Mouse CD4 Antibody

Detection of CD4 by Flow Cytometry

Chronic wounds result in a delayed Th2 response that persists as the wound heals. (A) Representative high-power immunohistochemistry (zoom-out 2×; zoom-in 40×) and quantification of (B) CD4+ T-helper cells in the distal peri-wound regions (n = 5, 2–3 HPF/animal, p < 0.01 at week 2, p < 0.0001 at week 4, and p < 0.001 at week 6 post-operation) (C) Representative high-power immunohistochemistry (zoom-out 2×; zoom-in 40×) and (D) quantification of Gata3+ Th2 cells in the distal peri-wound regions (n = 5, 2–3 HPF/animal, p < 0.01 at week 2, p < 0.0001 at week 4, and p < 0.001 at week 6 post-operation). (E) Representative flow cytometry plots displaying CD45+ on the y axis and CD4+ cells (T cells) on the x axis. (F) Quantification of CD4+ T cells as a percentage of CD45+ cells (p < 0.05 between weeks 2 and 4 post-operation). (G) Representative flow cytometry plots displaying CCR4+ on the y axis and CCR8+ on the x axis to identify Th2 cells. (H) Quantification of Th2+ T cells as a percentage of CD4+ T cells (p < 0.05 between weeks 2 and 4 post-operation). Red square represents gating for CD4+ & CD45+ double positive cells. As for colors, there are only 3 as mentioned in the figure: CD4 (green) LYVE (red) or Gata3 (red) DAPI (blue) and when they colocalize they may appear in different shades * denotes p < 0.05, ** denotes p < 0.01, # denotes p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40426856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of CD4 by Flow Cytometry

Inhibition of Th2 cytokines results in decreased peri-wound Th2 cell infiltration. (A) Representative high-power immunohistochemistry (zoom-out 2×; zoom-in 40×) and quantification of (B) CD4+ T-helper cells in the distal peri-wound regions (n = 5, 2–3 HPF/animal; p < 0.01). (C) Representative high-power immunohistochemistry (zoom-out 2×; zoom-in 40×) and quantification of (D) Gata3+ Th2 cells in the distal peri-wound regions (n = 5, 2–3 HPF/animal; p < 0.01 when comparing between isotype and IL-4 mAB and p < 0.0001 when comparing between isotype and IL-13 mAb-treated animals). (E) Representative flow cytometry plots displaying CD45+ on the y axis and CD4+ cells (T cells) on the x axis. (F) Quantification of CD4+ T cells as a percentage of CD45+ cells (p < 0.01). (G) Representative flow cytometry plots displaying CCR4+ on the y axis and CCR8+ on the x axis to identify Th2 cells. (H) Quantification of Th2+ T cells as a percentage of CD4+ T cells (p < 0.01). As for colors, there are only 3 as mentioned in the figure: CD4 (green) Gata3 (red) DAPI (blue) and when they colocalize they may appear in different shades. ** denotes p < 0.01, **** denotes p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40426856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of CD4 by Immunohistochemistry

Asthma reduces T cell induction of Ccl5 in microglia.a Schematic representation of the mouse Nf1OPG neuron-immune-cancer cell axis in the setting of asthma. b No difference in optic nerve Mdk RNA expression was observed between OVA- (n = 3) or HDM- (n = 3) treated mice and controls (n = 6). Bar graphs represent the means ± SEM of independent biological samples. One-way ANOVA with Bonferroni post hoc correction. c Ccl4 levels are similarly induced with increasing midkine concentrations (0–100 ng/ml) in CD3+ T cells from OVA- and PBS-treated mice. Data are presented as the means ± SEM of n = 3 independent biological samples. One-way ANOVA with Bonferroni post hoc correction. Data are presented as the means ± SEM. d, e Similarly increased CD8+, relative to CD4+, T cell content was detected in the optic nerves of Nf1OPG mice treated with PBS (n = 8), OVA (n = 8) or HDM (n = 8). One-way ANOVA with Bonferroni post hoc correction. Activated T cell medium from (f) OVA- (n = 4) and g HDM- (n = 4) treated mice induced lower levels of microglial Ccl5 relative to PBS-treated Nf1OPG mice. Two-tailed Student’s t test. Similar reductions of Ccl5 expression were observed in CD4+ and CD8+ T cells from (h) OVA- (n = 3) and (i) HDM- (n = 3) treated mice relative to PBS-treated controls (n = 3). One-way ANOVA with Bonferroni post hoc correction. Data are presented as the means ± SEM. d Scale bars, 40 µm. From left to right in each panel: b ns; c ns; ns; ns; ns; eP < 0.0001, P = 0.0022, P = 0.0011; fP = 0.0008; gP = 0.0015; hP = 0.0186, P = 0.0054; iP = 0.0224, P = 0.0007. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34880260), licensed under a CC-BY license. Not internally tested by R&D Systems.