Human VE-Cadherin Antibody

Catalog # Availability Size / Price Qty
AF938
AF938-SP
Human VE-Cadherin Antibody in Data
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Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (1)

Human VE-Cadherin Antibody Summary

Species Reactivity
Human
Specificity
Detects human VE-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse VE‑Cadherin is observed and less than 5% cross-reactivity with recombinant human E-Cadherin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human VE-Cadherin
Asp48-Gln593
Accession # P33151
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
HUVEC human umbilical vein endothelial cells
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human VE-Cadherin antibody by Western Blot. View Larger

Detection of Human VE‑Cadherin by Western Blot. Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human VE-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VE-Cadherin at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry VE-Cadherin antibody in HUVEC Cells by Immunocytochemistry (ICC). View Larger

VE‑Cadherin in HUVEC Cells. VE-Cadherin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human VE-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry VE-Cadherin antibody in HUVEC Cells by Immunocytochemistry (ICC). View Larger

VE‑Cadherin in HUVEC Cells. VE-Cadherin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human VE-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VE-Cadherin

Vascular endothelial (VE) - cadherin (VE-CAD), also called 7B4 and cadherin-5, is a member of the cadherin family of cell adhesion molecules. Cadherins are
calcium‑dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-Cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-CAD proteins share approximately 74% homology.

References
  1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
  2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  3. Overduin, M. et al. (1995) Science 267:386.
  4. Takeichi, M. (1991) Science 251:1451.
  5. Nose, A. et al. (1987) EMBO J. 6:3655.
  6. Carmeliet, P. et al. (1999) Cell 98:147.
  7. Gory-Faure, S. et al. (1999) Development 126:2093.
Long Name
Vascular Endothelium Cadherin
Entrez Gene IDs
1003 (Human); 12562 (Mouse)
Alternate Names
7B4 antigen; 7B4; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144 antigen; CD144; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VECadherin; VE-Cadherin

Product Datasheets

Citations for Human VE-Cadherin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Effect of Enzymatically Extracted Fucoidans on Angiogenesis and Osteogenesis in Primary Cell Culture Systems Mimicking Bone Tissue Environment
    Authors: J Ohmes, Y Xiao, F Wang, MD Mikkelsen, TT Nguyen, H Schmidt, A Seekamp, AS Meyer, S Fuchs
    Mar Drugs, 2020;18(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Identification of a combination of transcription factors that synergistically increases endothelial cell barrier resistance
    Authors: F Roudnicky, BK Kim, Y Lan, R Schmucki, V Küppers, K Christense, M Graf, C Patsch, M Burcin, CA Meyer, PD Westenskow, CA Cowan
    Sci Rep, 2020;10(1):3886.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Genetic disruption of the Blood Brain Barrier leads to protective barrier formation at the Glia Limitans
    Authors: P Mora, PL Hollier, S Guimbal, A Abelanet, A Diop, L Cornuault, T Couffinhal, S Horng, AP Gadeau, MA Renault, C Chapouly
    PLoS Biol, 2020;18(11):e3000946.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Mechanically activated ion channel PIEZO1 is required for lymphatic valve formation
    Authors: K Nonomura, V Lukacs, DT Sweet, LM Goddard, A Kanie, T Whitwam, SS Ranade, T Fujimori, ML Kahn, A Patapoutia
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. aPKC controls endothelial growth by modulating c-Myc via FoxO1 DNA-binding ability
    Authors: M Riddell, A Nakayama, T Hikita, F Mirzapours, T Kawamura, A Pasha, M Li, M Masuzawa, M Looso, T Steinbache, K Ebnet, M Potente, T Hirose, S Ohno, I Fleming, S Gattenlöhn, PP Aung, T Phung, O Yamasaki, T Yanagi, H Umemura, M Nakayama
    Nat Commun, 2018;9(1):5357.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Vascular CXCR4 expression promotes vessel sprouting and sensitivity to sorafenib treatment in hepatocellular carcinoma
    Authors: J Xu, J Liang, YM Meng, J Yan, XJ Yu, CQ Liu, L Xu, SM Zhuang, L Zheng
    Clin. Cancer Res, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. DeepCAGE transcriptomics identify HOXD10 as transcription factor regulating lymphatic endothelial responses to VEGF-C
    J Cell Sci, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. MicroRNA miR-27b rescues bone marrow-derived angiogenic cell function and accelerates wound healing in type 2 diabetes mellitus.
    Authors: Wang J, Tao J, Chen D, Cai J, Irani K, Wang Q, Yuan H, Chen A
    Arterioscler Thromb Vasc Biol, 2014;34(0):99.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors.
    Authors: Niwa A, Heike T, Umeda K
    PLoS ONE, 2011;6(7):e22261.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Signaling hierarchy regulating human endothelial cell development.
    Authors: Kelly MA, Hirschi KK
    Arterioscler. Thromb. Vasc. Biol., 2009;29(5):718-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Human VE-Cadherin Antibody
By Anonymous on 06/09/2021
Application: ICC/IF Sample Tested: HMVEC human microvascular endothelial cells Species: Human