Detects mouse Common gamma Chain/ IL-2 R gamma in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant
human Common gamma Chain, recombinant mouse (rm) IL-2 R alpha,
or rmIL-2 R beta is
Monoclonal Rat IgG2A Clone # 644337
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf21-derived recombinant mouse Common gamma Chain/ IL-2 R gamma Ser25-Ala263 (predicted) Accession # P34902
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse Common gamma Chain/IL‑2 R gamma by Western Blot. Western blot shows lysates of mouse lymphocytes. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Common gamma Chain/IL‑2 R gamma Monoclonal Antibody (Catalog # MAB7843) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Common gamma Chain/IL‑2 R gamma at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Common gamma Chain/IL-2 R gamma
The Common gamma Chain, also known as CD132, is a transmembrane protein belonging to the hematopoietin receptor family. gamma c is a signaling component of the functional receptor complexes for IL-2, IL-4, IL-7, IL-9, and IL-15.
IL-2 Receptor gamma
Entrez Gene IDs:
3561 (Human); 16186 (Mouse)
CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; P64; SCIDX; SCIDX1; severe combined immunodeficiency
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.