Human TR2/NR2C1 Antibody

Catalog #: PP-H0037-00 Datasheet / COA / SDS
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PP-H0037-00
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Human TR2/NR2C1 Antibody Summary

Species Reactivity
Human
Specificity
This antibody specifically recognizes human TR2. Not yet tested in other species.
Source
Monoclonal Mouse IgG2B Clone # H0037
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant human TR2/NR2C1
aa 4-110
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
This antibody can be used at 2 μg/mL under reducingconditions with the appropriate secondary reagents to detect human TR2.
 
Immunoprecipitation
Optimal dilutions should be determined by eachlaboratory.
 
Direct ELISA
This antibody can be used at 0.2 μg/mL with the appropriatesecondary reagents to detect human TR2.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: TR2/NR2C1

Human Testicular Receptor 2 (TR2, TR2-11; NR2C1) is a 67 - 70 kDa, 603 amino acid member of the NR2 subfamily of the Nuclear Hormone Receptor superfamily. It is expressed in prostate and testis, forms homodimers with itself, and heterodimers with TR4. In addition to TR2-11, there are three other isoforms. All exhibit a DNA binding domain (DBD) (amino acids 111 - 179) and a variable length ligand binding domain (LBD) (amino acids 261 – 603). TR2-7 is truncated at 184 amino acids in length, with amino acids 1 - 181 showing complete identity to TR2-11. There is no LBD. TR2-9 is 467 amino acids in length and is identical to TR2-11 over amino acids 1 – 464. TR2-5 is 483 amino acids in length and identical to TR2-11 over amino acids 1 - 466. Their significance is unclear.

Long Name
Testicular Orphan Nuclear Receptor 2
Entrez Gene IDs
7181 (Human)
Alternate Names
NR2C1; nuclear receptor subfamily 2 group C member 1; nuclear receptor subfamily 2, group C isoform; nuclear receptor subfamily 2, group C, member 1; Orphan nuclear receptor TR2; Testicular receptor 2; TR2 nuclear hormone receptor; TR2; TR2-11

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