Human ZAP70 Antibody

Catalog # Availability Size / Price Qty
Detection of Human ZAP70 by Western Blot.
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Product Details
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Human ZAP70 Antibody Summary

Species Reactivity
Detects human ZAP70 in direct ELISAs and Western blots.
Monoclonal Mouse IgG2B Clone # 521626
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human ZAP70
Accession # P43403
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ZAP70 antibody by Western Blot. View Larger

Detection of Human ZAP70 by Western Blot. Western blot shows lysates of CEM human T-lymphoblastoid cell line, MOLT-4 human acute lymphoblastic leukemia cell line, Jurkat human acute T cell leukemia cell line, and Raji human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human ZAP70 Monoclonal Antibody (Catalog # MAB3709) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ZAP70 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZAP70

ZAP70 (zeta-chain (TCR) associated protein kinase 70 kDa), expressed primarily in T and NK cells, is a Syk family cytosolic protein tyrosine kinase that consists of two N-terminal SH2 domains and a C-terminal tyrosine kinase domain. Upon T cell receptor activation and phosphorylation of TCR ITAMs by Src family kinases, ZAP70 is recruited to phosphorylated ITAM sequences and subsequently phosphorylated on several tyrosine residues. ZAP70 has been implicated in several immune disorders. An autosomal recessive form of SCID in humans has been attributed to a homozygous mutation in the kinase domain of ZAP70. ZAP70 expression also defines an aggressive subset of chronic lymphocytic leukemia.

Long Name
Zeta-chain [TCR] Associated Protein Kinase 70 kDa
Entrez Gene IDs
7535 (Human); 22637 (Mouse); 301348 (Rat)
Alternate Names
70 kDa zeta-associated protein; EC 2.7.10; EC; mrtle; mur; SRK; SRKFLJ17679; STD; STDFLJ17670; Syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; TZK; ZAP70; ZAP-70; zeta-chain (TCR) associated protein kinase (70 kD); zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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