|Detection of Mouse Endomucin by Western Blot. Western blot shows lysates of mouse kidney tissue, mouse lung tissue, and mouse ovary tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Endomucin at approximately 75-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|Detection of Endomucin in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).|
|Endomucin in bEnd.3 Mouse Cell Line. Endomucin was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
Endomucin (endothelial sialomucin; also Endomucin-1/2 and Mucin-14) is an 80‑120 kDa glycoprotein member of the Endomucin family of proteins. It is expressed on endothelial cells and depending upon its glycosylation pattern, can serve as either a pro- or anti-adhesive molecule. Mouse Endomucin precursor is 261 amino acids in length. It is type I transmembrane protein that contains a 170 aa extracellular domain (ECD) (aa 21‑190) and a 50 aa cytoplasmic region. Three splice variants exist in the ECD. One shows a deletion of aa 91‑141, a second shows a one aa substitution for aa 91‑129, and a third shows a one aa substitution for aa 129‑142. Over
aa 21‑90, mouse Endomucin shares 60% and 30% aa identity with rat and human Endomucin, respectively.
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