Mouse CD4 Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (2)
Supplemental Products

Mouse CD4 Antibody Summary

Species Reactivity
Detects mouse CD4 in direct ELISAs and Western blots. In these formats, less than 1% cross‑reactivity with recombinant human CD4, recombinant feline CD4, and recombinant canine CD4 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse CD4
Accession # P06332
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse CD4

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4 (Cluster of Differentiation #4; also L3T4) is a 55 kDa member of the Ig Superfamily of molecules.  It is expressed on a variety of hematopoietic cell types, including immature thymocytes, a number of variable phenotype single positive T cells (i.e.- CD25+; CD39+; Th1, 2 and 17 type cells), stimulated CD8+ T cells, pluripotent bone marrow stem cells, and NKT cells.  Although CD4 is a standard phenotype marker, functionally, the molecule is known to bind to APC MHC class II, and to associate with adjacent CD3 molecules to link p56lck with the lymphocyte TCR:CD3 complex.  Mature mouse CD4 is a 431 amino acid (aa) type I transmembrane glycoprotein.  It contains a 368 aa extracellular region (aa 27-394) plus a 40 aa cytoplasmic domain (aa 418-457).  The extracellular region contains one V-type (aa 27-128) and three C2-type Ig-like domains (aa 129-374).  There is one alternative start site at Met241 that is utilized in brain.  Over aa 27-394, mouse CD4 shares 53% and 72% aa identity with human and rat CD4, respectively.        

Entrez Gene IDs
920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)
Alternate Names
CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Citations for Mouse CD4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Determinants in the beta and delta subunit cytoplasmic loop regulate Golgi trafficking and surface expression of the muscle acetylcholine receptor.
    Authors: Rudell J, Borges L, Rudell J, Beck K, Ferns M
    J Biol Chem, 2014;289(1):203-14.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. Timing of estrogen replacement influences atherosclerosis progression and plaque leukocyte populations in ApoE-/- mice.
    Authors: Cann JA, Register TC, Adams MR, St Clair RW, Espeland MA, Williams JK
    Atherosclerosis, 2008;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr


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