Human TWEAK R/TNFRSF12 Antibody AF1199: R&D Systems

Human TWEAK R/TNFRSF12 Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human TWEAK R/TNFRSF12 in direct ELISAs and Western blots. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse TWEAK R/TNFRSF12 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    recombinant human TWEAK R/TNFRSF12
    Glu28-Trp79
    Accession # Q9NP84
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human TWEAK R/TNFRSF12 Fc Chimera (Catalog # 1199-TW)
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed paraffin-embedded sections of human liver cancer tissue
  • Neutralization
    Measured by its ability to neutralize TWEAK R/TNFRSF12-mediated inhibition of proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 0.3-1.2 µg/mL in the presence of 30 ng/mL Recombinant Human TWEAK R/TNFRSF12 Fc Chimera and 20 ng/mL Recombinant Human TWEAK/TNFSF12.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
TWEAK R/TNFRSF12 Inhibition of TWEAK/TNFSF12-depend­ent Cell Proliferation and Neutralization by Human TWEAK R/TNFRSF12 Anti­body.

Recombinant Human TWEAK R/TNFRSF12 Fc Chimera (Catalog # 1199‑TW) inhibits Recombinant Human TWEAK/TNFSF12 (Catalog # 1090‑TW) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human TWEAK/TNFSF12 (20 ng/mL) activity elicited by Recombinant Human TWEAK R/TNFRSF12 Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TWEAK R/TNFRSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1199). The ND50 is typically 0.3-1.2 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TWEAK R/TNFRSF12

The gene for TNF-related weak inducer of apoptosis receptor (TWEAK R) was originally identified as a fibroblast growth factor-inducible immediate-early response gene Fn14 in mouse NIH 3T3 fibroblasts (1, 2). Human TWEAK R cDNA encodes a 129 amino acid (aa) residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain (1‑3). Human and mouse TWEAK R share 82% aa sequence identity. TWEAK R is the smallest member of the TNF receptor superfamily and contains only one cysteine-rich region in its extracellular domain. The TWEAK R cytoplasmic domain conatins one TRAF binding motif which binds TRAFs 1, 2, and 3. TWEAK R binds its ligand TWEAK/TNFSF12 with high affinity to initiate a signal transduction cascade that depending upon the cell type, may lead to a variety of cellular responses including cell death, cell proliferation, and angiogenesis (2‑6). In new born mice, TWEAK R is highly expressed in all tissues examined (heart, intestine, kidney, liver, lung and skin) (1). In adult mice, high TWEAK R expression levels are found in the heart and ovary, while lower expression levels are detected in the lung, kidney, skin. Elevated levels of TWEAK R mRNA were found in human or mouse hepatocellular carcinoma specimens, in regenerating mouse liver and in injured rat arteries (2, 3).

  • References:
    1. Meighan-Mantha, R. et al. (1999) J. Biol. Chem. 274:33166.
    2. Feng, S. et al. (2000) Am J. Pathol. 156:1253.
    3. Wiley, S. et al. (2001) Immunity 15:837.
    4. Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.
    5. Nakayama, M. et al. (2002) J. Immunol. 168:734.
    6. Lynch, C.N. et al. (1999) J. Biol. Chem. 274:8455.
  • Long Name:
    TNF-like and Weak Inducer of Apoptosis Receptor
  • Entrez Gene IDs:
    51330 (Human); 27279 (Mouse)
  • Alternate Names:
    CD266 antigen; CD266; FGF-inducible 14; Fibroblast growth factor-inducible immediate-early response protein 14; Fn14; FN14tumor necrosis factor receptor superfamily member 12A; TNFRSF12; TNFRSF12A; tumor necrosis factor receptor superfamily, member 12A; TWEAK R; TWEAKR; tweak-receptor; type I transmembrane protein Fn14
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. TWEAK/Fn14 system and crescent formation in IgA nephropathy.
    Authors: Sasaki Y, Shimizu Y, Suzuki Y, Horikoshi S, Tomino Y
    BMC Nephrol, 2015;16(0):27.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - paraffin
  2. Lipocalin 2, the TNF-like receptor TWEAKR and its ligand TWEAK act downstream of NFAT1 to regulate breast cancer cell invasion.
    Authors: Gaudineau B, Fougere M, Guaddachi F, Lemoine F, de la Grange P, Jauliac S
    J Cell Sci, 2012;125(0):4475-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
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