TGIF1 in Human Squamous Cell Carcinoma.
TGIF1 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human TGIF1 Monoclonal Antibody (Catalog # MAB75551) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TGIF1 (5'-TG[TCA]-3' Interacting Protein 1) also known as Transforming Growth Factor beta-induced Factor 1 and HPE4, is a 43 kDa (predicted) member of the TALE/TGIF homeobox family of proteins. It is widely expressed, and serves as a transcriptional corepressor for SMAD2-induced gene activation. Evidence suggests that it either complexes with c-jun and cPML, or interacts with HDAC in the nucleus to perform this function. It also binds to RXR response elements, blocking RXR-mediated gene activation. Such interactions are of considerable importance during fetal and neonatal development. Human TGIF1 is 401 amino acids (aa) in length. It contains a DNA-binding homeobox domain (aa 164-226) and two consecutive repressor domains (aa 267-401). There are multiple splice variants. One is very well characterized, is 33-35 kDa in size, and shows a four aa substitution for aa 1-133. This is most active when phosphorylated, which adds approximately 2-4 kDa to its SDS-PAGE MW. Two other splice forms contain a 19 aa substitution for aa 1-134, and utilize an alternative start site at Met150, respectively. Over aa 245-359, human TGIF1 shares 81% aa sequence identity with mouse TGIF1.
TGFB-induced Factor Homeobox 1
Entrez Gene IDs:
7050 (Human); 21815 (Mouse); 316742 (Rat)
5'-TG-3' interacting factor; homeobox protein TGIF; homeobox protein TGIF1; HPE4; MGC39747; TALE homeobox TG-interacting factor; TGFB-induced factor (TALE family homeobox); TGFB-induced factor homeobox 1; TGIF1; TGIFMGC5066,5'-TG-3'-interacting factor 1; transforming growth factor-beta-induced factor
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