Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2116D
View all TL1A/TNFSF15 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TL1A/TNFSF15
Leu72-Leu251
Accession # O95150

Specificity

Detects human TL1A/TNFSF15 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human TL1A/TNFSF15 Antibody

Detection of Human TL1A/TNFSF15 antibody by Western Blot.

Detection of Human TL1A/TNFSF15 by Western Blot.

Western blot shows lysates of human prostate tissue, human lung tissue, and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human TL1A/ TNFSF15 Monoclonal Antibody (Catalog # MAB74421) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for TL1A/TNFSF15 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TL1A/TNFSF15 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P).

TL1A/TNFSF15 in Human Prostate Cancer Tissue.

TL1A/TNFSF15 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Rabbit Anti-Human TL1A/TNFSF15 Monoclonal Antibody (Catalog # MAB74421) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Apoptosis Induced by TL1A/TNFSF15 and Neutralization by Human TL1A/TNFSF15 Antibody.

Apoptosis Induced by TL1A/TNFSF15 and Neutralization by Human TL1A/TNFSF15 Antibody.

Recombinant Human TL1A/TNFSF15 (Catalog # 1319-TL) induces apoptosis in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Apoptosis elicited by Recombinant Human TL1A/TNFSF15 (80 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human TL1A/ TNFSF15 Monoclonal Antibody (Catalog # MAB74421). The ND50 is typically 0.04-0.2 ug/mL.

Applications for Human TL1A/TNFSF15 Antibody

Application
Recommended Usage

Immunohistochemistry

0.3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Western Blot

1 µg/mL
Sample: Human prostate tissue, human lung tissue, and HUVEC human umbilical vein endothelial cells

Neutralization

Measured by its ability to neutralize TL1A/TNFSF15-induced apoptosis in the TF‑1 human erythroleukemic cell line. The Neutralization Dose (ND50) is typically 0.04-0.2 ug/mL in the presence of 80 ng/mL Recombinant Human TL1A/TNFSF15.

Reviewed Applications

Read 1 review rated 5 using MAB74421 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TL1A/TNFSF15

TL1A is a type II transmembrane protein belonging to the TNF superfamily and has been designated TNF superfamily member 15 (TNFSF15). Human TL1A is a 251 aa protein consisting of a 35 aa cytoplasmic domain, a 24 aa transmembrane region and a 192 aa C-terminal extracellular domain. It is a longer variant of the previously cloned TL1 (also known as VEGI) that is possibly a cloning artifact. TL1A is predominantly expressed in endothelial cells and its expression is inducible by TNF-alpha and IL-1 alpha. TL1A binds with high affinity to death receptor 3 (DR3), which is now designated TNF receptor superfamily member 25 (TNFRSF25). DR3 was formerly designated TNFRSF12 when it was thought to be the receptor for TWEAK/TNFSF12. DR3 is expressed primarily on activated T cells. Depending on the cell context, ligation of DR3 by TL1A can trigger one of two signaling pathways, activation of the transcription factor NF-kappa-B or activation of caspases and apoptosis. On primary T cells, TL1A induces NF-kappa-B activation and a costimulatory signal to increase IL-2 responsiveness and the secretion of proinflammatory cytokines. However, in a tumor cell line, TF-1, TL1A has been shown to induce caspase activity and apoptosis. These effects of TL1A are blocked by the secreted, soluble decoy receptor 3 (DcR3), also known as TR6 and TNFRSF6B, which compete with DR3 for binding to TL1A. Consistent with the observed in vitro activities, TL1A promotes ex vivo splenocyte expansion and enhances in vivo graft-versus-host-response.

References

  1. Migone, T.S. et al. (2002) Immunity 16:479.

Long Name

TNF-like 1

Alternate Names

TNFSF15, VEGI

Entrez Gene IDs

9966 (Human); 326623 (Mouse)

Gene Symbol

TNFSF15

UniProt

O95150

Additional TL1A/TNFSF15 Products

Product Documents for Human TL1A/TNFSF15 Antibody

Certificate of Analysis

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Product Specific Notices for Human TL1A/TNFSF15 Antibody

For research use only

Customer Reviews for Human TL1A/TNFSF15 Antibody (1)

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  • TL1A/TNFSF15 Antibody Review
    Name: Md Imtiazul Islam
    Application: Western Blot
    Sample Tested: Monocytes
    Verified Customer | Posted 01/13/2026
    Western blot for TNFSF-15 Dectection
    I used this antibody to detect TNFSF 15 in endothelial cell with western blot in 1:1000 dilution and it worked very well.
    Human TL1A/TNFSF15 Antibody MAB74421

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