Mouse IL-3 Antibody
Mouse IL-3 Antibody Summary
Accession # P01586
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑3 and Neutralization by Mouse IL‑3 Antibody. Recombinant Mouse IL-3 (Catalog # 403-ML) stimulates proliferation in the NFS60 mouse myeloid cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-3 Polyclonal Antibody (Catalog # AB-403-NA). The ND50 is typically 0.015-0.025 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated mouse mast cells, mouse keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and mouse IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on mouse cells.
IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of alpha and beta subunits. The IL-3R alpha is a member of the cytokine receptor super family and binds IL-3 with low affinity. Two distinct beta subunits, AIC2A ( beta IL-3) and AIC2B ( beta c) are present in mouse cells. beta IL-3 also binds IL-3 with low affinity and forms a high affinity receptor with the alpha subunit. The beta c subunits does not bind any cytokine but forms functional high affinity receptors with the alpha subunit of the IL-3, IL-5 and GM-CSF receptors. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.
- Yokota, T. et al., 1984, Proc. Natl. Acad. Sci. USA 81:1070.
- Fung, M.C. et al., 1984, Nature 307:233.
- Miyatake, S. et al., 1985, Proc. Natl. Acad. Sci. USA 82:316.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Mouse IL-3 Antibody
There are currently no reviews for this product. Be the first to review Mouse IL-3 Antibody and earn rewards!
Have you used Mouse IL-3 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image