Human XCL1/Lymphotactin Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Ddetects human XCL1/Lymphotactin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Lymphotactin is observed, and less than 1% cross-reactivity with recombinant human (rh) Eotaxin-3, rhHCC-1, and rhMCP-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human XCL1/Lymphotactin
    Val22-Gly114
    Accession # P47992
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human XCL1/Lymphotactin (Catalog # 695-LT)
  • Neutralization
    Measured by its ability to neutralize XCL1/Lymphotactin-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human XCR1. The Neutralization Dose (ND50) is typically 10-30 µg/mL in the presence of 0.5 µg/mL Recombinant Human XCL1/Lymphotactin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by XCL1/Lymphotactin and Neutralization by Human XCL1/Lymphotactin Antibody.

Recombinant Human XCL1/
Lymphotactin (Catalog # 695-LT) chemoattracts the BaF3 mouse pro-B cell line transfected with human XCR1 in a dosdependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis  elicited by Recombinant Human XCL1/
Lymphotactin (0.5 μg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human XCL1/
Lymphotactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF695). The ND50 is typically 10-30 µg/mL.

 

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: XCL1/Lymphotactin

Human lymphotactin (Lptn)/XCL1 (also named human SCM-1 alpha and ATAC) and its mouse homologue belong to the C or gamma  subfamily of chemokines. The C chemokines lack two (the 1st and 3rd ) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Human lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is abundant in some activated T cells such as activated CD8+ T cells and other class I MHC restricted T cells. Lptn expression is absent in CD4+ T cells. Human and mouse Lptn share approximately 60% amino acid sequence homology. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse. Recombinant human lymphotactin has been shown to have chemotactic activity for lymphocytes and NK cells. The orphan receptor GPR5 has been reported to be the specific receptor for Lptn.

  • References:
    1. Kennedy, J. et al. (1995) J. Immunol. 155:203.
    2. Zlotnik, A. et al. (1999) Critical Reviews in Immunol. 19:1.
  • Long Name:
    Chemokine [C Motif] Ligand 1
  • Entrez Gene IDs:
    6375 (Human); 16963 (Mouse)
  • Alternate Names:
    ATAC; ATACSmall-inducible cytokine C1; C motif chemokine 1; chemokine (C motif) ligand 1; Cytokine SCM-1; LPTNSCM-1-alpha; LTNXC chemokine ligand 1; Lymphotactin; lymphotaxin; SCM-1 alpha; SCM-1; SCM1A; SCM-1a; SCYC1SCM1; single cysteine motif 1a; small inducible cytokine subfamily C, member 1 (lymphotactin); XCL1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Applications
Sample Type
  1. The CD8-derived chemokine XCL1/lymphotactin is a conformation-dependent, broad-spectrum inhibitor of HIV-1.
    Authors: Guzzo C, Fox J, Lin Y, Miao H, Cimbro R, Volkman B, Fauci A, Lusso P
    PLoS Pathog, 2013;9(12):e1003852.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Co-immunoprecipitation
  2. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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