Mouse CHL-1/L1CAM-2 Antibody

(8 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CHL‑1/L1CAM‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human CHL-1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CHL‑1/L1CAM‑2
    Ala25-Gln1043 (Leu227-Gln242 del, Ala243Ser)
    Accession # BAC30699
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • Simple Western
    2.5 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Perfusion fixed frozen sections of mouse brain (cortex)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse CHL‑1/L1CAM‑2 by Western Blot. Western blot shows lysates of mouse brain stem tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse CHL‑1/L1CAM‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2147) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CHL‑1/L1CAM‑2 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse CHL‑1/L1CAM‑2 by Simple WesternTM. Simple Western lane view shows lysates of mouse brain (cortex) tissue and mouse brain stem tissue, loaded at 0.2 mg/mL. A specific band was detected for CHL‑1/L1CAM‑2 at approximately 196-201 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse CHL‑1/L1CAM‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2147) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CHL-1/L1CAM-2

Close homolog of L1 (CHL-1), also known as cell adhesion L1-like (CALL) and L1 cell adhesion molecule 2 (L1CAM-2), belongs to the L1 subfamily of immunoglobulin (Ig) superfamily cell adhesion molecules, which also includes L1, neurofascin and NgCAM-related cell adhesion molecule (NrCAM) (1‑3). These molecules are type I transmembrane proteins that have 6 Ig-like domains and 4‑5 fibronectin type III-like (FNIII) domains in their extracellular regions. They also share a highly conserved cytoplasmic region of approximately 110 amino acid (aa) residues containing an ankyrin-binding site. CHL-1 is expressed as a highly glycosylated 185 kDa transmembrane protein by subpopulations of neurons and glia of the central and peripheral nervous system (4, 5). Ectodomain shedding via the metalloprotease-disintegrin ADAM8 releases 165 kDa and 125 kDa soluble CHL-1 fragments, which can diffuse away to function at distant sites (6). CHL-1 is not capable of homotypic interactions, but an extracellular binding partner of CHL-1 has not been identified (4). Human CHL1 has been mapped to chromosome 3p26 and is a candidate gene for 3p- syndrome characterized by mental impairment (7). A missense CHL1 polymorphism associated increased risk of schizophrenia, has also been reported (8). The functional importance of CHL-1 in the nervous system is also evident in CHL-1 deficient mice, which display behavioral abnormalities and show misguided axons within the hippocampus and olfactory tract (9). Enhanced ectodomain-shedding of CHL-1 is also observed in Wobbler mice, the neurodegenerative mutant mice (6). In vitro, soluble or substrate-coated CHL-1 promotes neurite outgrowth and neuronal survival of both cerebellar and hippocampal neurons. Cell surface CHL-1 interacts with integrins in cis to potentiate integrin-dependent cell migration toward extracellular matrix proteins (10). For this enhanced cell motility, CHL-1 linkage to the actin cytoskeleton via interaction between ankyrin and the CHL-1 cytoplasmic region is required.

  • References:
    1. Moos, M. et al. (1988) Nature 334:701.
    2. Holm, J. et al. (1996) Eur. J. Neusci. 8:1613.
    3. Wei, M. et al. (1998) Hum. Genet. 103:355.
    4. Hillenbrand, R. et al. (1999) Eur. J. Neurosci. 11:813.
    5. Liu, Q. et al. (2000) J. Neurosci. 20:7682.
    6. Naus, S. et al. (2004) J. Biol. Chem. 279:16083.
    7. Angeloni, D. et al. (1999) Am. J. Med. Genet. 86:482.
    8. Sakurai, K. et al. (2002) Mol. Psychiatry 7:412.
    9. Montag-Sallaz, M. et al. (2002) Mol. Cell. Biol. 22:7967.
    10. Buhusi, M. et al. (2003) J. Biol. Chem. 278(27):25024.
  • Long Name:
    Cell Adhesion Molecule with Homology to L1CAM
  • Entrez Gene IDs:
    10752 (Human); 12661 (Mouse)
  • Alternate Names:
    CALL; CALLClose homolog of L1; cell adhesion molecule with homology to L1CAM (close homolog of L1); cell adhesion molecule with homology to L1CAM (close homologue of L1); CHL1; CHL-1; FLJ44930; L1CAM-2; L1CAM2L1 cell adhesion molecule 2; MGC132578; neural cell adhesion molecule L1-like protein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Applications
Sample Type
  1. Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors.
    Authors: Bye C, Jonsson M, Bjorklund A, Parish C, Thompson L
    Proc Natl Acad Sci U S A, 2015;112(15):E1946-55.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC- Not specified
  2. An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's disease.
    Authors: Kizuka Y, Kitazume S, Fujinawa R, Saito T, Iwata N, Saido T, Nakano M, Yamaguchi Y, Hashimoto Y, Staufenbiel M, Hatsuta H, Murayama S, Manya H, Endo T, Taniguchi N
    EMBO Mol Med, 2015;7(2):175-89.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. beta-Site amyloid precursor protein (APP)-cleaving enzyme 1 (BACE1)-deficient mice exhibit a close homolog of L1 (CHL1) loss-of-function phenotype involving axon guidance defects.
    Authors: Hitt B, Riordan S, Kukreja L, Eimer W, Rajapaksha T, Vassar R
    J Biol Chem, 2012;287(46):38408-25.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. EphB regulates L1 phosphorylation during retinocollicular mapping.
    Mol. Cell. Neurosci., 2012;50(2):201-10.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
  5. Secretome protein enrichment identifies physiological BACE1 protease substrates in neurons.
    Authors: Kuhn PH, Koroniak K, Hogl S, Colombo A, Zeitschel U, Willem M, Volbracht C, Schepers U, Imhof A, Hoffmeister A, Haass C, Rossner S, Brase S, Lichtenthaler SF
    EMBO J., 2012;31(14):3157-68.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. CHL1 negatively regulates the proliferation and neuronal differentiation of neural progenitor cells through activation of the ERK1/2 MAPK pathway.
    Authors: Huang X, Zhu LL, Zhao T
    Mol. Cell. Neurosci., 2011;46(1):296-307.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Phosphatidylinositol 3-kinase/protein kinase Cdelta activation induces close homolog of adhesion molecule L1 (CHL1) expression in cultured astrocytes.
    Authors: Wu J, Wrathall JR, Schachner M
    Glia, 2010;58(3):315-28.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  8. CHL1 promotes Sema3A-induced growth cone collapse and neurite elaboration through a motif required for recruitment of ERM proteins to the plasma membrane.
    Authors: Schlatter MC, Buhusi M, Wright AG, Maness PF
    J. Neurochem., 2007;104(3):731-44.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
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Images Ratings Applications Species Reviewed By Date Details
Western Blot Mouse CHL‑1/L1CAM‑2 Antibody AF2147
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 WB Mouse Anonymous 04/26/2016
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Western Blot Mouse CHL‑1/L1CAM‑2 Antibody AF2147
Western Blot: Mouse CHL‑1/L1CAM‑2 Antibody [AF2147]

Summary

ApplicationWestern Blot
Sample TestedBrain tissue
SpeciesMouse

Other Experimental Details

Other Experimental Details80, 40 and 20ug of total mouse brain homogenate was separated by SDS-PAGE and transfered to PVDF membraneBlock: 1% BSA, 1% FSG PBS-T 1h RTPrimary: 1:1000, 1% BSA, 1% FSG PBS-T O/N 4oCSecondary: 1:5000,1% BSA, 1% FSG PBS-T 2h RT(Secondary - DaG 800, Licor)

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