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Mouse CXCL7/Thymus Chemokine-1 Antibody

Catalog #: AF793
3 Citations Datasheet / COA / SDS
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AF793
AF793-SP

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CXCL7/Thymus Chemokine‑1 in Mouse Thymus.
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Citations (3)
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Mouse CXCL7/Thymus Chemokine-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects CXCL7/Thymus Chemokine‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant rat CXCL7/Thymus Chemokine-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CXCL7/Thymus Chemokine-1
Lys40-Tyr113
Accession # AAG36786
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CXCL7/Thymus Chemokine‑1 (Catalog # 793-CK)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize CXCL7/Thymus Chemokine‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.8-4 µg/mL in the presence of 40 ng/mL Recombinant Mouse CXCL7/Thymus Chemokine‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry CXCL7/Thymus Chemokine‑1 antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr). View Larger

CXCL7/Thymus Chemokine‑1 in Mouse Thymus. CXCL7/Thymus Chemokine-1 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CXCL7/Thymus Chemokine-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF793) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Neutralization Chemotaxis Induced by CXCL7/Thymus Chemo-kine‑1 and Neutralization by Mouse CXCL7/Thymus Chemo-kine‑1 Antibody. View Larger

Chemotaxis Induced by CXCL7/Thymus Chemo-kine‑1 and Neutralization by Mouse CXCL7/Thymus Chemo-kine‑1 Antibody. Recombinant Mouse CXCL7/Thymus Chemo-kine-1 (Catalog # 1091-CK) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL7/Thymus Chemo-kine-1 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL7/Thymus Chemo-kine-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF793). The ND50 is typically 0.8-4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL7/Thymus Chemokine-1

CXCL7 is a member of the alpha (CXC) subfamily of chemokines. Mouse CXCL7 cDNA encodes a 113 amino acid residue precursor protein. Signal P prediction program analysis indicates that this precursor protein contains an amino terminal 39 amino acid residue signal peptide and a 74 amino acid mature chemokine. Mouse CXCL7 shares 72% amino acid sequence identity with rat CXCL7. The recombinant mouse CXCL7 produced by R&D Systems has been shown to chemoattract mouse BaF3 cells transfected with human CXCR2, but not human CXCR1.

Entrez Gene IDs
57349 (Mouse); 246358 (Rat)
Alternate Names
Beta-TG;B-TG1;CTAP3;CTAPIII;CTAP-III;CXCL7;LA-PF4;LDGF;MDGF;NAP-2;PBP;Platelet basic protein;PPBP;SCYB7;TC1;TC2;TGB;TGB1;THBGB;THBGB1; Thymus Chemokine1; Thymus Chemokine-1

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Citations for Mouse CXCL7/Thymus Chemokine-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Host-Derived Matrix Metalloproteinase-13 Activity Promotes Multiple Myeloma–Induced Osteolysis and Reduces Overall Survival
    Authors: Chen Hao Lo, Gemma Shay, Jeremy J. McGuire, Tao Li, Kenneth H. Shain, Jun Yong Choi et al.
    Cancer Research
  2. CXCR4+CD45- cells are niche forming for osteoclastogenesis via the SDF-1, CXCL7, and CX3CL1 signaling pathways in bone marrow
    Authors: Yoh Goto
    Stem Cells, 2016-07-08;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy.
    Authors: Wang H, Hong L, Huang J, Jiang Q, Tao R, Tan C, Lu N, Wang C, Ahmed M, Lu Y, Liu Z, Shi W, Lai E, Wilcox C, Han F
    Cell Res, 2015-05-22;25(6):674-90.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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