Human Vitronectin Antibody

Catalog # Availability Size / Price Qty
AF2349
AF2349-SP
Human Vitronectin Antibody in Western Blot
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Human Vitronectin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Vitronectin in direct ELISAs and Western blot.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Human plasma-derived human Vitronectin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunohistochemistry
3-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Vitronectin by Western Blot. View Larger

Detection of Human Vitronectin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Vitronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2349) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Vitronectin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Vitronectin in Human Bladder. View Larger

Vitronectin in Human Bladder. Vitronectin was detected in immersion fixed paraffin-embedded sections of human bladder using Goat Anti-Human Vitronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2349) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to extracellular matrix. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection  of Human Vitronectin by Simple WesternTM. View Larger

Detection of Human Vitronectin by Simple WesternTM. Simple Western lane view shows human serum, loaded at a 1:300 dilution. A specific band was detected for Vitronectin at approximately 76 kDa (as indicated) using 1 µg/mL of Goat Anti-Human Vitronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2349) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vitronectin

VN/Vitronectin (vitro-nectin: Latin for "glass" and "to bind to", respectively) is a 65-78 kDa circulating glycoprotein that was initially named for to its ability to 1) bind to glass beads, and 2) attach to cell membranes. Also known as somatomedin-B and Serum Spreading Factor, vitronectin is a matricellular protein that belongs to a group of RGD-type adhesive molecules. It is secreted by hepatocytes and circulates as a monomer/homodimer. Upon binding to ECM or PIA-1, it oligomerizes and becomes active. VN acts as a promoter of cell migration and ECM remodeling. It does so by binding to HSPG in the ECM which exposes binding sites for integrins and uPAR on cells. In this context, VN allows for the formation of a uPAR:soluble uPAR:uPA complex that results in the proteolysis of pericellular matrix and subsequent cell migration. Vitronectin also serves as a receptor for circulating PAI-1. When bound, VN forms oligomers that facilitate subsequent binding to vimentin, a cellular intermediate filament. The vimentin:VN:PAI-1 complex is active, and blocks the enzymatic degradation of vascular clots. Human secreted vitronectin is a 459 amino acid (aa) glycoprotein. It can exist as either a 77-78 kDa monomer, or an internal disulfide-linked 65 kDa:10 kDa heterodimer that arises from proteolytic cleavage between Ala398Thr399. VN has multiple domains, including a somatomedin B domain that binds uPAR and PAI-1 (aa 1-40), an RGD motif (aa 64-66) and a heparin-binding region (aa 340-380). VN is sulfated, N- and O-glycosylated, and phosphorylated on at least five Thr/Ser based sites. Over aa 20-29 (i.e.‑DQESCKGRCT), human and mouse VN are identical in their amino acid sequences.

Entrez Gene IDs
7448 (Human); 22370 (Mouse); 507525 (Bovine)
Alternate Names
Complement S-protein; epibolin; Serum Spreading Factor; Serum-spreading factor; Somatomedin B; S-protein; V75; vitronectin (serum spreading factor, somatomedin B, complement S-protein); Vitronectin; VN; VNT; VTN

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Vitronectin Antibody

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Human Vitronectin Antibody
By Anonymous on 09/30/2019
Application: ELISA Sample Tested: Recombinant protein Species: Human

Paired with MAB2349