Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 686213
View all TDRKH Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TDRKH
His206-Leu561
Accession # Q9Y2W6

Specificity

Detects human TDRKH in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human TDRD1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human TDRKH Antibody

TDRKH antibody in Adult Mouse Testis by Immunohistochemistry (IHC-Fr).

TDRKH in Adult Mouse Testis.

TDRKH was detected in immersion fixed frozen sections of adult mouse testis using Mouse Anti-Human TDRKH Monoclonal Antibody (Catalog # MAB6286) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of germ cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Human TDRKH Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed frozen sections of adult mouse testis

Reviewed Applications

Read 1 review rated 5 using MAB6286 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TDRKH

TDRKH (Tudor domain and KH domain containing protein; also TDRD2) is a 70 kDa member of the Tudor family of proteins.  It is expressed in developing spermatogonia and meiotic primary spermatocytes, but not embryonic stem cells.  TDRKH forms part of an RNA-silencing complex that safeguards the germ cell genome.  Through its 60 amino acid (aa) Tudor domain, it binds methylated PIWI (P-element-induced wimpy testis) protein and serves to position PIWI for noncoding RNA binding.  Human TDRKH is 606 aa in length.  It contains two RNA recognition KH domains (aa 52-115 and 124-190), and one methyl-binding Tudor domain (aa 353-412).  There are four potential splice forms.  Three possess a shared deletion of aa 562-606, with two of these also showing a unique deletion of aa 108-152 and 76-79, respectively; a fourth shows a two aa substitution for aa 559-606.  Over aa 206-561, human TDRKH shares 87% aa identity with mouse TDRKH.          

Long Name

Tudor and KH Domain Containing

Alternate Names

TDRD2

Entrez Gene IDs

11022 (Human); 72634 (Mouse); 310652 (Rat)

Gene Symbol

TDRKH

UniProt

Q9Y2W6 (Human)

Additional TDRKH Products

Product Documents for Human TDRKH Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TDRKH Antibody

For research use only

Related Research Areas

Primordial and Mature Germ Cells

Customer Reviews for Human TDRKH Antibody (1)

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  • Human TDRKH Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HepG2 cells
    Species: Human
    Verified Customer | Posted 02/26/2022
    Human TDRKH Antibody MAB6286

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