Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Blockade of Receptor-ligand Interaction

Cited:

Immunohistochemistry-Frozen, Western Blot, Neutralization, Surface Plasmon Resonance

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TfR (Transferrin R) Primary Antibodies »

Product Specifications

Immunogen

Human placenta-derived TfR (Transferrin R)

Specificity

Detects human TfR (Transferrin R) in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human TfR (Transferrin R) Antibody

Detection of Human TfR (Transferrin R) antibody by Western Blot.

Detection of Human TfR (Transferrin R) by Western Blot.

Western blot shows lysates of ZR-75 human breast cancer cell line, U937 human histiocytic lymphoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human TfR (Transferrin R) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2474) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TfR (Transferrin R) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TfR (Transferrin R) antibody in Human Liver by Immunohistochemistry (IHC-P).

TfR (Transferrin R) in Human Liver.

TfR (Transferrin R) was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human TfR (Transferrin R) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2474) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Mouse TfR (Transferrin R) by Western Blot

Detection of Mouse TfR (Transferrin R) by Western Blot

Effect of Pcsk9 deletion on LDLR and CD81 levels in age- and sex-matched Pcsk9-/- mice (n = 5) and their wild type littermates (n = 4).Shown are (A) mean ± SE serum LDL-C levels and (B) Western blot analysis of CD81 and LDLR levels in liver extracts with TfR as loading control, in animals sacrificed after a 4-hour fast. Lanes in panel B represent samples from individual mice. LDL-C, low-density lipoprotein cholesterol; LDLR, low-density lipoprotein receptor; PCSK9, proprotein convertase subtilisin/kexin type 9; SE, standard error; TfR, transferrin receptor; wt, wild type. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0154498), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of TfR (Transferrin R) by Western Blot

Detection of TfR (Transferrin R) by Western Blot

shFXN have altered iron metabolism proteins. RNA was reverse transcribed and amplified for (A) Transferrin receptor (TfR), (B) Ferroportin (Fpn), and Beta-2-microglobulin (B2M) as a housekeeping control, transcript abundance was quantified using the delta delta Ct method normalized to the empty vector endothelial cells (EVEC). Total protein lysates were run on a 12% bis-tris SDS-PAGE, transferred to nitrocellulose, and probed for (C) TfR and (D) Fpn protein expression against Tata-Binding Protein (TBP) as a housekeeping gene. Each protein is normalized to that of TBP as quantified by densitometry, normalized to pooled EVEC values. (E) Representative blot is shown. TfR is visualized with Alexa-647 (Red), and Fpn with Alexa-488 (Green). Student’s t-test alpha = 0.05. *p < 0.05, **p < 0.01. (A) EVEC; n = 2 shFXN; n = 3. (B) EVEC; n = 1, and shFXN2; n = 2. (C–E) EVEC; n = 6 and shFXN; n = 8. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fmolb.2023.1299201/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TfR (Transferrin R) Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.1-0.5 µg/mL of this antibody will block 50% of the binding of 25 ng/mL of Recombinant Human TfR (Transferrin R) (Catalog # 2474-TR) to immobilized human Holo-Transferrin (Catalog # 2914-HT) coated at 500 ng/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human bladder, breast, and liver

Western Blot

0.25 µg/mL
Sample: ZR‑75 human breast cancer cell line, U937 human histiocytic lymphoma cell line, and K562 human chronic myelogenous leukemia cell line

Reviewed Applications

Read 4 reviews rated 4.5 using AF2474 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: TfR (Transferrin R)

The Transferrin Receptor (TfR or TfR-1, designated CD71) is a type 2 transmembrane glycoprotein expressed on erythroid progenitors, muscle cells and proliferating cells as a 188 kDa disulfide-linked homodimer of 95 kDa monomers (1-4). As the major mediator of cellular iron uptake, it binds and internalizes diferric transferrin, allowing iron release at the low pH of the endosome (2, 5). The human TfR cDNA encodes 760 amino acids (aa) including a 67 aa N-terminal intracellular domain, a 21 aa transmembrane domain, and a 672 aa extracellular domain (ECD) with helical, peptidase (nonfunctional), and ligand binding domains, including an RGD potential integrin binding site (5). Human TfR ECD shares 75 - 80% aa identity with mouse, rat, feline, canine, equine, porcine and bovine TfR. A 679 aa alternately spliced form begins at aa 82 and is presumably secreted, while in an 804 aa form, 44 aa are inserted at aa 518 within the peptidase region (6). Most soluble TfR (sTfR) arises from trypsin proteolysis at aa 100, producing the circulating form of TfR (3). sTfR concentration in plasma or serum is proportional to total TfR and can be increased by iron deficiency (3). Erythroid progenitors, which use iron for hemoglobin synthesis, normally account for the bulk of total body TfR production (3). Since rapidly growing cells require iron to replicate DNA, cancer cells can express up to 5-fold more TfR than quiescent cells in the surrounding tissue (2, 4). Antibody targeting of TfR can inhibit tumor cell proliferation and induce apoptosis (2, 4). The hereditary hemochromatosis protein HFE competes with diferric transferrin for binding to TfR, and targets TfR for degradation rather than recycling (2, 5). TfR has been reported to have ferritin-independent functions in T cell development, immunological synapse formation and galectin-3-mediated cell death, and to be a cell entry receptor for New World hemorrhagic fever arenaviruses (2, 4, 7).

References

  1. Schneider, C. et al. (1984) Nature 311:675.
  2. Daniels, T.R. et al. (2006) Clin. Immunol. 121:144.
  3. Skikne, B.S. (2008) Am. J. Hematol. 83:872.
  4. Macedo, M.F. and M. deSousa (2008) Inflamm. Allergy Drug Targets 7:41.
  5. Aisen, P. (2004) Int. J. Biochem. Cell Biol. 36:2137.
  6. Entrez protein Accession # EAW53671, EAW53672.
  7. Radoshitzky, S.R. et al. (2007) Nature 446:92.

Long Name

Transferrin Receptor

Alternate Names

CD71, TfR (TransferrinR), TFR1, TFRC, TRFR

Entrez Gene IDs

7037 (Human); 22042 (Mouse); 64678 (Rat)

Gene Symbol

TFRC

Additional TfR (Transferrin R) Products

Product Documents for Human TfR (Transferrin R) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human TfR (Transferrin R) Antibody

For research use only

Citations for Human TfR (Transferrin R) Antibody

Customer Reviews for Human TfR (Transferrin R) Antibody (4)

4.5 out of 5
4 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human TfR (Transferrin R) Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 4 of 4 reviews Showing All
Filter By:
Clear Filters X
  • Human TfR (Transferrin R) Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: K562 human chronic myelogenous leukemia cell line
    Species: cell
    Verified Customer | Posted 12/11/2025
    1:100 dilution
    Human TfR (Transferrin R) Antibody AF2474
  • Human TfR (Transferrin R) Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Heparin Plasma
    Species: Human
    Verified Customer | Posted 05/12/2021
    Coated wells with this antibody to measure transferrin receptor in an immunoassay.
    Human TfR (Transferrin R) Antibody AF2474
  • Human TfR (Transferrin R) Antibody
    Name: Teddy Chan
    Application: Western Blot
    Sample Tested: Brain Vascular Smooth Muscle Cells
    Species: Human
    Verified Customer | Posted 02/08/2019
    Human TfR (Transferrin R) Antibody AF2474
  • Human TfR (Transferrin R) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa cells, PC-3 human prostate cancer cell line, MCF-7 human breast cancer cell line and MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 07/23/2018
    Total cell lysates from HeLa, PC-3, MCF-7 and MDA-MB-231 were subjected to western blot. PVDF membrane were probed with 0.5 um/ml Human TfR Antibody (AF2474). A specific band was detected for TfR at approximately 90 kDa. This experiment was conducted under reducing conditions
    Human TfR (Transferrin R) Antibody AF2474

There are no reviews that match your criteria.

Showing  1 - 4 of 4 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies