Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Carbonic Anhydrase IX/CA9 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase IX/CA9
Gln32-Asp390
Accession # Q8VHB5

Specificity

Detects mouse Carbonic Anhydrase IX/CA9 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) CA9 is observed, 5% cross-reactivity with recombinant mouse (rm) CA14 is observed, and less than1% cross‑reactivity with rhCA1, -2, -3, -8, -10, -13, rmCA4, and -12 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Carbonic Anhydrase IX/CA9 Antibody

Carbonic Anhydrase IX antibody in Mouse Colon by Immunohistochemistry (IHC-Fr).

Carbonic Anhydrase IX in Mouse Colon.

Carbonic Anhydrase IX was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse Carbonic Anhydrase IX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2344) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the brush border of epithelial cells in villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Carbonic Anhydrase IX/CA9 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse colon

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Carbonic Anhydrase IX (Catalog # 2344-CA), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Carbonic Anhydrase IX (Catalog # 2344-CA)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carbonic Anhydrase IX/CA9

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20-25, 2003, in Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Carbonic Anhydrase IX (CA9), also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (2, 3). Recombinant mouse CA9 corresponds to the extracellular portion of mouse CA9.

References

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
  3. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.

Alternate Names

CA9, G250, MN, RCC

Entrez Gene IDs

768 (Human); 230099 (Mouse)

Gene Symbol

CA9

UniProt

Q8VHB5

Additional Carbonic Anhydrase IX/CA9 Products

Product Documents for Mouse Carbonic Anhydrase IX/CA9 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Carbonic Anhydrase IX/CA9 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Mouse Carbonic Anhydrase IX/CA9 Antibody

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Protocols

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