|Detection of Recombinant Mouse CCL2/JE/MCP‑1 by Western Blot. Western blot shows 25 ng of Recombinant Mouse CCL2/JE/MCP‑1 (Catalog # 479-JE), Recombinant Human CCL2/JE/MCP‑1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP‑1 (Catalog # 3144-JE), Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME), Recombinant Mouse CCL12/MCP‑5 (Catalog # 428-P5), and Recombinant Mouse CCL7/MCP‑3/MARC (Catalog # 456-MC). PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse CCL2/JE/MCP‑1 Polyclonal Antibody (Catalog # AB-479-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/MCP‑1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.|
|Chemotaxis Induced by CCL2/JE/MCP‑1 and Neutralization by Mouse CCL2/JE Antibody. Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL2/JE/MCP‑1 Polyclonal Antibody (Catalog # AB-479-NA). The ND50 is typically 10-30 µg/mL.|
Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.
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