Human Wnt-9a Antibody

Catalog # Availability Size / Price Qty
Wnt‑9a in Human Breast Cancer Tissue.
1 Image
Product Details
Supplemental Products
Reviews (2)

Human Wnt-9a Antibody Summary

Species Reactivity
Detects recombinant human Wnt-9a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Wnt-2, -7a, -7b, recombinant mouse Wnt-1, -2b, -3a, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, or -16 is observed
Monoclonal Mouse IgG2A Clone # 326707
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Wnt‑9a
Pro48-Glu92 & Arg231-Phe304
Accession # O14904.2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human Wnt‑9a
under non-reducing conditions only
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Wnt‑9a in Human Breast Cancer Tissue. View Larger

Wnt‑9a in Human Breast Cancer Tissue. Wnt‑9a was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Mouse Anti-Human Wnt‑9a Monoclonal Antibody (Catalog # MAB31571) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-9a

Wnt-9a (formerly Wnt14) is a member of the Wnt family, which are ligands for frizzled family receptors. Wnt-9a is a secreted glycoprotein expressed in skeletal muscle and heart as well as in B-CLL (B cell chronic lymphocytic leukemia) and gastric, pancreatic and breast cancer cells. Human Wnt-9a shares 98% aa sequence identity with mouse and rat Wnt-9a.

Long Name
Wingless-type MMTV Integration Site Family, Member 9A
Entrez Gene IDs
7483 (Human); 216795 (Mouse)
Alternate Names
MGC138165; MGC141991; Protein Wnt-14; wingless-type MMTV integration site family, member 14; wingless-type MMTV integration site family, member 9A; Wnt-14; WNT14protein Wnt-9a; Wnt9a; Wnt-9a

Product Datasheets


No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control


Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

Reviews for Human Wnt-9a Antibody

Average Rating: 3.5 (Based on 2 Reviews)

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Human Wnt-9a Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Wnt-9a Antibody
By Anonymous on 02/21/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human