Human TGIF1 Antibody

Catalog # Availability Size / Price Qty
TGIF1 in Human Squamous Cell Carcinoma.
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Product Details
Supplemental Products

Human TGIF1 Antibody Summary

Species Reactivity
Detects human TGIF1 in direct ELISAs.
Monoclonal Mouse IgG2A Clone # 790715
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human TGIF1
Accession # Q15583
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry TGIF1 antibody in Human Squamous Cell Carcinoma by Immunohistochemistry (IHC-P). View Larger

TGIF1 in Human Squamous Cell Carcinoma. TGIF1 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human TGIF1 Monoclonal Antibody (Catalog # MAB75551) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGIF1

TGIF1 (5'-TG[TCA]-3' Interacting Protein 1) also known as Transforming Growth Factor beta-induced Factor 1 and HPE4, is a 43 kDa (predicted) member of the TALE/TGIF homeobox family of proteins. It is widely expressed, and serves as a transcriptional corepressor for SMAD2-induced gene activation. Evidence suggests that it either complexes with c-jun and cPML, or interacts with HDAC in the nucleus to perform this function. It also binds to RXR response elements, blocking RXR-mediated gene activation. Such interactions are of considerable importance during fetal and neonatal development. Human TGIF1 is 401 amino acids (aa) in length. It contains a DNA-binding homeobox domain (aa 164-226) and two consecutive repressor domains (aa 267-401). There are multiple splice variants. One is very well characterized, is 33-35 kDa in size, and shows a four aa substitution for aa 1-133. This is most active when phosphorylated, which adds approximately 2-4 kDa to its SDS-PAGE MW. Two other splice forms contain a 19 aa substitution for aa 1-134, and utilize an alternative start site at Met150, respectively. Over aa 245-359, human TGIF1 shares 81% aa sequence identity with mouse TGIF1.

Long Name
TGFB-induced Factor Homeobox 1
Entrez Gene IDs
7050 (Human); 21815 (Mouse); 316742 (Rat)
Alternate Names
5'-TG-3' interacting factor; homeobox protein TGIF; homeobox protein TGIF1; HPE4; MGC39747; TALE homeobox TG-interacting factor; TGFB-induced factor (TALE family homeobox); TGFB-induced factor homeobox 1; TGIF1; TGIFMGC5066,5'-TG-3'-interacting factor 1; transforming growth factor-beta-induced factor

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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