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Mouse Lipocalin-2/NGAL Antibody

Catalog #: AF1857
60 Citations 3 Reviews Datasheet / COA / SDS
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AF1857
AF1857-SP

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Detection of Mouse Lipocalin‑2/NGAL by Western Blot.
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Citations (60)
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Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Lipocalin-2/NGAL Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Lipocalin-2/NGAL in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Lipocalin-2/NGAL
Gln21-Asn200
Accession # P11672
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
3-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Lipocalin‑2/NGAL (Catalog # 1857-LC), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Lipocalin-2/NGAL antibody by Western Blot. View Larger

Detection of Mouse Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of mouse uterus tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Lipocalin-2/NGAL at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Lipocalin-2/NGAL antibody in Mouse Mammary Gland by Immunohistochemistry (IHC-Fr). View Larger

Lipocalin‑2/NGAL in Mouse Mammary Gland. Lipocalin-2/NGAL was detected in perfusion fixed frozen sections of mouse mammary gland using Goat Anti-Mouse Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to extracellular areas in ducts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection of Mouse Lipocalin‑2/NGAL antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Lipocalin‑2/NGAL by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Lipocalin-2/NGAL at approximately 37 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipocalin-2/NGAL

Mouse Lipocalin-2 was cloned from mouse kidney cells (1). Its very high level of expression at the post-stratum uterus gave it the name uterocalin (2). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3- 5). Studies indicate that Lipocalin-2 binds a bacterial catecholate siderophore that is bound to a ferric ion, such as enterobactin, with a subnanomolar dissociation constant (Kd = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM) (6). Secretion of Lipocalin-2 in immune cells increases in response to stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing the epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).

References
  1. Hraba-Renevey, S. et al. (1989) Oncogene. 4:601. 
  2. Liu, Q. et al. (1993) Mol Reprod Dev. 46:507. 
  3. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
  4. Devireddy, L.R. et al. (2001) Science 293:829.
  5. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
  6. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
  7. Flo, T.H. et al. (2004) Nature 432:917.
  8. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
Long Name
Neutrophil Gelatinase-associated Lipocalin
Entrez Gene IDs
3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
Alternate Names
24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin

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Citations for Mouse Lipocalin-2/NGAL Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

60 Citations: Showing 1 - 10
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  1. Mammary duct luminal epithelium controls adipocyte thermogenic programme
    Authors: Patel, S;Sparman, NZR;Arneson, D;Alvarsson, A;Santos, LC;Duesman, SJ;Centonze, A;Hathaway, E;Ahn, IS;Diamante, G;Cely, I;Cho, CH;Talari, NK;Rajbhandari, AK;Goedeke, L;Wang, P;Butte, AJ;Blanpain, C;Chella Krishnan, K;Lusis, AJ;Stanley, SA;Yang, X;Rajbhandari, P;
    Nature
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC/IF
  2. Formalin-evoked pain triggers sex-specific behavior and spinal immune response
    Authors: Pepino, L;Malapert, P;Saurin, AJ;Moqrich, A;Reynders, A;
    Scientific reports
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC, ICC
  3. c-Met Mediated Cytokine Network Promotes Brain Metastasis of Breast Cancer by Remodeling Neutrophil Activities
    Authors: Liu, Y;Smith, MR;Wang, Y;D'Agostino, R;Ruiz, J;Lycan, T;Kucera, GL;Miller, LD;Li, W;Chan, MD;Farris, M;Su, J;Song, Q;Zhao, D;Chandrasekaran, A;Xing, F;
    Cancers
    Species:  Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Different Acute Kidney Injury Patterns after Renal Ischemia Reperfusion Injury and Extracorporeal Membrane Oxygenation in Mice
    Authors: R Greite, J Störmer, F Gueler, R Khalikov, A Haverich, C Kühn, N Madrahimov, R Natanov
    International Journal of Molecular Sciences, 2022-09-20;23(19):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Histone methyltransferase MLL1 drives renal tubular cell apoptosis by p53-dependent repression of E-cadherin during cisplatin-induced acute kidney injury
    Authors: C Zhang, Y Guan, J Zou, X Yang, G Bayliss, S Zhuang
    Cell Death & Disease, 2022-09-06;13(9):770.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Lipocalin-2 is an essential component of the innate immune response to Acinetobacter baumannii infection
    Authors: JR Sheldon, LE Himmel, DE Kunkle, AJ Monteith, KN Maloney, EP Skaar
    PloS Pathogens, 2022-09-02;18(9):e1010809.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  7. Myelin repair is fostered by the corticosteroid medrysone specifically acting on astroglial subpopulations
    Authors: M Silva Oliv, J Schira-Hei, L Reiche, S Han, VCM de Amorim, I Lewen, J Gruchot, P Göttle, R Akkermann, K Azim, P Küry
    EBioMedicine, 2022-08-08;83(0):104204.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: IHC
  8. Lipocalin-2 Deficiency Reduces Hepatic and Hippocampal Triggering Receptor Expressed on Myeloid Cells-2 Expressions in High-Fat Diet/Streptozotocin-Induced Diabetic Mice
    Authors: HJ Shin, Z Jin, HS An, G Park, JY Lee, SJ Lee, HM Jang, EA Jeong, KE Kim, J Lee, DY Yoo, GS Roh
    Brain sciences, 2022-07-02;12(7):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Role of lipocalin-2 in surgery-induced cognitive decline in mice: a signal from neuron to microglia
    Authors: X Xiang, X Tang, Y Yu, S Xie, L Liu, M Chen, R Zhang, X Kang, Y Zheng, G Yang, S Gan, S Zhu
    Journal of Neuroinflammation, 2022-04-12;19(1):92.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Sphingosine 1-Phosphate Receptor 5 (S1P5) Knockout Ameliorates Adenine-Induced Nephropathy
    Authors: T Eckes, S Patyna, A Koch, A Oftring, S Gauer, N Obermüller, S Schwalm, L Schaefer, J Chun, HJ Gröne, J Pfeilschif
    International Journal of Molecular Sciences, 2022-04-02;23(7):.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  11. Snapshots of nascent RNA reveal cell- and stimulus- specific responses to acute kidney injury
    Authors: TH Shen, J Stauber, K Xu, A Jacunski, N Paragas, M Callahan, R Banlengchi, AD Levitman, B Desanti de, A Beenken, MS Grau, E Mathieu, Q Zhang, Y Li, T Gopal, N Askanase, S Arumugam, S Mohan, PI Good, JS Stevens, F Lin, SK Sia, CS Lin, V D'Agati, K Kiryluk, NP Tatonetti, J Barasch
    JCI Insight, 2022-03-22;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice
    Authors: T Wan, W Zhu, Y Zhao, X Zhang, R Ye, M Zuo, P Xu, Z Huang, C Zhang, Y Xie, X Liu
    Nature Communications, 2022-03-03;13(1):1134.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  13. Pharmacological Antagonization of Cannabinoid Receptor 1 Improves Cholestasis in Abcb4-/- Mice
    Authors: N Helmrich, M Roderfeld, A Baier, A Windhorst, D Herebian, E Mayatepek, C Dierkes, M Ocker, D Glebe, B Christ, Y Churin, K Irungbam, E Roeb
    Cellular and Molecular Gastroenterology and Hepatology, 2021-12-23;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis
    Authors: F Yao, Y Deng, Y Zhao, Y Mei, Y Zhang, X Liu, C Martinez, X Su, RR Rosato, H Teng, Q Hang, S Yap, D Chen, Y Wang, MM Chen, M Zhang, H Liang, D Xie, X Chen, H Zhu, JC Chang, MJ You, Y Sun, B Gan, L Ma
    Nature Communications, 2021-12-17;12(1):7333.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Farrerol Ameliorated Cisplatin-Induced Chronic Kidney Disease Through Mitophagy Induction via Nrf2/PINK1 Pathway
    Authors: N Ma, Z Wei, J Hu, W Gu, X Ci
    Frontiers in Pharmacology, 2021-11-11;12(0):768700.
    Species: Mouse, Transgenic Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  16. DSS-induced inflammation in the colon drives a proinflammatory signature in the brain that is ameliorated by prophylactic treatment with the S100A9 inhibitor paquinimod
    Authors: S Talley, R Valiauga, L Anderson, AR Cannon, MA Choudhry, EM Campbell
    Journal of Neuroinflammation, 2021-11-10;18(1):263.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  17. Chronic cerebral lipocalin 2 exposure elicits hippocampal neuronal dysfunction and cognitive impairment
    Authors: B Olson, X Zhu, MA Norgard, P Diba, PR Levasseur, AC Buenafe, C Huisman, KG Burfeind, KA Michaelis, G Kong, T Braun, DL Marks
    Brain, Behavior, and Immunity, 2021-07-08;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. NLRP3 Triggers Attenuate Lipocalin-2 Expression Independent with Inflammasome Activation
    Authors: H Ahn, G Lee, J Kim, J Park, SG Kang, SI Yoon, E Lee, GS Lee
    Cells, 2021-07-02;10(7):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Lipocalin 2 mediates appetite suppression during pancreatic cancer cachexia
    Authors: B Olson, X Zhu, MA Norgard, PR Levasseur, JT Butler, A Buenafe, KG Burfeind, KA Michaelis, KR Pelz, H Mendez, J Edwards, SM Krasnow, AJ Grossberg, DL Marks
    Nature Communications, 2021-04-06;12(1):2057.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Oxysterols present in Alzheimer's disease brain induce synaptotoxicity by activating astrocytes: A major role for lipocalin-2
    Authors: E Staurenghi, V Cerrato, P Gamba, G Testa, S Giannelli, V Leoni, C Caccia, A Buffo, W Noble, BG Perez-Niev, G Leonarduzz
    Redox Biology, 2020-12-17;39(0):101837.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  21. Lipocalin 2 induces neuroinflammation and blood-brain barrier dysfunction through liver-brain axis in murine model of nonalcoholic steatohepatitis
    Authors: A Mondal, D Bose, P Saha, S Sarkar, R Seth, D Kimono, M Albadrani, M Nagarkatti, P Nagarkatti, S Chatterjee
    J Neuroinflammation, 2020-07-04;17(1):201.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Dysregulated mesenchymal PDGFR-&beta drives kidney fibrosis
    Authors: EM Buhl, S Djudjaj, BM Klinkhamme, K Ermert, VG Puelles, MT Lindenmeye, CD Cohen, C He, E Borkham-Ka, R Weiskirche, B Denecke, P Trairatphi, J Saez-Rodri, TB Huber, LE Olson, J Floege, P Boor
    EMBO Mol Med, 2020-01-14;0(0):e11021.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Lipocalin-2 Regulates Epidermal Growth Factor Receptor Intracellular Trafficking
    Authors: L Yammine, A Zablocki, W Baron, F Terzi, M Gallazzini
    Cell Rep, 2019-11-12;29(7):2067-2077.e6.
    Species: Human
    Sample Types: Cell Lysate
    Applications: IP
  24. Sex-specific metabolic functions of adipose Lipocalin-2
    Authors: K Chella Kri, S Sabir, M Shum, Y Meng, R Acín-Pérez, JM Lang, RR Floyd, L Vergnes, MM Seldin, BK Fuqua, DW Jayasekera, SK Nand, DC Anum, C Pan, L Stiles, M Péterfy, K Reue, M Liesa, AJ Lusis
    Mol Metab, 2019-09-27;30(0):30-47.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  25. Secretomes from metastatic breast cancer cells, enriched for a prognostically unfavorable LCN2 axis, induce anti-inflammatory MSC actions and a tumor-supportive premetastatic lung
    Authors: KJ Meade, F Sanchez, A Aguayo, N Nadales, SG Hamalian, TL Uhlendorf, LR Banner, JA Kelber
    Oncotarget, 2019-04-30;10(32):3027-3039.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Negr1 controls adult hippocampal neurogenesis and affective behaviors
    Authors: K Noh, H Lee, TY Choi, Y Joo, SJ Kim, H Kim, JY Kim, JW Jahng, S Lee, SY Choi, SJ Lee
    Mol. Psychiatry, 2019-01-16;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  27. CXCL12 and MYC control energy metabolism to support adaptive responses after kidney injury
    Authors: TA Yakulov, AP Todkar, K Slanchev, J Wiegel, A Bona, M Gro beta, A Scholz, I Hess, A Wurditsch, F Grahammer, TB Huber, V Lecaudey, T Bork, J Hochrein, M Boerries, J Leenders, P de Tullio, F Jouret, A Kramer-Zuc, G Walz
    Nat Commun, 2018-09-10;9(1):3660.
    Species: Mouse
    Sample Types: Urine
    Applications: Western Blot
  28. The IgCAM CLMP is required for intestinal and ureteral smooth muscle contraction by regulating Connexin43 and 45 expression in mice
    Authors: H Langhorst, R Jüttner, D Groneberg, A Mohtashamd, L Pelz, B Purfürst, KM Schmidt-Ot, A Friebe, FG Rathjen
    Dis Model Mech, 2018-02-22;0(0):.
    Species: Mouse
    Sample Types: Urine
    Applications: Western Blot
  29. Early Gene Expression Profile in Retinal Ganglion Cell Layer After Optic Nerve Crush in Mice
    Authors: S Ueno, A Yoneshige, Y Koriyama, M Hagiyama, Y Shimomura, A Ito
    Invest. Ophthalmol. Vis. Sci., 2018-01-01;59(1):370-380.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  30. Elevated urinary CRELD2 is associated with endoplasmic reticulum stress-mediated kidney disease
    Authors: Y Kim, SJ Park, SR Manson, CA Molina, K Kidd, H Thiessen-P, RJ Perry, H Liapis, S Kmoch, CR Parikh, AJ Bleyer, YM Chen
    JCI Insight, 2017-12-07;2(23):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  31. Early involvement of cellular stress and inflammatory signals in the pathogenesis of tubulointerstitial kidney disease due to UMOD mutations
    Authors: M Trudu, C Schaeffer, M Riba, M Ikehata, P Brambilla, P Messa, F Martinelli, MP Rastaldi, L Rampoldi
    Sci Rep, 2017-08-07;7(1):7383.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  32. Immune-induced fever is dependent on local but not generalized prostaglandin E2 synthesis in the brain
    Authors: A Eskilsson, T Matsuwaki, K Shionoya, E Mirrasekhi, J Zajdel, M Schwaninge, D Engblom, A Blomqvist
    J. Neurosci., 2017-04-24;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  33. MC4R-dependent suppression of appetite by bone-derived lipocalin 2
    Authors: I Mosialou, S Shikhel, JM Liu, A Maurizi, N Luo, Z He, Y Huang, H Zong, RA Friedman, J Barasch, P Lanzano, L Deng, RL Leibel, M Rubin, T Nicholas, W Chung, LM Zeltser, KW Williams, JE Pessin, S Kousteni
    Nature, 2017-03-08;543(7645):385-390.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  34. Cyclooxygenase Isoform Exchange Blocks Brain-Mediated Inflammatory Symptoms
    PLoS ONE, 2016-11-18;11(11):e0166153.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  35. Keap1 hypomorphism protects against ischemic and obstructive kidney disease
    Sci Rep, 2016-11-02;6(0):36185.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  36. Beneficial Effects of Sodium Phenylbutyrate Administration During Infection with Salmonella enterica serovar Typhimurium
    Infect Immun, 2016-08-19;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  37. Influenza Virus Affects Intestinal Microbiota and Secondary Salmonella Infection in the Gut through Type I Interferons
    PLoS Pathog, 2016-05-05;12(5):e1005572.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  38. A novel role for protein tyrosine phosphatase 1B as a positive regulator of neuroinflammation
    Authors: GJ Song, M Jung, JH Kim, H Park, MH Rahman, S Zhang, ZY Zhang, DH Park, H Kook, IK Lee, K Suk
    J Neuroinflammation, 2016-04-19;13(1):86.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  39. Identification of lipocalin-2 as a PKCdelta phosphorylation substrate in neutrophils.
    Authors: Weng Y, Wang G, Messing R, Chou W
    J Biomed Sci, 2015-03-20;22(0):21.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  40. Lipocalin 2: a new mechanoresponding gene regulating bone homeostasis.
    Authors: Rucci N, Capulli M, Piperni S, Cappariello A, Lau P, Frings-Meuthen P, Heer M, Teti A
    J Bone Miner Res, 2015-02-01;30(2):357-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Estrogen receptor (ER)alpha-regulated lipocalin 2 expression in adipose tissue links obesity with breast cancer progression.
    Authors: Drew B, Hamidi H, Zhou Z, Villanueva C, Krum S, Calkin A, Parks B, Ribas V, Kalajian N, Phun J, Daraei P, Christofk H, Hewitt S, Korach K, Tontonoz P, Lusis A, Slamon D, Hurvitz S, Hevener A
    J Biol Chem, 2014-12-02;290(9):5566-81.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  42. Lipocalin 2 is a regulator of macrophage polarization and NF-kappaB/STAT3 pathway activation.
    Authors: Guo H, Jin D, Chen X
    Mol Endocrinol, 2014-08-15;28(10):1616-28.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  43. mTORC1 maintains renal tubular homeostasis and is essential in response to ischemic stress.
    Authors: Grahammer F, Haenisch N, Steinhardt F, Sander L, Roerden M, Arnold F, Cordts T, Wanner N, Reichardt W, Kerjaschki D, Ruegg M, Hall M, Moulin P, Busch H, Boerries M, Walz G, Artunc F, Huber T
    Proc Natl Acad Sci U S A, 2014-06-23;111(27):E2817-26.
    Species: Mouse
    Sample Types: Urine
    Applications: Western Blot
  44. alpha-Intercalated cells defend the urinary system from bacterial infection.
    Authors: Paragas N, Kulkarni R, Werth M, Schmidt-Ott K, Forster C, Deng R, Zhang Q, Singer E, Klose A, Shen T, Francis K, Ray S, Vijayakumar S, Seward S, Bovino M, Xu K, Takabe Y, Amaral F, Mohan S, Wax R, Corbin K, Sanna-Cherchi S, Mori K, Johnson L, Nickolas T, D'Agati V, Lin C, Qiu A, Al-Awqati Q, Ratner A, Barasch J
    J Clin Invest, 2014-06-17;124(7):2963-76.
    Species: Mouse
    Sample Types: Urine
    Applications: Western Blot
  45. Lipocalin 2 regulates brown fat activation via a nonadrenergic activation mechanism.
    Authors: Zhang Y, Guo H, Deis J, Mashek M, Zhao M, Ariyakumar D, Armien A, Bernlohr D, Mashek D, Chen X
    J Biol Chem, 2014-06-10;289(32):22063-77.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  46. Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2.
    Authors: Miao Q, Ku A, Nishino Y, Howard J, Rao A, Shaver T, Garcia G, Le D, Karlin K, Westbrook T, Poli V, Nguyen H
    Nat Commun, 2014-06-09;5(0):4088.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, Neutralization
  47. Human alpha-defensin expression is not dependent on CCAAT/enhancer binding protein-epsilon in a murine model.
    Authors: Glenthoj A, Dahl S, Larsen M, Cowland J, Borregaard N
    PLoS ONE, 2014-03-21;9(3):e92471.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  48. The cytokine IL-22 promotes pathogen colonization by suppressing related commensal bacteria.
    Authors: Behnsen J, Jellbauer S, Wong C, Edwards R, George M, Ouyang W, Raffatellu M
    Immunity, 2014-02-06;40(2):262-73.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  49. The bacteriostatic protein lipocalin 2 is induced in the central nervous system of mice with west Nile virus encephalitis.
    Authors: Nocon A, Ip J, Terry R, Lim S, Getts D, Muller M, Hofer M, King N, Campbell I
    J Virol, 2013-10-30;88(1):679-89.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  50. In vivo implanted bone marrow-derived mesenchymal stem cells trigger a cascade of cellular events leading to the formation of an ectopic bone regenerative niche.
    Authors: Tasso, Roberta, Ulivi, Valentin, Reverberi, Daniele, Lo Sicco, Claudia, Descalzi, Fiorella, Cancedda, Ranieri
    Stem Cells Dev, 2013-09-14;22(24):3178-91.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  51. Role of lipocalin-2-chemokine axis in the development of neuropathic pain following peripheral nerve injury.
    Authors: Jeon, Sangmin, Jha, Mithiles, Ock, Jiyeon, Seo, Jungwan, Jin, Myungwon, Cho, Heejung, Lee, Won-Ha, Suk, Kyoungho
    J Biol Chem, 2013-07-08;288(33):24116-27.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  52. Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
    Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
    Biomark Insights, 2012-07-10;7(0):87-104.
    Species: Mouse
    Sample Types: BALF
    Applications: Western Blot
  53. No effect of NGAL/lipocalin-2 on aggressiveness of cancer in the MMTV-PyMT/FVB/N mouse model for breast cancer.
    Authors: Cramer EP, Glenthoj A, Hager M, Juncker-Jensen A, Engelholm LH, Santoni-Rugiu E, Lund LR, Laerum OD, Cowland JB, Borregaard N
    PLoS ONE, 2012-06-21;7(6):e39646.
    Species: Mouse
    Sample Types: Cell Lysates, Plasma, Whole Tissue
    Applications: ELISA Development, IHC-P, Western Blot
  54. Transcriptional profiling of stroma from inflamed and resting lymph nodes defines immunological hallmarks.
    Authors: Malhotra D, Fletcher AL, Astarita J, Lukacs-Kornek V, Tayalia P, Gonzalez SF, Elpek KG, Chang SK, Knoblich K, Hemler ME, Brenner MB, Carroll MC, Mooney DJ, Turley SJ, Zhou Y, Shinton SA, Hardy RR, Bezman NA, Sun JC, Kim CC, Lanier LL, Miller J, Brown B, Merad M, Fletcher AL, Elpek KG, Bellemare-Pelletier A, Malhotra D, Turley SJ, Narayan K, Sylvia K, Kang J, Gazit R, Garrison B, Rossi DJ, Jojic V, Koller D, Jianu R, Laidlaw D, Costello J, Collins J, Cohen N, Brennan P, Brenner MB, Shay T, Regev A, Francis Kim TN, Rao A, Wagers EL, Gautier C, Jakubzick GJ, Randolph P, Monach AJ, Best J, Knell A, Goldrath T, Heng T, Kreslavsky M, Painter D, Mathis C, Benoist
    Nat. Immunol., 2012-04-01;13(5):499-510.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  55. Lipocalin 2 in the central nervous system host response to systemic lipopolysaccharide administration.
    Authors: Ip JP, Nocon AL, Hofer MJ, Lim SL, Muller M, Campbell IL
    J Neuroinflammation, 2011-09-26;8(0):124.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  56. ER stress drives Lipocalin 2 upregulation in prostate cancer cells in an NF-kappaB-dependent manner.
    Authors: Mahadevan NR, Rodvold J, Almanza G, Perez AF, Wheeler MC, Zanetti M
    BMC Cancer, 2011-06-07;11(0):229.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  57. Lipocalin-2 is an autocrine mediator of reactive astrocytosis.
    Authors: Lee S, Park JY, Lee WH, Kim H, Park HC, Mori K, Suk K
    J. Neurosci., 2009-01-07;29(1):234-49.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  58. Urinary neutrophil gelatinase-associated lipocalin levels reflect damage to glomeruli, proximal tubules, and distal nephrons.
    Authors: Kuwabara T, Mori K, Mukoyama M, Kasahara M, Yokoi H, Saito Y, Yoshioka T, Ogawa Y, Imamaki H, Kusakabe T, Ebihara K, Omata M, Satoh N, Sugawara A, Barasch J, Nakao K
    Kidney Int., 2008-10-01;75(3):285-94.
    Species: Mouse
    Sample Types: Urine, Whole Tissue
    Applications: IHC-P, Western Blot
  59. The adipokine lipocalin 2 is regulated by obesity and promotes insulin resistance.
    Authors: Yan QW, Yang Q, Mody N, Graham TE, Hsu CH, Xu Z, Houstis NE, Kahn BB, Rosen ED
    Diabetes, 2007-07-16;56(10):2533-40.
    Species: Mouse
    Sample Types: Plasma
    Applications: Western Blot
  60. Inhalation of ultrafine carbon particles triggers biphasic pro-inflammatory response in the mouse lung.
    Authors: Andre E, Stoeger T, Takenaka S, Bahnweg M, Ritter B, Karg E, Lentner B, Reinhard C, Schulz H, Wjst M
    Eur. Respir. J., 2006-04-26;28(2):275-85.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

FAQs

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Isotype Controls

Normal Goat IgG Control

AB-108-C
377 Citations
20 Reviews
1 Image
Ctrl

Reconstitution Buffers

Reconstitution Buffer 1 (PBS)

RB01
3 Reviews

Secondary Antibodies

Chicken Anti-Goat IgG Biotinylated Antibody

BAF019
WB

Donkey Anti-Goat IgG Antibody

AF109
8 Citations
WB

Donkey Anti-Goat IgG Biotinylated Antibody

BAF109
3 Citations
1 Review
WB

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

NL003
8 Citations
Flow,IHC,ICC

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

NL001
28 Citations
2 Reviews
Flow,IHC,ICC

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

NL002
5 Citations
Flow,IHC,ICC

Goat IgG (H+L) APC-conjugated Antibody

F0108
14 Citations
1 Image
Flow

Goat IgG (H+L) Antibody

R-401-C-ABS
WB,ELISA

Goat IgG (H+L) Fluorescein-conjugated Antibody

F0109
3 Citations
1 Image
Flow

Goat IgG (H+L) PE-conjugated Antibody

F0107
11 Citations
2 Reviews
1 Image
Flow

Goat IgG (H+L) PerCP-conjugated Antibody

F0124
Flow

Goat IgG HRP-conjugated Antibody

HAF017
58 Citations
10 Reviews
1 Image
WB

Goat IgG HRP-conjugated Antibody

HAF109
51 Citations
4 Reviews
2 Images
WB,Simple Western

Goat IgG Horseradish Peroxidase-conjugated Antibody

HAF019
21 Citations
2 Images
WB,IHC

Goat IgG VisUCyte HRP Polymer

VC004
8 Citations
1 Image
IHC

Rabbit Anti-Goat IgG Biotinylated Antibody

BAF017
2 Citations
1 Review
WB

Reviews for Mouse Lipocalin-2/NGAL Antibody

Average Rating: 5 (Based on 3 Reviews)

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Mouse Lipocalin-2/NGAL Antibody
By Anonymous on 11/06/2020
Application: ICC/IF Sample Tested: Brain tissue Species: Mouse
Immunocytochemistry/Immunofluorescence Mouse Lipocalin-2/NGAL Antibody AF1857
Mouse Lipocalin-2/NGAL Antibody
By Anonymous on 02/16/2018
Application: ICC/IF Sample Tested: Differentiated osteoblasts Species: Mouse

incubated O/N with AF-1857 (1:500) at 4oC.

Immunocytochemistry/Immunofluorescence Mouse Lipocalin-2/NGAL Antibody AF1857
Mouse Lipocalin-2/NGAL Antibody
By Anonymous on 01/06/2017
Application: WB Sample Tested: Kidney tissue Species: Mouse
Western Blot Mouse Lipocalin-2/NGAL Antibody AF1857