Human TWEAK R/TNFRSF12 Antibody

Catalog # Availability Size / Price Qty
AF1199
AF1199-SP
TWEAK R/TNFRSF12 Inhibition of TWEAK/TNFSF12-depend­ent Cell Proliferation and Neutralization by Human TWEAK R/TNFRSF12 Anti­body.
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Human TWEAK R/TNFRSF12 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TWEAK R/TNFRSF12 in direct ELISAs and Western blots. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse TWEAK R/TNFRSF12 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
recombinant human TWEAK R/TNFRSF12
Glu28-Trp79
Accession # Q9NP84
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TWEAK R/TNFRSF12 Fc Chimera (Catalog # 1199-TW)
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human liver cancer tissue
Neutralization
Measured by its ability to neutralize TWEAK R/TNFRSF12-mediated inhibition of proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 0.3-1.2 µg/mL in the presence of 30 ng/mL Recombinant Human TWEAK R/TNFRSF12 Fc Chimera and 20 ng/mL Recombinant Human TWEAK/TNFSF12.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization TWEAK R/TNFRSF12 Inhibition of TWEAK/TNFSF12-depend­ent Cell Proliferation and Neutralization by Human TWEAK R/TNFRSF12 Anti­body. View Larger

TWEAK R/TNFRSF12 Inhibition of TWEAK/TNFSF12-depend­ent Cell Proliferation and Neutralization by Human TWEAK R/TNFRSF12 Anti­body.

Recombinant Human TWEAK R/TNFRSF12 Fc Chimera (Catalog # 1199‑TW) inhibits Recombinant Human TWEAK/TNFSF12 (Catalog # 1090‑TW) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human TWEAK/TNFSF12 (20 ng/mL) activity elicited by Recombinant Human TWEAK R/TNFRSF12 Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TWEAK R/TNFRSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1199). The ND50 is typically 0.3-1.2 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TWEAK R/TNFRSF12

The gene for TNF-related weak inducer of apoptosis receptor (TWEAK R) was originally identified as a fibroblast growth factor-inducible immediate-early response gene Fn14 in mouse NIH 3T3 fibroblasts (1, 2). Human TWEAK R cDNA encodes a 129 amino acid (aa) residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain (1‑3). Human and mouse TWEAK R share 82% aa sequence identity. TWEAK R is the smallest member of the TNF receptor superfamily and contains only one cysteine-rich region in its extracellular domain. The TWEAK R cytoplasmic domain conatins one TRAF binding motif which binds TRAFs 1, 2, and 3. TWEAK R binds its ligand TWEAK/TNFSF12 with high affinity to initiate a signal transduction cascade that depending upon the cell type, may lead to a variety of cellular responses including cell death, cell proliferation, and angiogenesis (2‑6). In new born mice, TWEAK R is highly expressed in all tissues examined (heart, intestine, kidney, liver, lung and skin) (1). In adult mice, high TWEAK R expression levels are found in the heart and ovary, while lower expression levels are detected in the lung, kidney, skin. Elevated levels of TWEAK R mRNA were found in human or mouse hepatocellular carcinoma specimens, in regenerating mouse liver and in injured rat arteries (2, 3).

References
  1. Meighan-Mantha, R. et al. (1999) J. Biol. Chem. 274:33166.
  2. Feng, S. et al. (2000) Am J. Pathol. 156:1253.
  3. Wiley, S. et al. (2001) Immunity 15:837.
  4. Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.
  5. Nakayama, M. et al. (2002) J. Immunol. 168:734.
  6. Lynch, C.N. et al. (1999) J. Biol. Chem. 274:8455.
Long Name
TNF-like and Weak Inducer of Apoptosis Receptor
Entrez Gene IDs
51330 (Human); 27279 (Mouse)
Alternate Names
CD266 antigen; CD266; FGF-inducible 14; Fibroblast growth factor-inducible immediate-early response protein 14; Fn14; FN14tumor necrosis factor receptor superfamily member 12A; TNFRSF12; TNFRSF12A; tumor necrosis factor receptor superfamily, member 12A; TWEAK R; TWEAKR; tweak-receptor; type I transmembrane protein Fn14

Product Datasheets

Citations for Human TWEAK R/TNFRSF12 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. TWEAK/Fn14 system and crescent formation in IgA nephropathy.
    Authors: Sasaki Y, Shimizu Y, Suzuki Y, Horikoshi S, Tomino Y
    BMC Nephrol, 2015;16(0):27.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC - paraffin
  2. Lipocalin 2, the TNF-like receptor TWEAKR and its ligand TWEAK act downstream of NFAT1 to regulate breast cancer cell invasion.
    Authors: Gaudineau B, Fougere M, Guaddachi F, Lemoine F, de la Grange P, Jauliac S
    J Cell Sci, 2012;125(0):4475-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human TWEAK R/TNFRSF12 Antibody

There are currently no reviews for this product. Be the first to review Human TWEAK R/TNFRSF12 Antibody and earn rewards!

Have you used Human TWEAK R/TNFRSF12 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review