Mouse Frizzled-9 Antibody

Catalog # Availability Size / Price Qty
AF2440
AF2440-SP
Product Details
Citations (2)
FAQs
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Mouse Frizzled-9 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Frizzled-9 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) Frizzled-2, rmFrizzled-3, rmFrizzled-4, rmFrizzled-6, rmFrizzled-7, and rmFrizzled-8 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Frizzled-9
Leu24-Asp186
Accession # Q9R216
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Frizzled‑9
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of embryonic mouse brain (E11)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-9

Frizzled-9 belongs to the Frizzled family of seven-transmembrane receptors, which are signaling receptors for the family of secreted Wnt ligands. The amino-terminal extracellular cysteine-rich domains of human and mouse Frizzled-9 share 94% amino acid sequence homology.

Entrez Gene IDs
8326 (Human); 14371 (Mouse)
Alternate Names
CD349 antigen; CD349; frizzled (Drosophila) homolog 9; frizzled homolog 9 (Drosophila); Frizzled9; Frizzled-9; fz-9; FZD3fzE6; FZD9; FzE6; hFz9

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Citations for Mouse Frizzled-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The developmental and genetic basis of 'clubfoot' in the peroneal muscular atrophy mutant mouse
    Authors: JM Collinson, NO Lindström, C Neves, K Wallace, C Meharg, RH Charles, ZK Ross, AM Fraser, I Mbogo, K Oras, M Nakamoto, S Barker, S Duce, Z Miedzybrod, N Vargesson
    Development, 2018-02-08;145(3):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation
    J Biol Chem, 2016-07-11;0(0):.
    Applications: Bioassay

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