Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 326707
View all Wnt-9a Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Wnt‑9a
Pro48-Glu92 & Arg231-Phe304
Accession # O14904.2

Specificity

Detects recombinant human Wnt-9a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Wnt-2, -7a, -7b, recombinant mouse Wnt-1, -2b, -3a, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, or -16 is observed

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Wnt‑9a Antibody

Wnt‑9a antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

Wnt‑9a in Human Breast Cancer Tissue.

Wnt‑9a was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Mouse Anti-Human Wnt‑9a Monoclonal Antibody (Catalog # MAB31571) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Wnt‑9a Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

1 µg/mL
Sample: Recombinant Human Wnt‑9a
under non-reducing conditions only

Reviewed Applications

Read 3 reviews rated 4 using MAB31571 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-9a

Wnt-9a (formerly Wnt14) is a member of the Wnt family, which are ligands for frizzled family receptors. Wnt-9a is a secreted glycoprotein expressed in skeletal muscle and heart as well as in B-CLL (B cell chronic lymphocytic leukemia) and gastric, pancreatic and breast cancer cells. Human Wnt-9a shares 98% aa sequence identity with mouse and rat Wnt-9a.

Long Name

Wingless-type MMTV Integration Site Family, Member 9A

Alternate Names

Wnt-14, Wnt9a

Entrez Gene IDs

7483 (Human); 216795 (Mouse)

Gene Symbol

WNT9A

UniProt

O14904.2

Additional Wnt-9a Products

Product Documents for Human Wnt‑9a Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Wnt‑9a Antibody

For research use only

Related Research Areas

Wnt Ligands (Wnts)

Customer Reviews for Human Wnt‑9a Antibody (3)

4 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
33%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • Human Wnt-9a Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 01/23/2022
    Human Wnt‑9a Antibody MAB31571
  • Human Wnt-9a Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human Wnt‑9a Antibody MAB31571
  • Human Wnt-9a Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/21/2019

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Protocols

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FAQs

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