Mouse Endomucin Antibody

Catalog # Availability Size / Price Qty
Detection of Mouse Endomucin by Western Blot.
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Product Details
Citations (12)
Supplemental Products
Reviews (1)

Mouse Endomucin Antibody Summary

Species Reactivity
Detects mouse Endomucin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Endomucin-2 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant mouse Endomucin
Accession # Q9R0H2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Endomucin antibody by Western Blot. View Larger

Detection of Mouse Endomucin by Western Blot. Western blot shows lysates of mouse kidney tissue, mouse lung tissue, and mouse ovary tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Endomucin at approximately 75-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Endomucin antibody in bEnd by Immunocytochemistry (ICC).3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Endomucin in bEnd.3 Mouse Cell Line. Endomucin was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow Cytometry Detection of Endomucin antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Endomucin in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endomucin

Endomucin (endothelial sialomucin; also Endomucin-1/2 and Mucin-14) is an 80‑120 kDa glycoprotein member of the Endomucin family of proteins. It is expressed on endothelial cells and depending upon its glycosylation pattern, can serve as either a pro- or anti-adhesive molecule. Mouse Endomucin precursor is 261 amino acids in length. It is type I transmembrane protein that contains a 170 aa extracellular domain (ECD) (aa 21‑190) and a 50 aa cytoplasmic region. Three splice variants exist in the ECD. One shows a deletion of aa 91‑141, a second shows a one aa substitution for aa 91‑129, and a third shows a one aa substitution for aa 129‑142. Over
aa 21‑90, mouse Endomucin shares 60% and 30% aa identity with rat and human Endomucin, respectively.

Entrez Gene IDs
51705 (Human); 59308 (Mouse); 295490 (Rat)
Alternate Names
EMCN; EMCN2; Endomucin; MUC-14

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Citations for Mouse Endomucin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Ventromedial hypothalamic OGT drives adipose tissue lipolysis and curbs obesity
    Authors: Q Wang, B Zhang, B Stutz, ZW Liu, TL Horvath, X Yang
    Science Advances, 2022;8(35):eabn8092.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Endothelial TrkA coordinates vascularization and innervation in thermogenic adipose tissue and can be targeted to control metabolism
    Authors: AC Daquinag, Z Gao, Y Yu, MG Kolonin
    Molecular Metabolism, 2022;63(0):101544.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair
    Authors: EC Jeffery, TLA Mann, JA Pool, Z Zhao, SJ Morrison
    Cell Stem Cell, 2022;29(11):1547-1561.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Bone marrow CD73+ mesenchymal stem cells display increased stemness in vitro and promote fracture healing in vivo
    Authors: K Kimura, M Breitbach, FA Schildberg, M Hesse, BK Fleischman
    Bone Reports, 2021;15(0):101133.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Impairment of type H vessels by NOX2-mediated endothelial oxidative stress: critical mechanisms and therapeutic targets for bone fragility in streptozotocin-induced type 1 diabetic mice
    Authors: XF Hu, G Xiang, TJ Wang, YB Ma, Y Zhang, YB Yan, X Zhao, ZX Wu, YF Feng, W Lei
    Theranostics, 2021;11(8):3796-3812.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. VCAM-1 Density and Tumor Perfusion Predict T-cell Infiltration and Treatment Response in Preclinical Models
    Authors: J Riegler, H Gill, A Ogasawara, M Hedehus, V Javinal, J Oeh, GZ Ferl, J Marik, S Williams, D Sampath, J Schartner, RAD Carano
    Neoplasia, 2019;21(10):1036-1050.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Wnt/?-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction
    Authors: J Miao, J Liu, J Niu, Y Zhang, W Shen, C Luo, Y Liu, C Li, H Li, P Yang, Y Liu, FF Hou, L Zhou
    Aging Cell, 2019;0(0):e13004.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Inverted formin 2 regulates intracellular trafficking, placentation, and pregnancy outcome
    Authors: KYB Lamm, ML Johnson, J Baker Phil, MB Muntiferin, JM James, HN Jones, RW Redline, A Rokas, LJ Muglia
    Elife, 2018;7(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. PECAM/eGFP transgenic mice for monitoring of angiogenesis in health and disease
    Authors: F Winkler, K Herz, S Rieck, K Kimura, T Hu, W Röll, M Hesse, BK Fleischman, D Wenzel
    Sci Rep, 2018;8(1):17582.
  10. Endothelial Cells Are Not Required for Specification of Respiratory Progenitors
    Authors: JA Havrilak, KR Melton, JM Shannon
    Dev. Biol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Suppression of ischemia in arterial occlusive disease by JNK-promoted native collateral artery development
    Elife, 2016;5(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. Sox17 is required for normal pulmonary vascular morphogenesis.
    Authors: Lange A, Haitchi H, LeCras T, Sridharan A, Xu Y, Wert S, James J, Udell N, Thurner P, Whitsett J
    Dev Biol, 2014;387(1):109-20.


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Mouse Endomucin Antibody
By Anonymous on 08/31/2018
Application: ICC/IF Sample Tested: bone marrow Species: Mouse