Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Bovine, Fish - Danio rerio (Zebrafish), Zebrafish

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry, Simple Western, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, ELISA Capture, IF/ICC, RNAscope compatible, Westen Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all VE-Cadherin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human VE-Cadherin
Asp48-Gln593
Accession # P33151

Specificity

Detects human VE-Cadherin in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse VE‑Cadherin is observed and less than 5% cross-reactivity with recombinant human E-Cadherin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human VE‑Cadherin Antibody

Detection of Human VE-Cadherin antibody by Western Blot.

Detection of Human VE‑Cadherin by Western Blot.

Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human VE-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for VE-Cadherin at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
VE-Cadherin antibody in HUVEC Cells by Immunocytochemistry (ICC).

VE‑Cadherin in HUVEC Cells.

VE-Cadherin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human VE-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human VE‑Cadherin by Simple WesternTM.

Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for VE‑Cadherin at approximately 162 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Human VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of VE-Cadherin by Western Blot

Detection of VE-Cadherin by Western Blot

Treatment with an NO donor or Claudin-1 inhibition improved barrier integrity in ASL-deficient HBMECs.(A) TEER measurement in HBMECs treated with 0, 10, or 25 μM of S-nitroso-N-acetylpenicillamine (SNAP), an NO donor, 48 hours (hr) after subculture (n = 3). (B) Comparison of TEER levels in HBMECs treated with 25 μM of either N-acetylpenicillamine (NAP) or SNAP 48 hr after subculture. SNAP or NAP was added daily, starting 24 hr after subculture (n = 3). (C) Western blot analysis and (D) quantification investigating the effects of SNAP (25 μM) on expression of ASL, CLDN5, CLDN1, and VE-Cadherin in ASL-deficient HBMECs (n = 3). SNAP was added to the treatment group 24 hr prior to protein extraction. (E) Relative mRNA expression of CLDN1 in HBMECs 48 hr after transfection with siControl, siASL, or both siASL and siCLDN1 (n = 3). (F) Western blot analysis and (G) quantification to assess ASL, CLDN5, and CLDN1 protein level in HBMECs 48 hr after siRNA transfection (n = 3). Protein abundance was normalized to alpha -Tubulin level in D and G. (H) TEER measurement to investigate the effects of SNAP (25 μM) or siRNA-mediated inhibition of CLDN1 on paracellular barrier integrity of ASL-deficient HBMECs. Bar graphs represent mean values while error bars represent the standard deviation. *P < 0.05, **P < 0.01, and ***P < 0.001. One-way ANOVA, multiple comparisons. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37490345), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of VE-Cadherin by Western Blot

Detection of VE-Cadherin by Western Blot

Treatment with an NO donor or Claudin-1 inhibition improved barrier integrity in ASL-deficient HBMECs.(A) TEER measurement in HBMECs treated with 0, 10, or 25 μM of S-nitroso-N-acetylpenicillamine (SNAP), an NO donor, 48 hours (hr) after subculture (n = 3). (B) Comparison of TEER levels in HBMECs treated with 25 μM of either N-acetylpenicillamine (NAP) or SNAP 48 hr after subculture. SNAP or NAP was added daily, starting 24 hr after subculture (n = 3). (C) Western blot analysis and (D) quantification investigating the effects of SNAP (25 μM) on expression of ASL, CLDN5, CLDN1, and VE-Cadherin in ASL-deficient HBMECs (n = 3). SNAP was added to the treatment group 24 hr prior to protein extraction. (E) Relative mRNA expression of CLDN1 in HBMECs 48 hr after transfection with siControl, siASL, or both siASL and siCLDN1 (n = 3). (F) Western blot analysis and (G) quantification to assess ASL, CLDN5, and CLDN1 protein level in HBMECs 48 hr after siRNA transfection (n = 3). Protein abundance was normalized to alpha -Tubulin level in D and G. (H) TEER measurement to investigate the effects of SNAP (25 μM) or siRNA-mediated inhibition of CLDN1 on paracellular barrier integrity of ASL-deficient HBMECs. Bar graphs represent mean values while error bars represent the standard deviation. *P < 0.05, **P < 0.01, and ***P < 0.001. One-way ANOVA, multiple comparisons. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37490345), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of VE-Cadherin by Western Blot

Detection of VE-Cadherin by Western Blot

ASL knockdown in HBMECs leads to reduced NO signaling, decreased paracellular permeability, and dysregulated expression of BBB-associated genes.(A) Relative mRNA expression of ASL in HBMECs 2 days after transfection with control and ASL siRNAs (siControl and siASL) (n = 3). (B) Intracellular levels of NO in siControl and siASL-transfected HBMECs as measured by DAF-FM diacetate fluorescence assay (n = 9). (C) Intracellular cGMP levels in siControl and siASL-transfected HBMECs as measured by ELISA (n = 5). (D) Quantitative analysis of TEER level at 1–3 days after transfected HBMECs were seeded on Transwell inserts (n = 3). (E) Relative mRNA expression of CLDN5, CDH5, TJP1, OCLN, and CLDN1 in HBMECs 2 days after siRNA-mediated knockdown (n = 3). (F) Western blot analysis of ASL, CLDN1, OCLN, CLDN5, and VE-Cadherin protein levels in HBMECs 2 days after siControl and siASL transfection. Samples were run on 2 separate gels, and alpha -Tubulin was used as the internal control (n = 3). (G) Protein abundance was quantitatively measured after normalization to alpha -Tubulin level. Bar graphs represent mean values while error bars represent the standard deviation. *P < 0.05, **P < 0.01, and ***P < 0.001. Student’s t test. A.U., arbitrary units. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37490345), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of VE-Cadherin by Western Blot

Detection of VE-Cadherin by Western Blot

ASL knockdown in HBMECs leads to reduced NO signaling, decreased paracellular permeability, and dysregulated expression of BBB-associated genes.(A) Relative mRNA expression of ASL in HBMECs 2 days after transfection with control and ASL siRNAs (siControl and siASL) (n = 3). (B) Intracellular levels of NO in siControl and siASL-transfected HBMECs as measured by DAF-FM diacetate fluorescence assay (n = 9). (C) Intracellular cGMP levels in siControl and siASL-transfected HBMECs as measured by ELISA (n = 5). (D) Quantitative analysis of TEER level at 1–3 days after transfected HBMECs were seeded on Transwell inserts (n = 3). (E) Relative mRNA expression of CLDN5, CDH5, TJP1, OCLN, and CLDN1 in HBMECs 2 days after siRNA-mediated knockdown (n = 3). (F) Western blot analysis of ASL, CLDN1, OCLN, CLDN5, and VE-Cadherin protein levels in HBMECs 2 days after siControl and siASL transfection. Samples were run on 2 separate gels, and alpha -Tubulin was used as the internal control (n = 3). (G) Protein abundance was quantitatively measured after normalization to alpha -Tubulin level. Bar graphs represent mean values while error bars represent the standard deviation. *P < 0.05, **P < 0.01, and ***P < 0.001. Student’s t test. A.U., arbitrary units. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37490345), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human VE‑Cadherin Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Simple Western

12.5 µg/mL
Sample: HUVEC human umbilical vein endothelial cells

Western Blot

0.25 µg/mL
Sample: HUVEC human umbilical vein endothelial cells

Reviewed Applications

Read 2 reviews rated 5 using AF938 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VE-Cadherin

Vascular endothelial (VE) - cadherin (VE-CAD), also called 7B4 and cadherin-5, is a member of the cadherin family of cell adhesion molecules. Cadherins are
calcium‑dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-Cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-CAD proteins share approximately 74% homology.

References

  1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
  2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  3. Overduin, M. et al. (1995) Science 267:386.
  4. Takeichi, M. (1991) Science 251:1451.
  5. Nose, A. et al. (1987) EMBO J. 6:3655.
  6. Carmeliet, P. et al. (1999) Cell 98:147.
  7. Gory-Faure, S. et al. (1999) Development 126:2093.

Long Name

Vascular Endothelium Cadherin

Alternate Names

Cadherin-5, CD144, CDH5, VECadherin

Entrez Gene IDs

1003 (Human); 12562 (Mouse)

Gene Symbol

CDH5

UniProt

P33151

Additional VE-Cadherin Products

Product Documents for Human VE‑Cadherin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human VE‑Cadherin Antibody

For research use only

Citations for Human VE‑Cadherin Antibody

Customer Reviews for Human VE‑Cadherin Antibody (2)

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Customer Images

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  • VE-Cadherin in human lungs
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Lung tissue
    Species: Human
    Verified Customer | Posted 11/19/2024
    VE-Cadherin expression in human lungs
    1:20 in milk-free antibody diluent. 1h of incubation in the primary antibodies.
    Human VE‑Cadherin Antibody AF938
  • Human VE-Cadherin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HMVEC human microvascular endothelial cells
    Species: Human
    Verified Customer | Posted 06/09/2021
    Human VE‑Cadherin Antibody AF938

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