Mouse Fas/TNFRSF6/CD95 Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Fas/TNFRSF6/CD95 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross‑reactivity with recombinant human (rh) Fas, rh4-1BB, rhDR6, rhNGF R, recombinant mouse (rm) OPG, rmRANK and rmCD30 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Fas/TNFRSF6/CD95
    Gly14-Arg169
    Accession # P25446
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Fas/TNFRSF6/CD95 Fc Chimera (Catalog # 435-FA)
  • Flow Cytometry
    2.5 µg/106 cells
    Mouse splenocytes
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
    • Mouse Fas/TNFRSF6/CD95 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse Fas/TNFRSF6/CD95 Antibody (Catalog # AF435)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse Fas/TNFRSF6/CD95 Biotinylated Antibody (Catalog # BAF435)
  • ELISA Standard
     
    Recombinant Mouse Fas/TNFRSF6/CD95 Fc Chimera Protein (Catalog # 435-FA)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Fas/TNFRSF6/CD95 in Mouse Embryo. Fas/TNFRSF6/CD95 was detected in immersion fixed frozen sections of mouse embryo (E15) using Goat Anti-Mouse Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF435) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to olfactory epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fas/TNFRSF6/CD95

Fas, also known as APO-1, CD95, and TNFRSF6, was originally identified as a cell-surface protein which binds to monoclonal antibodies that were cytolytic for various human cell lines. In the TNF receptor superfamily nomenclature, Fas is referred to as TNFRSF6. Human and mouse Fas cDNAs encode a 325 and a 327 amino acid residue type 1 membrane protein, respectively, that belongs to the TNF and NGF receptor family. Alternatively spliced cDNAs encoding multiple human Fas isoforms, including a soluble form of Fas lacking the transmembrane domain, have also been identified. Fas is highly expressed in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and other tumor cells. Fas expression has also been detected in mouse thymus, liver, heart, lung, kidney and ovary. The ligand for Fas (FasL) has been identified and shown to be a member of the TNF family of type 2 membrane proteins. FasL is predominantly expressed by activated T-lymphocytes, NK cells, and in tissues with immune-privileged sites. Soluble FasL can be produced by proteolysis of membrane-associated Fas.

Ligation of Fas by FasL or anti-Fas antibody has been shown to induce apoptotic cell death in Fas-bearing cells. Fas plays a role in the down-regulation of the immune reaction and has been shown to be a key mediator of activation-induced death of activated T lymphocytes. Fas-mediated cell death has also been shown to be important for the deletion of activated or autoreactive B lymphocytes. Besides the perforin/granzyme-based mechanism, the Fas system has been identified as the alternate pathway for CTL-mediated cytotoxicity. FasL has also been shown to function in immunological privileged sites by killing infiltrating Fas-bearing lymphocytes and inflammatory cells.

  • References:
    1. Nagata, S. and P. Golstein (1995) Science 267:1449.
    2. Nagata, S. (1997) Cell 88:355.
    3. Parijs, L. and A.K. Abbas (1996) Current Opinion in Immunol. 8:355.
    4. Green, D.R. and C.F. Ware (1997) Proc. Natl. Acad. Sci. USA. 94:5986.
  • Long Name:
    Fibroblast-associated
  • Entrez Gene IDs:
    355 (Human); 14102 (Mouse); 246097 (Rat); 493881 (Feline)
  • Alternate Names:
    Apo-1 antigen; Apo-1; apoptosis antigen 1; Apoptosis-mediating surface antigen FAS; APT1; APT1FASTM; CD95 antigen; CD95; CD95ALPS1A; Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; Fas; FAS1; FASLG receptor; TNFRSF6; TNFRSF6member 6; tumor necrosis factor receptor superfamily member 6
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Induced autoimmunity to heat shock proteins elicits glaucomatous loss of retinal ganglion cell neurons via activated T-cell-derived fas-ligand.
    Authors: Wax MB, Tezel G, Yang J, Peng G, Patil RV, Agarwal N, Sappington RM, Calkins DJ
    J. Neurosci., 2008;28(46):12085-96.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  2. Interleukin-17 as a recruitment and survival factor for airway macrophages in allergic airway inflammation.
    Authors: Sergejeva S, Ivanov S, Lotvall J, Linden A
    Am. J. Respir. Cell Mol. Biol., 2005;33(3):248-53.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
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Antigen Retrieval Reagent-Universal

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CTS014
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NL996
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VCTS021
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VCTS023
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VCTS022
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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