Mouse Endomucin Antibody
Mouse Endomucin Antibody Summary
Glu21-Glu90
Accession # Q9R0H2
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse Endomucin by Western Blot. Western blot shows lysates of mouse kidney tissue, mouse lung tissue, and mouse ovary tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Endomucin at approximately 75-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Endomucin in bEnd.3 Mouse Cell Line. Endomucin was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Endomucin in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endomucin
Endomucin (endothelial sialomucin; also Endomucin-1/2 and Mucin-14) is an 80‑120 kDa glycoprotein member of the Endomucin family of proteins. It is expressed on endothelial cells and depending upon its glycosylation pattern, can serve as either a pro- or anti-adhesive molecule. Mouse Endomucin precursor is 261 amino acids in length. It is type I transmembrane protein that contains a 170 aa extracellular domain (ECD) (aa 21‑190) and a 50 aa cytoplasmic region. Three splice variants exist in the ECD. One shows a deletion of aa 91‑141, a second shows a one aa substitution for aa 91‑129, and a third shows a one aa substitution for aa 129‑142. Over
aa 21‑90, mouse Endomucin shares 60% and 30% aa identity with rat and human Endomucin, respectively.
Product Datasheets
Citations for Mouse Endomucin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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Ventromedial hypothalamic OGT drives adipose tissue lipolysis and curbs obesity
Authors: Q Wang, B Zhang, B Stutz, ZW Liu, TL Horvath, X Yang
Science Advances, 2022;8(35):eabn8092.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Endothelial TrkA coordinates vascularization and innervation in thermogenic adipose tissue and can be targeted to control metabolism
Authors: AC Daquinag, Z Gao, Y Yu, MG Kolonin
Molecular Metabolism, 2022;63(0):101544.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair
Authors: EC Jeffery, TLA Mann, JA Pool, Z Zhao, SJ Morrison
Cell Stem Cell, 2022;29(11):1547-1561.e6.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Bone marrow CD73+ mesenchymal stem cells display increased stemness in vitro and promote fracture healing in vivo
Authors: K Kimura, M Breitbach, FA Schildberg, M Hesse, BK Fleischman
Bone Reports, 2021;15(0):101133.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Impairment of type H vessels by NOX2-mediated endothelial oxidative stress: critical mechanisms and therapeutic targets for bone fragility in streptozotocin-induced type 1 diabetic mice
Authors: XF Hu, G Xiang, TJ Wang, YB Ma, Y Zhang, YB Yan, X Zhao, ZX Wu, YF Feng, W Lei
Theranostics, 2021;11(8):3796-3812.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
VCAM-1 Density and Tumor Perfusion Predict T-cell Infiltration and Treatment Response in Preclinical Models
Authors: J Riegler, H Gill, A Ogasawara, M Hedehus, V Javinal, J Oeh, GZ Ferl, J Marik, S Williams, D Sampath, J Schartner, RAD Carano
Neoplasia, 2019;21(10):1036-1050.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Wnt/?-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction
Authors: J Miao, J Liu, J Niu, Y Zhang, W Shen, C Luo, Y Liu, C Li, H Li, P Yang, Y Liu, FF Hou, L Zhou
Aging Cell, 2019;0(0):e13004.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Inverted formin 2 regulates intracellular trafficking, placentation, and pregnancy outcome
Authors: KYB Lamm, ML Johnson, J Baker Phil, MB Muntiferin, JM James, HN Jones, RW Redline, A Rokas, LJ Muglia
Elife, 2018;7(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
PECAM/eGFP transgenic mice for monitoring of angiogenesis in health and disease
Authors: F Winkler, K Herz, S Rieck, K Kimura, T Hu, W Röll, M Hesse, BK Fleischman, D Wenzel
Sci Rep, 2018;8(1):17582.
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Endothelial Cells Are Not Required for Specification of Respiratory Progenitors
Authors: JA Havrilak, KR Melton, JM Shannon
Dev. Biol., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Suppression of ischemia in arterial occlusive disease by JNK-promoted native collateral artery development
Elife, 2016;5(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Sox17 is required for normal pulmonary vascular morphogenesis.
Authors: Lange A, Haitchi H, LeCras T, Sridharan A, Xu Y, Wert S, James J, Udell N, Thurner P, Whitsett J
Dev Biol, 2014;387(1):109-20.
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