Mouse Carbonic Anhydrase IX/CA9 Antibody

(10 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Carbonic Anhydrase IX/CA9 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) CA9 is observed, 5% cross-reactivity with recombinant mouse (rm) CA14 is observed, and less than
    1% cross‑reactivity with rhCA1, -2, -3, -8, -10, -13, rmCA4, and -12 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase IX/CA9
    Gln32-Asp390
    Accession # Q8VHB5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Carbonic Anhydrase IX in Mouse Colon. Carbonic Anhydrase IX was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse Carbonic Anhydrase IX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2344) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the brush border of epithelial cells in villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase IX/CA9

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20-25, 2003, in Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Carbonic Anhydrase IX (CA9), also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (2, 3). Recombinant mouse CA9 corresponds to the extracellular portion of mouse CA9.

  • References:
    1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
    2. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
    3. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.
  • Entrez Gene IDs:
    768 (Human); 230099 (Mouse)
  • Alternate Names:
    CA9; CA-IX; CAIXcarbonic anhydrase 9; Carbonate dehydratase IX; Carbonic Anhydrase IX; carbonic anhydrase IXpMW1; carbonic dehydratase; EC 4.2.1.1; G250; Membrane antigen MN; MN; MNRCC-associated antigen G250; P54/58N; RCC; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. CD44 Interacts with HIF-2? to Modulate the Hypoxic Phenotype of Perinecrotic and Perivascular Glioma Cells
    Authors: E Johansson, ES Grassi, V Pantazopou, B Tong, D Lindgren, TJ Berg, EJ Pietras, H Axelson, A Pietras
    Cell Rep, 2017;20(7):1641-1653.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Simultaneous targeted activation of Notch1 and Vhl-disruption in the kidney proximal epithelial tubular cells in mice
    Sci Rep, 2016;6(0):30739.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Bone marrow-derived mesenchymal stem cells drive lymphangiogenesis.
    Authors: Maertens L, Erpicum C, Detry B, Blacher S, Lenoir B, Carnet O, Pequeux C, Cataldo D, Lecomte J, Paupert J, Noel A
    PLoS ONE, 2014;9(9):e106976.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. Characterization of the adverse effects of nicotine on placental development: in vivo and in vitro studies.
    Authors: Holloway, A C, Salomon, A, Soares, M J, Garnier, V, Raha, S, Sergent, F, Nicholson, C J, Feige, J J, Benharouga, M, Alfaidy, N
    Am J Physiol Endocrinol Metab, 2014;306(4):E443-56.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
  5. Expression patterns and subcellular localization of carbonic anhydrases are developmentally regulated during tooth formation.
    Authors: Reibring C, El Shahawy M, Hallberg K, Kannius-Janson M, Nilsson J, Parkkila S, Sly W, Waheed A, Linde A, Gritli-Linde A
    PLoS ONE, 2014;9(5):e96007.
  6. Cooperation between Pik3ca and p53 mutations in mouse mammary tumor formation.
    Authors: Adams JR, Xu K, Liu JC, Agamez NM, Loch AJ, Wong RG, Wang W, Wright KL, Lane TF, Zacksenhaus E, Egan SE
    Cancer Res., 2011;71(7):2706-17.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  7. Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
    Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
    Nature, 2011;475(7355):226-30.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  8. VHL loss actuates a HIF-independent senescence programme mediated by Rb and p400.
    Authors: Young AP, Schlisio S, Minamishima YA, Zhang Q, Li L, Grisanzio C, Signoretti S, Kaelin WG
    Nat. Cell Biol., 2008;10(3):361-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  9. CO2 chemosensing in rat oesophagus.
    Authors: Akiba Y, Mizumori M, Kuo M, Ham M, Guth PH, Engel E, Kaunitz JD
    Gut, 2008;57(12):1654-64.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  10. Carbonic anhydrases and mucosal vanilloid receptors help mediate the hyperemic response to luminal CO2 in rat duodenum.
    Authors: Akiba Y, Ghayouri S, Takeuchi T, Mizumori M, Guth PH, Engel E, Swenson ER, Kaunitz JD
    Gastroenterology, 2006;131(1):142-52.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
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