VSIG3 (V-set and Ig domain-containing protein 3; also BT-IgSF and IGSF11) is a 52 kDa brain and testis-specific protein that belongs to the IGSF11 family of proteins. It is expressed by neurons, astrocytes and oligodendroglia. VSIG3 is an adhesion molecule that forms Ca-independent homophilic interactions in trans. Human VSIG3 is 413 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 219 aa extracellular domain (ECD). The ECD contains one V-type (aa 23‑136) and one C2-type Ig-like domain (aa 144‑234). Over aa 23‑245, human VSIG3 is 94% aa identical to mouse VSIG3. Two potential splice variants exist in human. Both exhibit a 16 aa substitution for the first 17 aa of the signal sequence, and one contains an additional single Ala substitution for aa 211‑235.
Key Product Details
Species Reactivity
Human
Applications
Blockade of Receptor-ligand Interaction
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 774206
Loading...
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human VSIG3
Met1-Gly245
Accession # Q5DX21
Met1-Gly245
Accession # Q5DX21
Specificity
Detects human VSIG3 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human VSIG3 Antibody
VSIG3 Binding to VISTA/B7-H5/PD-1H by Human VISG3 Antibody.
In a functional ELISA, 0.2-2 µg/mL of this antibody will block 50% of the binding of 1 µg/mL of Recombinant Human VSIG3 Fc Chimera (Catalog # 9229-VS) to immobilized Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera (Catalog # 7126-B7) coated at 1 µg/mL (100 µL/well). At 10 µg/mL, this antibody will block >90% of the binding.Applications for Human VSIG3 Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.2-2 µg/mL of this antibody will block 50% of the binding of 1 μg/mL of Recombinant Human VSIG3 Fc Chimera (Catalog # 9229-VS ) to immobilized Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera (Catalog # 7126-B7) coated at 1 µg/mL (100 µL/well). At 10 µg/mL, this antibody will block >90% of the binding.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: VSIG3
Long Name
V-Set and Immunoglobulin Domain Containing 3
Alternate Names
BTIGSF, CXADRL1, IGSF11
Gene Symbol
IGSF11
UniProt
Additional VSIG3 Products
Product Documents for Human VSIG3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human VSIG3 Antibody
For research use only
Related Research Areas
Customer Reviews for Human VSIG3 Antibody
There are currently no reviews for this product. Be the first to review Human VSIG3 Antibody and earn rewards!
Have you used Human VSIG3 Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Loading...