Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Wnt-9a Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Wnt‑9a
Pro48-Glu92 & Arg231-Phe304
Accession # O14904.2

Specificity

Detects human Wnt-9a in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) Wnt‑9b is observed and less than 1% cross-reactivity with rmWnt-1 and rmWnt-4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Wnt‑9a Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Western Blot

0.1 µg/mL
Sample: Recombinant Human Wnt‑9a

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Wnt-9a

Wnt-9a, previously known as Wnt-14, is a secreted glycoprotein belonging to the Wnt family of signaling molecules that are important in development. Within the sequences used as immunogens, human Wnt-9a shares 97% amino acid sequence identity with the mouse or rat protein.

Long Name

Wingless-type MMTV Integration Site Family, Member 9A

Alternate Names

Wnt-14, Wnt9a

Entrez Gene IDs

7483 (Human); 216795 (Mouse)

Gene Symbol

WNT9A

UniProt

O14904.2

Additional Wnt-9a Products

Product Documents for Human Wnt‑9a Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Wnt‑9a Antibody

For research use only

Related Research Areas

Wnt Ligands (Wnts)

Citations for Human Wnt‑9a Antibody

Customer Reviews for Human Wnt‑9a Antibody

There are currently no reviews for this product. Be the first to review Human Wnt‑9a Antibody and earn rewards!

Have you used Human Wnt‑9a Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies