Detects recombinant human Wnt-9a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Wnt-2, -7a, -7b, recombinant mouse Wnt-1, -2b, -3a, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, or -16 is observed
Monoclonal Mouse IgG2A Clone # 326707
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Wnt‑9a Pro48-Glu92 & Arg231-Phe304 Accession # O14904.2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Wnt‑9a in Human Breast Cancer Tissue.
Wnt‑9a was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Mouse Anti-Human Wnt‑9a Monoclonal Antibody (Catalog # MAB31571) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Wnt-9a (formerly Wnt14) is a member of the Wnt family, which are
ligands for frizzled family receptors. Wnt-9a is a secreted
glycoprotein expressed in skeletal muscle and heart as well as in B-CLL (B cell chronic lymphocytic leukemia)
and gastric, pancreatic and breast cancer cells. Human Wnt-9a shares 98% aa sequence identity with mouse and rat Wnt-9a.
Wingless-type MMTV Integration Site Family, Member 9A
Entrez Gene IDs:
7483 (Human); 216795 (Mouse)
MGC138165; MGC141991; Protein Wnt-14; wingless-type MMTV integration site family, member 14; wingless-type MMTV integration site family, member 9A; Wnt-14; WNT14protein Wnt-9a; Wnt9a; Wnt-9a
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