Human VISTA/B7-H5/PD-1H Antibody

Catalog # Availability Size / Price Qty
MAB71266-100
MAB71266-SP
Human VISTA/B7-H5/PD-1H Antibody in Blockade of Receptor-ligand Interaction
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Human VISTA/B7-H5/PD-1H Antibody Summary

Species Reactivity
Human
Specificity
Detects human VISTA/B7-H5/PD-1H in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 1011478
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human VISTA/B7-H5/PD-1H
Phe33-Ala194
Accession # Q9H7M9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Blockade of Receptor-ligand Interaction
In a functional ELISA, 2-12 µg/mL of this antibody will block 50% of the binding of 5 μg/mL of Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera (Catalog # 7126-B7) to immobilized Recombinant Human VSIG3 Fc Chimera (Catalog # 9229-VS) coated at 2 µg/mL (100 µL/well). At 25 µg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Blockade of Receptor-ligand Interaction VISTA/B7-H5/PD-1H Binding to VSIG3 Blocked by Human VISTA/B7-H5/PD-1H Antibody. View Larger

VISTA/B7-H5/PD-1H Binding to VSIG3 Blocked by Human VISTA/B7-H5/PD-1H Antibody. In a functional ELISA, Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera (Catalog # 7126-B7) binds to immobilized Recombinant Human VSIG3 Fc Chimera (Catalog # 9229-VS) coated at 2 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Mouse Anti-Human VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB71265). At 25 µg/mL, this antibody will block >90% of the binding.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VISTA/B7-H5/PD-1H

Platelet Receptor Gi24, also known as VISTA (V-domain Ig suppressor of T cell activation), B7-H5, B7H5, Dies1 (Differentiation of ESC-1), SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162 aa extracellular region with one V-type Ig-like domain and a 96 aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. Over aa 33-194, human Gi24 shares 70% and 67% aa identity with mouse and rat Gi24, respectively.

Entrez Gene IDs
64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey)
Alternate Names
4632428N05Rik; B7H5; B7-H5; C10orf54; chromosome 10 open reading frame 54; Dies1; Gi24; PD1H; PD-1H; platelet receptor Gi24; PP2135; SISP1; stress induced secreted protein 1; VISTA; VSIR

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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