Mouse CXCL4/PF4 Antibody

Catalog #: AF595
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF595
AF595-SP

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Detection of Mouse CXCL4/PF4 by Western Blot.

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Citations (1)

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Mouse CXCL4/PF4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CXCL4/PF4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human CXCL4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse CXCL4/PF4
Val30-Ser105
Accession # Q9Z126

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

25 µg/mL

Mouse platelets

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse CXCL4/PF4 by Western Blot. Western blot shows lysate of mouse platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF595) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Mouse CXCL4/PF4 by Simple Western^TM. Simple Western lane view shows lysates of mouse platelets, loaded at 0.2 mg/mL. A specific band was detected for CXCL4/PF4 at approximately 14 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF595). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL4/PF4

CXCL4 is a member of the CXC chemokine family. Mouse CXCL4 is a 105 amino acid (aa) protein with a 29 aa signal sequence and a 76 aa mature protein. CXCL4 has homology with IL-8 and beta -thromboglobulin. Mouse and human CXCL4 share a 64% identity. Mouse and rat CXCL4 share 89% identity. CXCL4 contains several heparin-binding sites at the C-terminal region. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other fuctions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, and it has anti-angiogenic activity. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.

References

Poncz, M. et al. (1987) Blood 69:219.
Scheuerer, B. et al. (2000) Blood 95:1158.
Perollet, C. et al. (1998) Blood 91:3289.
Petersen, F. et al. (1998) J. Immunol. 161:4347.
Petersen, F. et al. (1999) J. Biol. Chem. 274:12376.
Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.

Entrez Gene IDs

5196 (Human); 56744 (Mouse); 360918 (Rat)

Alternate Names

chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; CXCL4; CXCL4iroplact; Iroplact; MGC138298; Oncostatin-A; PF4; PF-4; platelet factor 4; SCYB4oncostatin-A

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Mouse CXCL4/PF4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Comprehensive assessment of chemokine expression profiles by flow cytometry.
Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
J. Clin. Invest., 2010;120(3):907-23.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry