Mouse Common  gamma  Chain/IL-2 R gamma Antibody

Catalog # Availability Size / Price Qty
MAB7843
MAB7843-SP
Detection of Mouse Common  gamma  Chain/IL‑2 R gamma  by Western Blot.
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Product Details
Citations (1)
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Mouse Common  gamma  Chain/IL-2 R gamma Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Common gamma Chain/ IL-2 R gamma in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Common gamma Chain, recombinant mouse (rm) IL-2 R alpha, or rmIL-2 R beta is observed.
Source
Monoclonal Rat IgG2A Clone # 644337
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf21-derived recombinant mouse Common gamma Chain/ IL-2 R gamma
Ser25-Ala263 (predicted)
Accession # P34902
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse Common  gamma  Chain/IL‑2 R gamma  by Western Blot. View Larger

Detection of Mouse Common  gamma  Chain/IL‑2 R gamma by Western Blot. Western blot shows lysates of mouse lymphocytes. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Common  gamma  Chain/IL‑2 R gamma Monoclonal Antibody (Catalog # MAB7843) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Common  gamma  Chain/IL‑2 R gamma at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Common gamma Chain/IL-2 R gamma

The Common gamma Chain, also known as CD132, is a transmembrane protein belonging to the hematopoietin receptor family. gamma c is a signaling component of the functional receptor complexes for IL-2, IL-4, IL-7, IL-9, and IL-15.

Long Name
IL-2 Receptor gamma
Entrez Gene IDs
3561 (Human); 16186 (Mouse)
Alternate Names
CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; Common gamma Chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; P64; SCIDX; SCIDX1; severe combined immunodeficiency

Product Datasheets

Citation for Mouse Common  gamma  Chain/IL-2 R gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Interleukin 15 activates Akt to protect astrocytes from oxygen glucose deprivation-induced cell death
    Authors: GA Lee, YG Lai, RJ Chen, NS Liao
    Cytokine, 2017;92(0):68-74.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB

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