Mouse Endomucin Antibody

Catalog # Availability Size / Price Qty
AF4666
AF4666-SP

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Detection of Mouse Endomucin by Western Blot.
8 Images
Product Details
Citations (14)
FAQs
Supplemental Products
Reviews (1)

Mouse Endomucin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Endomucin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Endomucin-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Endomucin
Glu21-Glu90
Accession # Q9R0H2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Endomucin antibody by Western Blot. View Larger

Detection of Mouse Endomucin by Western Blot. Western blot shows lysates of mouse kidney tissue, mouse lung tissue, and mouse ovary tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Endomucin at approximately 75-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Endomucin antibody in bEnd by Immunocytochemistry (ICC).3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Endomucin in bEnd.3 Mouse Cell Line. Endomucin was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow Cytometry Detection of Endomucin antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Endomucin in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Immunocytochemistry/ Immunofluorescence Detection of Mouse Endomucin/Mucin -14 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Endomucin/Mucin -14 by Immunocytochemistry/Immunofluorescence MLK2/3-deficient mice exhibit defects in native collateral artery formation.(A) Representative confocal images (n = 5 mice) of control and MLK2/3-deficient whole mount P6 adductor muscle vasculature stained with antibodies to endomucin (capillary and venous vasculature, red) and SMA (arterial and venous smooth muscle, green). Gracilis collateral arteries in WT mice, but not MLK2/3-deficient mice, interconnect the PCFA to the SA. (B) Representative stereomicroscope images of P6 whole mount abdominal muscle stained with an antibody to SMA (green). Arteriole-to-arteriole arcades are indicated (red arrows). The abdominal muscle vasculature of Map3k10-/-Map3k11-/- mice shows very few arteriole-to-arteriole interconnections. Quantitation reveals significantly reduced arteriolar arcade numbers in Map3k10-/-Map3k11-/- mice compared to WT mice (mean ± SEM; n = 5 mice). Source data are included as Figure 3—source data 1.DOI:http://dx.doi.org/10.7554/eLife.18414.02310.7554/eLife.18414.024Figure 3—source data 1.Source data for Figure 3.This file contains raw source data used to make the graphs presented in Figure 3.DOI:http://dx.doi.org/10.7554/eLife.18414.024Source data for Figure 3.This file contains raw source data used to make the graphs presented in Figure 3.DOI:http://dx.doi.org/10.7554/eLife.18414.024 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27504807), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Endomucin/Mucin -14 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Endomucin/Mucin -14 by Immunocytochemistry/Immunofluorescence NOX2 contributes to the oxidative stress of bone ECs and the damage of angiogenesis-osteogenesis coupling. (A) qPCR analysis of the relative mRNA levels of three critical NOXs, involved in endothelial oxidative injuries, in the ECs in tibia metaphysis. T1DM mice were intraperitoneally injected with GKT137831 (NOX1/4 inhibitor) or one of the two NOX2 inhibitors, NOX2ds-tat (NOX2ds) and GSK2795039 (GSK), or VAS2870 (VAS, a pan-NOX inhibitor) once every two days from week 4 through 8. The levels of blood glucose (B), insulin (C) and body weight (D) during treatments were tested. (E) Confocal images of immunostained tibia sections show the CD31+Emcn+ type H vessels in the metaphysis at week 8. Scale bar: 50 μm on the above and 20 μm on the below. (F and G) Histomorphometric quantitation of the vessels in proximal tibia metaphysis. Flow cytometry quantification of type H ECs and total ECs in 8-week-old tibia (H). (I) Flow cytometry analysis of intracellular DCF fluorescence shows the ROS levels in the ECs in 8-week-old tibia. (B)-(D), n = 15-16 per group; other panels, n = 7-8 per group. ###p < 0.001 vs. Control group; *p < 0.05, **p < 0.01 and ***p < 0.001 vs. DM+veh group. One-way or two-way ANOVA followed by Tukey posttest analysis. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33664862), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Endomucin/Mucin -14 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Endomucin/Mucin -14 by Immunocytochemistry/Immunofluorescence The effects of anti-oxidant treatments on the bone blood vessels of T1DM mice. T1D mice received intraperitoneal injection of an anti-oxidant, N-acetyl cysteine (NAC) or tempol (TPO), once every other day from week 4 through 8. (A) Flow cytometry analysis of the general ROS levels in the bone ECs from 8-week-old tibia by detecting intracellular DCF fluorescence. The levels of blood glucose (B), insulin (C) and body weight (D). (E) Tibia length at week 8. (F) Confocal images of CD31 (green) and Emcn (red) immunostained tibia sections show type H vessels in the tibia metaphysis at week 8. Scale bar: 50 μm on the above and 20 μm on the below. Histomorphometric quantitation of the area fraction of vessel columns (G) and the number of vascular buds per growth plate length (H). Flow cytometry quantification of type H ECs (I) and total ECs (J) in 8-week-old tibia. (K) qPCR analysis of the representative cytokines mediating the coupling of angiogenesis and osteogenesis in 8-week-old tibia. (B)-(D), n = 15-16 per group; other panels, n = 7-8 per group. *p < 0.05, **p < 0.01 and ***p < 0.001. One-way or two-way ANOVA followed by Tukey posttest analysis. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33664862), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Endomucin/Mucin -14 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Endomucin/Mucin -14 by Immunocytochemistry/Immunofluorescence Endothelial JNK-deficient mice display abnormal native collateral arteries.(A,B) Representative confocal images (n = 7 mice) of whole mount adductor muscle vasculature reveals SMA-covered gracilis collateral arteries in P6 control mice, but not JNK-deficient mice (A). Confocal imaging of Dil perfused P6 adductor muscle vasculature (n = 5 mice) demonstrates distinct gracilis collaterals interconnecting the PCFA to the SA in control mice. Vessels emerging from the PCFA and the SA in E3KO mice do not fully interconnect, but branch into smaller vessels. At P0, gracilis collaterals were not SMA-covered, but were perfused with Dil in control mice (B). The analogous vessels in E3KO mice did not interconnect, but branched extensively into smaller vessels (B) (n = 5 mice). (C) Representative stereomicroscope images of Dil-perfused abdominal muscle arterial vasculature of control and JNK-deficient P0 mice. Arteriole-to-arteriole arcades (indicated by red arrows) were quantitated (mean ± SEM; n = 3 mice). (D) Representative confocal images (n = 3~4 mice) of whole mount adductor muscles of control and JNK-deficient E16.5 embryos showing GFP-labeled vascular endothelial cells. (E) Representative confocal images (n = 3~5 mice) of control and JNK-deficient E16.5 embryo adductor muscle vasculature immunostained for Endomucin (Emcn, red) and isolectinB4 (iB4, green). Prominent vessels emerging from the PCFA and SA are indicated with white arrowheads. Source data are included as Figure 5—source data 1.DOI:http://dx.doi.org/10.7554/eLife.18414.02710.7554/eLife.18414.028Figure 5—source data 1.Source data for Figure 5.This file contains raw source data used to make the graphs presented in Figure 5.DOI:http://dx.doi.org/10.7554/eLife.18414.028Source data for Figure 5.This file contains raw source data used to make the graphs presented in Figure 5.DOI:http://dx.doi.org/10.7554/eLife.18414.028Intimate association of gracilis collaterals and peripheral nerves in adductor muscles.Confocal microscopy of a whole mount adductor muscle stained with antibodies to SMA (green) and Neurofilament-M (red) illustrates the close association of gracilis collateral arteries with peripheral nerves.DOI:http://dx.doi.org/10.7554/eLife.18414.029 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27504807), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Endomucin/Mucin -14 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Endomucin/Mucin -14 by Immunocytochemistry/Immunofluorescence The effects of insulin therapy on the bone blood vessels of T1DM mice. Drug therapy with high dose (hi, 0.5 U/mouse) or low dose (lo, 0.05 U/mouse) of insulin (ins) or the combination of insulin and a relatively low dose of GSK2795039 (GSK, a NOX2 inhibitor, 2 mM) was conducted in two time courses: started immediately after the confirmation of DM and once per day in week 4 to 8 (4-8w); started at week 6 and performed once per day in week 6 to 8 (6-8w). The levels of blood glucose (A) and body weight (B) were examined at week 4, 6 and 8, with HbA1c (C) tested at week 8. (D) Confocal images of immunostained tibia sections show CD31+ (green) and Emcn+ (red) type H vessels in the metaphysis. Scale bar: 50 μm on the above and 20 μm on the below. Histomorphometric quantitation as the area fraction of vessel columns (E) and the number of vascular buds per growth plate length (F). (G) Flow cytometry quantification of type H ECs and total ECs in 8-week-old tibia. For drug treatments, T1DM model was induced by STZ injection at 80 mg/kg (namely DM-G2). (A)-(C), n = 15-16 per group; (E)-(G), n = 6-8 per group. *p < 0.05, **p < 0.01 and ***p < 0.001 vs. DM+veh group; #p < 0.05, ##p < 0.01 and ###p < 0.001 vs. DM+ins hi 6-8w group; &p < 0.05, &&p < 0.01 and &&&p < 0.001 vs. DM+ins lo 4-8w group. One-way or two-way ANOVA followed by Tukey posttest analysis. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33664862), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endomucin

Endomucin (endothelial sialomucin; also Endomucin-1/2 and Mucin-14) is an 80‑120 kDa glycoprotein member of the Endomucin family of proteins. It is expressed on endothelial cells and depending upon its glycosylation pattern, can serve as either a pro- or anti-adhesive molecule. Mouse Endomucin precursor is 261 amino acids in length. It is type I transmembrane protein that contains a 170 aa extracellular domain (ECD) (aa 21‑190) and a 50 aa cytoplasmic region. Three splice variants exist in the ECD. One shows a deletion of aa 91‑141, a second shows a one aa substitution for aa 91‑129, and a third shows a one aa substitution for aa 129‑142. Over
aa 21‑90, mouse Endomucin shares 60% and 30% aa identity with rat and human Endomucin, respectively.

Entrez Gene IDs
51705 (Human); 59308 (Mouse); 295490 (Rat)
Alternate Names
EMCN; EMCN2; Endomucin; MUC-14

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Citations for Mouse Endomucin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Leptin receptor+ cells promote bone marrow innervation and regeneration by synthesizing nerve growth factor
    Authors: Gao, X;Murphy, MM;Peyer, JG;Ni, Y;Yang, M;Zhang, Y;Guo, J;Kara, N;Embree, C;Tasdogan, A;Ubellacker, JM;Crane, GM;Fang, S;Zhao, Z;Shen, B;Morrison, SJ;
    Nature cell biology
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Atf3 defines a population of pulmonary endothelial cells essential for lung regeneration
    Authors: Niethamer, TK;Levin, LI;Morley, MP;Babu, A;Zhou, S;Morrisey, EE;
    eLife
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair
    Authors: EC Jeffery, TLA Mann, JA Pool, Z Zhao, SJ Morrison
    Cell Stem Cell, 2022-10-21;29(11):1547-1561.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Ventromedial hypothalamic OGT drives adipose tissue lipolysis and curbs obesity
    Authors: Q Wang, B Zhang, B Stutz, ZW Liu, TL Horvath, X Yang
    Science Advances, 2022-08-31;8(35):eabn8092.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Endothelial TrkA coordinates vascularization and innervation in thermogenic adipose tissue and can be targeted to control metabolism
    Authors: AC Daquinag, Z Gao, Y Yu, MG Kolonin
    Molecular Metabolism, 2022-07-11;63(0):101544.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Bone marrow CD73+ mesenchymal stem cells display increased stemness in vitro and promote fracture healing in vivo
    Authors: K Kimura, M Breitbach, FA Schildberg, M Hesse, BK Fleischman
    Bone Reports, 2021-09-29;15(0):101133.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Impairment of type H vessels by NOX2-mediated endothelial oxidative stress: critical mechanisms and therapeutic targets for bone fragility in streptozotocin-induced type 1 diabetic mice
    Authors: XF Hu, G Xiang, TJ Wang, YB Ma, Y Zhang, YB Yan, X Zhao, ZX Wu, YF Feng, W Lei
    Theranostics, 2021-01-30;11(8):3796-3812.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  8. VCAM-1 Density and Tumor Perfusion Predict T-cell Infiltration and Treatment Response in Preclinical Models
    Authors: J Riegler, H Gill, A Ogasawara, M Hedehus, V Javinal, J Oeh, GZ Ferl, J Marik, S Williams, D Sampath, J Schartner, RAD Carano
    Neoplasia, 2019-09-11;21(10):1036-1050.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  9. Wnt/?-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction
    Authors: J Miao, J Liu, J Niu, Y Zhang, W Shen, C Luo, Y Liu, C Li, H Li, P Yang, Y Liu, FF Hou, L Zhou
    Aging Cell, 2019-07-18;0(0):e13004.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  10. PECAM/eGFP transgenic mice for monitoring of angiogenesis in health and disease
    Authors: F Winkler, K Herz, S Rieck, K Kimura, T Hu, W Röll, M Hesse, BK Fleischman, D Wenzel
    Sci Rep, 2018-12-04;8(1):17582.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Inverted formin 2 regulates intracellular trafficking, placentation, and pregnancy outcome
    Authors: KYB Lamm, ML Johnson, J Baker Phil, MB Muntiferin, JM James, HN Jones, RW Redline, A Rokas, LJ Muglia
    Elife, 2018-01-08;7(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Endothelial Cells Are Not Required for Specification of Respiratory Progenitors
    Authors: JA Havrilak, KR Melton, JM Shannon
    Dev. Biol., 2017-05-10;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Suppression of ischemia in arterial occlusive disease by JNK-promoted native collateral artery development
    Elife, 2016-08-09;5(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  14. Sox17 is required for normal pulmonary vascular morphogenesis.
    Authors: Lange A, Haitchi H, LeCras T, Sridharan A, Xu Y, Wert S, James J, Udell N, Thurner P, Whitsett J
    Dev Biol, 2014-01-10;387(1):109-20.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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Mouse Endomucin Antibody
By Anonymous on 08/31/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: bone marrow Species: Mouse