Mouse IL-18 R alpha /IL-1 R5 Antibody

(7 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL‑18 R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human IL‑18 R is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R alpha /IL-1 R5
    Ser19-Gly326
    Accession # Q61098
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse IL‑18 R alpha /IL‑1 R5
  • Flow Cytometry
    2.5 µg/106 cells
    Mouse splenocytes or EL-4/IL-2 cells
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in activated mouse T cells. Ahn, H.J. et al. (1997) J. Immunol. 159:2125. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑18/IL‑1F4 and 0.1 ng/mL Recombinant Mouse IL‑12.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutralization by Mouse IL‑18 R alpha /IL‑1 R5 Antibody. In the presence of Recombinant Mouse IL‑12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Mouse IL‑18/IL‑1F4 stimulates IFN‑ gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN‑ gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN‑ gamma secretion elicited by Recombinant Mouse IL‑18/IL‑1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑18 R alpha /IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF856). The ND50 is typically 0.2-0.8 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-18 R alpha/IL-1 R5

Interleukin-18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immuno-regulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL18 in vitro, coexpression of IL-18 R alpha and IL18 R beta is required for high affinity binding and IL-18 responsiveness.

Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa residues. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.

  • Long Name:
    Interleukin 18 Receptor alpha
  • Entrez Gene IDs:
    8809 (Human); 16182 (Mouse)
  • Alternate Names:
    CD218a; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL18R alpha; IL18R1; IL-18Ra
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS).
    Authors: Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA
    J. Biol. Chem., 2009;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  2. IL-18 induces a marked gene expression profile change and increased Ccl1 (I-309) production in mouse mucosal mast cell homologs.
    Authors: Wiener Z, Pocza P, Racz M, Nagy G, Tolgyesi G, Molnar V, Jaeger J, Buzas E, Gorbe E, Papp Z, Rigo J, Falus A
    Int. Immunol., 2008;20(12):1565-73.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Protective effect of IL-18 on kainate- and IL-1beta-induced cerebellar ataxia in mice.
    Authors: Andoh T, Kishi H, Motoki K, Nakanishi K, Kuraishi Y, Muraguchi A
    J. Immunol., 2008;180(4):2322-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. Interleukin-18 suppresses adiponectin expression in 3T3-L1 adipocytes via a novel signal transduction pathway involving ERK1/2-dependent NFATc4 phosphorylation.
    Authors: Chandrasekar B, Patel DN, Mummidi S, Kim JW, Clark RA, Valente AJ
    J. Biol. Chem., 2007;283(7):4200-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. Disruption of interleukin-18, but not interleukin-1, increases vulnerability to preterm delivery and fetal mortality after intrauterine inflammation.
    Authors: Wang X, Hagberg H, Mallard C, Zhu C, Hedtjarn M, Tiger CF, Eriksson K, Rosen A, Jacobsson B
    Am. J. Pathol., 2006;169(3):967-76.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  6. Combined stimulation with interleukin-18 and CpG induces murine natural killer dendritic cells to produce IFN-gamma and inhibit tumor growth.
    Authors: Chaudhry UI, Kingham TP, Plitas G, Katz SC, Raab JR, DeMatteo RP
    Cancer Res., 2006;66(21):10497-504.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis.
    Authors: Gould MP, Greene JA, Bhoj V, DeVecchio JL, Heinzel FP
    J. Immunol., 2004;172(3):1754-62.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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