Human Wnt-9a Antibody Summary
Pro48-Glu92 & Arg231-Phe304
Accession # O14904.2
under non-reducing conditions only
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Wnt‑9a in Human Breast Cancer Tissue. Wnt‑9a was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Mouse Anti-Human Wnt‑9a Monoclonal Antibody (Catalog # MAB31571) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Wnt-9a (formerly Wnt14) is a member of the Wnt family, which are ligands for frizzled family receptors. Wnt-9a is a secreted glycoprotein expressed in skeletal muscle and heart as well as in B-CLL (B cell chronic lymphocytic leukemia) and gastric, pancreatic and breast cancer cells. Human Wnt-9a shares 98% aa sequence identity with mouse and rat Wnt-9a.
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Cell and Tissue Staining Kits
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