Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Virus - HIV-1

Applications

Validated:

Western Blot, Neutralization

Cited:

Flow Cytometry, Capture Assay, Co-Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all XCL1/Lymphotactin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human XCL1/Lymphotactin
Val22-Gly114
Accession # P47992

Specificity

Ddetects human XCL1/Lymphotactin in direct ELISAs and Western blots. 

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human XCL1/Lymphotactin Antibody

Chemotaxis Induced by XCL1/Lymphotactin and Neutralization by Human XCL1/Lymphotactin Antibody.

Chemotaxis Induced by XCL1/Lymphotactin and Neutralization by Human XCL1/Lymphotactin Antibody.

Recombinant Human XCL1/ Lymphotactin (695-LT) chemoattracts the BaF3 mouse pro-B cell line transfected with human XCR1 in a dose dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human XCL1/ Lymphotactin (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human XCL1/ Lymphotactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF695). The ND50 is typically 3.00-30.0 µg/mL.

Applications for Human XCL1/Lymphotactin Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human XCL1/Lymphotactin (Catalog # 695-LT)

Neutralization

Measured by its ability to neutralize XCL1/Lymphotactin-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human XCR1. The Neutralization Dose (ND50) is typically 3.00-30.0 µg/mL in the presence of 0.5 µg/mL Recombinant Human XCL1/Lymphotactin.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: XCL1/Lymphotactin

Human lymphotactin (Lptn)/XCL1 (also named human SCM-1 alpha and ATAC) and its mouse homologue belong to the C or gamma  subfamily of chemokines. The C chemokines lack two (the 1st and 3rd ) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Human lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is abundant in some activated T cells such as activated CD8+ T cells and other class I MHC restricted T cells. Lptn expression is absent in CD4+ T cells. Human and mouse Lptn share approximately 60% amino acid sequence homology. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse. Recombinant human lymphotactin has been shown to have chemotactic activity for lymphocytes and NK cells. The orphan receptor GPR5 has been reported to be the specific receptor for Lptn.

References

  1. Kennedy, J. et al. (1995) J. Immunol. 155:203.
  2. Zlotnik, A. et al. (1999) Critical Reviews in Immunol. 19:1.

Long Name

Chemokine [C Motif] Ligand 1

Alternate Names

ATAC, Lymphotactin, SCM-1 alpha

Entrez Gene IDs

6375 (Human); 16963 (Mouse)

Gene Symbol

XCL1

UniProt

P47992

Additional XCL1/Lymphotactin Products

Product Documents for Human XCL1/Lymphotactin Antibody

Certificate of Analysis

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Product Specific Notices for Human XCL1/Lymphotactin Antibody

For research use only

Related Research Areas

Chemokines and Receptors

Citations for Human XCL1/Lymphotactin Antibody

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