Detection of Human TRADD by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, MCF‑7 human breast cancer cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRADD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2658) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRADD at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TNF RI (Tumor Necrosis Factor Receptor I) activation leads to the recruitment of the adapter protein TRADD (TNF RI-associated Death Domain) to the cytoplasmic death domain of TNF RI. The TRADD protein also contains a death domain with sequence similarity to that of TNF RI. In addition to the initiation of signaling events leading to apoptosis, the recruitment of TRADD or over-expression of the TRADD protein also activates the transcription factor NFkB.
TNFRSF1A-associated via Death Domain
Entrez Gene IDs:
MGC11078; TNFRSF1A-associated via death domainTNFR1-associated DEATH domain protein; TRADD; tumor necrosis factor receptor type 1 associated death domain protein; tumor necrosis factor receptor type 1-associated DEATH domain protein; tumor necrosis factor receptor-1-associated protein
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