Mouse IL-1 RII Antibody
Mouse IL-1 RII Antibody Summary
Accession # P27931
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IL‑1 RII in EL‑4 Mouse Cell Line by Flow Cytometry. EL-4 mouse lymphoblast cell line was stained with Goat Anti-Mouse IL-1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF563, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
IL‑1 RII in Mouse Spleen. IL-1 RII was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse IL-1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF563) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 RII
Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex. Mouse IL-1 RII shares 59% amino acid sequence homology with human IL-1 RII in their extracellular domains.
An IL-1 receptor accessory protein (1) that can heterodimerize with the type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified. This type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling type I receptor complex. Recombinant IL-1 soluble receptor type II is a potent antagonist of IL-1 action.
Citations for Mouse IL-1 RII Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Deficiency in IL-1 Receptor Type 2 Aggravates K/BxN Serum Transfer-Induced Arthritis in Mice but Has No Impact on Systemic Inflammatory Responses
Authors: P Martin, G Palmer, E Rodriguez, CA Seemayer, J Palomo, D Talabot-Ay, C Gabay
J. Immunol, 2017-02-24;0(0):.
Sample Types: Whole Tissue
ADAM17 regulates IL-1 signaling by selectively releasing IL-1 receptor type 2 from the cell surface.
Authors: Uchikawa S, Yoda M, Tohmonda T, Kanaji A, Matsumoto M, Toyama Y, Horiuchi K
Species: Primate - Chlorocebus aethiops (African Green Monkey)
Sample Types: Protein
Applications: Western Blot
Type 2 diabetes impairs insulin receptor substrate-2-mediated phosphatidylinositol 3-kinase activity in primary macrophages to induce a state of cytokine resistance to IL-4 in association with overexpression of suppressor of cytokine signaling-3.
Authors: O&apos;Connor JC, Sherry CL, Guest CB, Freund GG
J. Immunol., 2007-06-01;178(11):6886-93.
Sample Types: Peritoneal Lavage Fluid
Applications: ELISA Development
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