Detection of Mouse LAMP‑1/CD107a by Western Blot.
Western blot shows lysates of mouse small intestine tissue and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse LAMP‑1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for LAMP‑1/CD107a at approximately 100-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of LAMP-1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse LAMP1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).View our protocol for Staining Intracellular Molecules.
LAMP1/CD107a in RAW 264.7 Mouse Cell Line.
LAMP1/CD107a was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse LAMP1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
LAMP-1 (lysosome-associated membrane glycoprotein 1; also P2B and CD107a) is a 120 kDa member of the LAMP family of proteins. It is a highly glycosylated type I transmembrane protein whose N-terminus projects either extracellularly or into the lumen of lysosomes. LAMP-1 may protect lysosomal membranes from enzymatic attack or mediate fusion of phagosomes with lysosomes. Mature mouse LAMP1 is 382 amino acids (aa) in length. It contains a 346 aa lumenal domain (aa 25‑370) and a 12 aa cytoplasmic region. Over aa 25‑370, mouse LAMP1 shares 82% and 62% aa identity with rat and human LAMP1, respectively.
Lysosome-associated Membrane Glycoprotein 1
Entrez Gene IDs:
3916 (Human); 16783 (Mouse)
CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP1; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1
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