Human TWEAK R/TNFRSF12 Antibody Summary
Accession # Q9NP84
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
TWEAK R/TNFRSF12 Inhibition of TWEAK/TNFSF12-dependent Cell Proliferation and Neutralization by Human TWEAK R/TNFRSF12 Antibody.
Recombinant Human TWEAK R/TNFRSF12 Fc Chimera (Catalog # 1199‑TW) inhibits Recombinant Human TWEAK/TNFSF12 (Catalog # 1090‑TW) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human TWEAK/TNFSF12 (20 ng/mL) activity elicited by Recombinant Human TWEAK R/TNFRSF12 Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TWEAK R/TNFRSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1199). The ND50 is typically 0.3-1.2 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TWEAK R/TNFRSF12
The gene for TNF-related weak inducer of apoptosis receptor (TWEAK R) was originally identified as a fibroblast growth factor-inducible immediate-early response gene Fn14 in mouse NIH 3T3 fibroblasts (1, 2). Human TWEAK R cDNA encodes a 129 amino acid (aa) residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain (1‑3). Human and mouse TWEAK R share 82% aa sequence identity. TWEAK R is the smallest member of the TNF receptor superfamily and contains only one cysteine-rich region in its extracellular domain. The TWEAK R cytoplasmic domain conatins one TRAF binding motif which binds TRAFs 1, 2, and 3. TWEAK R binds its ligand TWEAK/TNFSF12 with high affinity to initiate a signal transduction cascade that depending upon the cell type, may lead to a variety of cellular responses including cell death, cell proliferation, and angiogenesis (2‑6). In new born mice, TWEAK R is highly expressed in all tissues examined (heart, intestine, kidney, liver, lung and skin) (1). In adult mice, high TWEAK R expression levels are found in the heart and ovary, while lower expression levels are detected in the lung, kidney, skin. Elevated levels of TWEAK R mRNA were found in human or mouse hepatocellular carcinoma specimens, in regenerating mouse liver and in injured rat arteries (2, 3).
- Meighan-Mantha, R. et al. (1999) J. Biol. Chem. 274:33166.
- Feng, S. et al. (2000) Am J. Pathol. 156:1253.
- Wiley, S. et al. (2001) Immunity 15:837.
- Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.
- Nakayama, M. et al. (2002) J. Immunol. 168:734.
- Lynch, C.N. et al. (1999) J. Biol. Chem. 274:8455.
Citations for Human TWEAK R/TNFRSF12 Antibody
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Citations: Showing 1 - 2
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TWEAK/Fn14 system and crescent formation in IgA nephropathy.
Authors: Sasaki Y, Shimizu Y, Suzuki Y, Horikoshi S, Tomino Y
BMC Nephrol, 2015;16(0):27.
Sample Types: Whole Tissue
Applications: IHC - paraffin
Lipocalin 2, the TNF-like receptor TWEAKR and its ligand TWEAK act downstream of NFAT1 to regulate breast cancer cell invasion.
Authors: Gaudineau B, Fougere M, Guaddachi F, Lemoine F, de la Grange P, Jauliac S
J Cell Sci, 2012;125(0):4475-86.
Sample Types: Whole Cells
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