Mouse Follistatin-related Gene Protein/
FLRG Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Follistatin-related Gene Protein/FLRG in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human FLRG is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Follistatin-related Gene Protein/FLRG
    Val24-Val256
    Accession # Q9EQC7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Follistatin-related Gene Protein/FLRG (Catalog # 1255-F3)
  • Immunohistochemistry
    5-15 µg/mL
    Perfusion fixed frozen sections of mouse skin
  • Neutralization
    Measured by its ability to neutralize Follistatin‑related Gene Protein/FLRG inhibition of Activin A-dependent hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 0.1 µg/mL Recombinant Mouse Follistatin‑related Gene Protein/FLRG and 7.5 ng/mL Recombinant Human/Mouse/Rat Activin A.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Follistatin-related Gene Protein/FLRG Inhibition of Activin A-induced Hemoglobin Expression and Neutralization by Mouse Follistatin-related Gene Protein/FLRG Antibody. Recombinant Mouse Follistatin-related Gene Protein/FLRG (Catalog # 1255-F3) inhibits Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC) induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Inhibition of Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) activity elicited by Recombinant Mouse Follistatin-related Gene Protein/FLRG (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Follistatin-related Gene Protein/FLRG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1255). The ND50 is typically 3‑12 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Follistatin-related Gene Protein/FLRG

Follistatin-Related Gene Protein (FLRG), also known as follistatin-like 3 (FSTL3) is a glycoprotein belonging to the follistatin-module protein family. Mouse FLRG cDNA encodes a 256 amino acid (aa) residue protein with a putative 23 aa signal peptide, an N-terminal domain, two cysteine-rich follistatin-like domains (FS) and a C‑terminal acidic domain. Compared to follistatin, FLRG lacks the third FS domain found in follistatin. In addition, FLRG also lacks the heparin-binding domain found within the first amino-terminal FS domain of follistatin. Mouse and human FLRG share approximately 83% aa sequence homology. Like follistatin, FLRG has been shown to bind and inhibit the activities of TGF-beta family ligands including activin, BMP-2, -6, -7 and GDF-8/myostatin. While both FLRG and follistatin are located in a wide and overlapping range of adult and fetal tissue, their sites of peak expression differ: FLRG most highly in heart, lung, kidney, placenta and testis, while follistatin is highest in ovary and pituitary. The expression of FLRG is upregulated by TGF-beta and activin signaling through Smad proteins. Although FLRG is a secreted protein in many cell types, it has also been localized to the nuclear compartment in HeLa, 293 and CHO cells (1‑5).

  • References:
    1. Tsuchida, K. et al. (2000) J. Biol. Chem. 275:40778.
    2. Sidis, Y. et al. (2002) Endocrinology 143:1613.
    3. Tortoriello, D.V. et al. (2001) Endocrinology 142:3426.
    4. Hill, J. et al. (2002) J. Biol. Chem. 277:40735.
    5. Bartholin, L. et al. (2001) Oncogene 20:5409.
  • Entrez Gene IDs:
    10272 (Human); 83554 (Mouse)
  • Alternate Names:
    FLRG; FLRGFSRP; follistatin-like 3 (secreted glycoprotein); Follistatin-like 3; Follistatin-like protein 3; Follistatin-related gene protein; follistatin-related protein 3; FSTL3
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