody

Application
Recommended Usage

Immunocytochemistry

0.2-15 µg/mL
Sample: Immersion fixed JOY6 human induced pluripotent stem cells differentiated into mesoderm

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse mesoderm (E9.5)

Western Blot

2 µg/mL
Sample: HT1080 human fibrosarcoma cell line, and HL‑60 human acute promyelocytic leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TBX6

TBX6 (T-box protein 6) is a 47 kDa member of the T-box family of transcriptional regulators. It is expressed during embryogenesis, plays a role in presomitic mesoderm development, and promotes delta-like 1 transcription. Human TBX6 is 436 amino acids (aa) in length. It contains one T‑box DNA-binding domain (aa 90‑277). There is one alternate splice form that shows a 15 aa substitution for aa 281‑436. Over aa 1‑280, human TBX6 shows 94% and 90% aa identity with canine and mouse TBX6, respectively.

Long Name

T-box Transcription Factor 6 [Ulnar Mammary Syndrome]

Alternate Names

DFNB67

Entrez Gene IDs

6911 (Human); 21389 (Mouse); 365371 (Rat)

Gene Symbol

TBX6

UniProt

O95947

Additional TBX6 Products

Product Documents for Human TBX6 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TBX6 Antibody

For research use only

Related Research Areas

Early Mesodermal Lineage Markers

Citations for Human TBX6 Antibody

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Protocols

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