Mouse Frizzled-6 Antibody

Catalog # Availability Size / Price Qty
AF1526
AF1526-SP
Detection of Mouse Frizzled-6 by Immunocytochemistry/Immunofluorescence
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Product Details
Citations (9)
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Mouse Frizzled-6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Frizzled-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Frizzled-6 is observed, and less than 1% cross-reactivity with recombinant mouse (rm) Frizzled-1, rmFrizzled-2, rmFrizzled-3, rmFrizzled-4, rmFrizzled-7, rmFrizzled-8, and rmFrizzled-9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-6
His19-Val153
Accession # Q61089
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Frizzled‑6
Flow Cytometry
2.5 µg/106 cells
Retinoic acid‑treated D3 mouse embryonic stem cell line
Immunohistochemistry
5-15 µg/mL
Immersion fixed D3 mouse embryonic stem cell line treated with retinoic acid
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry/ Immunofluorescence Detection of Mouse Frizzled-6 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Frizzled-6 by Immunocytochemistry/Immunofluorescence FZD6 protein polarity is lost in mutant tympanic ciliary epithelium.(A–B) The distribution of the core PCP protein, FZD6, was examined by immunofluorescence staining. Ciliated epithelial cells were marked with anti-acetylated-alpha -tubulin antibody (rose). (A1, A2) In the wild-type mice (at P25), FZD6 protein localized asymmetrically to the cell cortex at the level of the apical junctions (arrow in A2). (B1, B2) However, in the mutant mice (at P25), FZD6 protein lost its polarized distribution and was located on the entire membrane at the apical level (arrows in B2).(A3, B3) Schematic diagram of FZD6 protein (red line) distribution in wild-type and Spag6−/− mice. The color codes are the same as that in panel A1 and B1. Bars: A, 5 µm; B, 7.5 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25393619), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Frizzled-6 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Frizzled-6 by Immunocytochemistry/Immunofluorescence FZD6 protein polarity is lost in mutant tympanic ciliary epithelium.(A–B) The distribution of the core PCP protein, FZD6, was examined by immunofluorescence staining. Ciliated epithelial cells were marked with anti-acetylated-alpha -tubulin antibody (rose). (A1, A2) In the wild-type mice (at P25), FZD6 protein localized asymmetrically to the cell cortex at the level of the apical junctions (arrow in A2). (B1, B2) However, in the mutant mice (at P25), FZD6 protein lost its polarized distribution and was located on the entire membrane at the apical level (arrows in B2).(A3, B3) Schematic diagram of FZD6 protein (red line) distribution in wild-type and Spag6−/− mice. The color codes are the same as that in panel A1 and B1. Bars: A, 5 µm; B, 7.5 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25393619), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-6

Frizzled-6 is a member of the Frizzled/Smoothened family of G-protein coupled receptors that mediate the Wnt beta-Catenin signaling pathway.

Entrez Gene IDs
8323 (Human); 14368 (Mouse)
Alternate Names
frizzled (Drosophila) homolog 6; frizzled homolog 6 (Drosophila); Frizzled6; Frizzled-6; FZ6; fz-6; FZD6; Hfz6; seven transmembrane helix receptor

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Citations for Mouse Frizzled-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Daple deficiency causes hearing loss in adult mice by inducing defects in cochlear stereocilia and apical microtubules
    Authors: Y Ozono, A Tamura, S Nakayama, E Herawati, Y Hanada, K Ohata, M Takagishi, M Takahashi, T Imai, Y Ohta, K Oshima, T Sato, H Inohara, S Tsukita
    Scientific Reports, 2021-10-12;11(1):20224.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. EMX2-GPR156-G&alphai reverses hair cell orientation in mechanosensory epithelia
    Authors: KS Kindt, A Akturk, A Jarysta, M Day, A Beirl, M Flonard, B Tarchini
    Nature Communications, 2021-05-17;12(1):2861.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Transcriptomic analysis comparing mouse strains with extreme total lung capacities identifies novel candidate genes for pulmonary function
    Authors: L George, A Mitra, TA Thimraj, M Irmler, S Vishweswar, L Lunding, D Hühn, A Madurga, J Beckers, H Fehrenbach, S Upadhyay, H Schulz, GD Leikauf, K Ganguly
    Respir. Res., 2017-08-09;18(1):152.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Cell and Tissue Scale Forces Coregulate Fgfr2-Dependent Tetrads and Rosettes in the Mouse Embryo
    Authors: J Wen, H Tao, K Lau, H Liu, CA Simmons, Y Sun, S Hopyan
    Biophys. J., 2017-05-23;112(10):2209-2218.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. A role for core planar polarity proteins in cell contact-mediated orientation of planar cell division across the mammalian embryonic skin
    Authors: F Oozeer, LL Yates, C Dean, CJ Formstone
    Sci Rep, 2017-05-12;7(1):1880.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. The spatio-temporal domains of Frizzled6 action in planar polarity control of hair follicle orientation.
    Authors: Chang H, Smallwood P, Williams J, Nathans J
    Dev Biol, 2015-11-10;409(1):181-93.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Anisotropic stress orients remodelling of mammalian limb bud ectoderm.
    Authors: Lau K, Tao H, Liu H, Wen J, Sturgeon K, Sorfazlian N, Lazic S, Burrows J, Wong M, Li D, Deimling S, Ciruna B, Scott I, Simmons C, Henkelman R, Williams T, Hadjantonakis A, Fernandez-Gonzalez R, Sun Y, Hopyan S
    Nat Cell Biol, 2015-04-20;17(5):569-79.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Otitis media in sperm-associated antigen 6 (Spag6)-deficient mice.
    Authors: Li, Xiaofei, Xu, Lei, Li, Jianfeng, Li, Boqin, Bai, Xiaohui, Strauss, Jerome F, Zhang, Zhibing, Wang, Haibo
    PLoS ONE, 2014-11-13;9(11):e112879.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Regulation of PCDH15 function in mechanosensory hair cells by alternative splicing of the cytoplasmic domain.
    Authors: Webb SW, Grillet N, Andrade LR, Xiong W, Swarthout L, Della Santina CC, Kachar B, Muller U
    Development, 2011-04-01;138(8):1607-17.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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