Mouse Fas/TNFRSF6/CD95 Antibody Summary
Accession # P25446
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Mouse Fas/TNFRSF6/CD95 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Fas/TNFRSF6/CD95 in Mouse Embryo. Fas/TNFRSF6/CD95 was detected in immersion fixed frozen sections of mouse embryo (E15) using Goat Anti-Mouse Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF435) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to olfactory epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Fas, also known as APO-1, CD95, and TNFRSF6, was originally identified as a cell-surface protein which binds to monoclonal antibodies that were cytolytic for various human cell lines. In the TNF receptor superfamily nomenclature, Fas is referred to as TNFRSF6. Human and mouse Fas cDNAs encode a 325 and a 327 amino acid residue type 1 membrane protein, respectively, that belongs to the TNF and NGF receptor family. Alternatively spliced cDNAs encoding multiple human Fas isoforms, including a soluble form of Fas lacking the transmembrane domain, have also been identified. Fas is highly expressed in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and other tumor cells. Fas expression has also been detected in mouse thymus, liver, heart, lung, kidney and ovary. The ligand for Fas (FasL) has been identified and shown to be a member of the TNF family of type 2 membrane proteins. FasL is predominantly expressed by activated T-lymphocytes, NK cells, and in tissues with immune-privileged sites. Soluble FasL can be produced by proteolysis of membrane-associated Fas.
Ligation of Fas by FasL or anti-Fas antibody has been shown to induce apoptotic cell death in Fas-bearing cells. Fas plays a role in the down-regulation of the immune reaction and has been shown to be a key mediator of activation-induced death of activated T lymphocytes. Fas-mediated cell death has also been shown to be important for the deletion of activated or autoreactive B lymphocytes. Besides the perforin/granzyme-based mechanism, the Fas system has been identified as the alternate pathway for CTL-mediated cytotoxicity. FasL has also been shown to function in immunological privileged sites by killing infiltrating Fas-bearing lymphocytes and inflammatory cells.
- Nagata, S. and P. Golstein (1995) Science 267:1449.
- Nagata, S. (1997) Cell 88:355.
- Parijs, L. and A.K. Abbas (1996) Current Opinion in Immunol. 8:355.
- Green, D.R. and C.F. Ware (1997) Proc. Natl. Acad. Sci. USA. 94:5986.
Citations for Mouse Fas/TNFRSF6/CD95 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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CD8+ tissue-resident memory T cells promote liver fibrosis resolution by inducing apoptosis of hepatic stellate cells
Authors: Y Koda, T Teratani, PS Chu, Y Hagihara, Y Mikami, Y Harada, H Tsujikawa, K Miyamoto, T Suzuki, N Taniki, T Sujino, M Sakamoto, T Kanai, N Nakamoto
Nature Communications, 2021;12(1):4474.
Sample Types: Whole Tissue
Induced autoimmunity to heat shock proteins elicits glaucomatous loss of retinal ganglion cell neurons via activated T-cell-derived fas-ligand.
Authors: Wax MB, Tezel G, Yang J, Peng G, Patil RV, Agarwal N, Sappington RM, Calkins DJ
J. Neurosci., 2008;28(46):12085-96.
Sample Types: Whole Tissue
Interleukin-17 as a recruitment and survival factor for airway macrophages in allergic airway inflammation.
Authors: Sergejeva S, Ivanov S, Lotvall J, Linden A
Am. J. Respir. Cell Mol. Biol., 2005;33(3):248-53.
Sample Types: Whole Cells
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