Mouse Lipocalin-2/NGAL Antibody

(29 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Lipocalin-2/NGAL in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant rat Lipocalin-2/NGAL is observed, approximately 10% cross-reactivity with recombinant human (rh) Lipocalin-2/NGAL is observed, and less than 5% cross-reactivity with rhLipocalin-1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Lipocalin-2/NGAL
    Gln21-Asn200
    Accession # P11672
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of mouse uterus tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for
Lipocalin‑2/NGAL at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Lipocalin‑2/NGAL by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Lipocalin‑2/NGAL at approximately 37 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse
Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lipocalin-2/NGAL
Mouse Lipocalin-2 was cloned from mouse kidney cells (1). Its very high level of expression at the post-stratum uterus gave it the name uterocalin (2). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3- 5). Studies indicate that Lipocalin-2 binds a bacterial catecholate siderophore that is bound to a ferric ion, such as enterobactin, with a subnanomolar dissociation constant (Kd = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM) (6). Secretion of Lipocalin-2 in immune cells increases in response to stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing the epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).
  • References:
    1. Hraba-Renevey, S. et al. (1989) Oncogene. 4:601. 
    2. Liu, Q. et al. (1993) Mol Reprod Dev. 46:507. 
    3. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
    4. Devireddy, L.R. et al. (2001) Science 293:829.
    5. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
    6. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
    7. Flo, T.H. et al. (2004) Nature 432:917.
    8. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
  • Long Name:
    Neutrophil Gelatinase-associated Lipocalin
  • Entrez Gene IDs:
    3934 (Human); 16819 (Mouse); 170496 (Rat)
  • Alternate Names:
    24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. MC4R-dependent suppression of appetite by bone-derived lipocalin 2
    Authors: I Mosialou, S Shikhel, JM Liu, A Maurizi, N Luo, Z He, Y Huang, H Zong, RA Friedman, J Barasch, P Lanzano, L Deng, RL Leibel, M Rubin, T Nicholas, W Chung, LM Zeltser, KW Williams, JE Pessin, S Kousteni
    Nature, 2017;543(7645):385-390.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Immune-induced fever is dependent on local but not generalized prostaglandin E2 synthesis in the brain
    Authors: A Eskilsson, T Matsuwaki, K Shionoya, E Mirrasekhi, J Zajdel, M Schwaninge, D Engblom, A Blomqvist
    J. Neurosci., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Early involvement of cellular stress and inflammatory signals in the pathogenesis of tubulointerstitial kidney disease due to UMOD mutations
    Authors: M Trudu, C Schaeffer, M Riba, M Ikehata, P Brambilla, P Messa, F Martinelli, MP Rastaldi, L Rampoldi
    Sci Rep, 2017;7(1):7383.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. A novel role for protein tyrosine phosphatase 1B as a positive regulator of neuroinflammation
    Authors: GJ Song, M Jung, JH Kim, H Park, MH Rahman, S Zhang, ZY Zhang, DH Park, H Kook, IK Lee, K Suk
    J Neuroinflammation, 2016;13(1):86.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  5. Cyclooxygenase Isoform Exchange Blocks Brain-Mediated Inflammatory Symptoms
    PLoS ONE, 2016;11(11):e0166153.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  6. Beneficial Effects of Sodium Phenylbutyrate Administration During Infection with Salmonella enterica serovar Typhimurium
    Infect Immun, 2016;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  7. Keap1 hypomorphism protects against ischemic and obstructive kidney disease
    Sci Rep, 2016;6(0):36185.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  8. Influenza Virus Affects Intestinal Microbiota and Secondary Salmonella Infection in the Gut through Type I Interferons
    PLoS Pathog, 2016;12(5):e1005572.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  9. Lipocalin 2: a new mechanoresponding gene regulating bone homeostasis.
    Authors: Rucci N, Capulli M, Piperni S, Cappariello A, Lau P, Frings-Meuthen P, Heer M, Teti A
    J Bone Miner Res, 2015;30(2):357-68.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  10. Identification of lipocalin-2 as a PKCdelta phosphorylation substrate in neutrophils.
    Authors: Weng Y, Wang G, Messing R, Chou W
    J Biomed Sci, 2015;22(0):21.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  11. Estrogen receptor (ER)alpha-regulated lipocalin 2 expression in adipose tissue links obesity with breast cancer progression.
    Authors: Drew B, Hamidi H, Zhou Z, Villanueva C, Krum S, Calkin A, Parks B, Ribas V, Kalajian N, Phun J, Daraei P, Christofk H, Hewitt S, Korach K, Tontonoz P, Lusis A, Slamon D, Hurvitz S, Hevener A
    J Biol Chem, 2015;290(9):5566-81.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  12. mTORC1 maintains renal tubular homeostasis and is essential in response to ischemic stress.
    Authors: Grahammer F, Haenisch N, Steinhardt F, Sander L, Roerden M, Arnold F, Cordts T, Wanner N, Reichardt W, Kerjaschki D, Ruegg M, Hall M, Moulin P, Busch H, Boerries M, Walz G, Artunc F, Huber T
    Proc Natl Acad Sci U S A, 2014;111(27):E2817-26.
    Species: Mouse
    Sample Type: Urine
    Application: WB
  13. Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2.
    Authors: Miao Q, Ku A, Nishino Y, Howard J, Rao A, Shaver T, Garcia G, Le D, Karlin K, Westbrook T, Poli V, Nguyen H
    Nat Commun, 2014;5(0):4088.
  14. alpha-Intercalated cells defend the urinary system from bacterial infection.
    Authors: Paragas N, Kulkarni R, Werth M, Schmidt-Ott K, Forster C, Deng R, Zhang Q, Singer E, Klose A, Shen T, Francis K, Ray S, Vijayakumar S, Seward S, Bovino M, Xu K, Takabe Y, Amaral F, Mohan S, Wax R, Corbin K, Sanna-Cherchi S, Mori K, Johnson L, Nickolas T, D'Agati V, Lin C, Qiu A, Al-Awqati Q, Ratner A, Barasch J
    J Clin Invest, 2014;124(7):2963-76.
    Species: Mouse
    Sample Type: Urine
    Application: WB
  15. The cytokine IL-22 promotes pathogen colonization by suppressing related commensal bacteria.
    Authors: Behnsen J, Jellbauer S, Wong C, Edwards R, George M, Ouyang W, Raffatellu M
    Immunity, 2014;40(2):262-73.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  16. Human alpha-defensin expression is not dependent on CCAAT/enhancer binding protein-epsilon in a murine model.
    Authors: Glenthoj A, Dahl S, Larsen M, Cowland J, Borregaard N
    PLoS ONE, 2014;9(3):e92471.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  17. The bacteriostatic protein lipocalin 2 is induced in the central nervous system of mice with west Nile virus encephalitis.
    Authors: Nocon A, Ip J, Terry R, Lim S, Getts D, Muller M, Hofer M, King N, Campbell I
    J Virol, 2014;88(1):679-89.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  18. Lipocalin 2 is a regulator of macrophage polarization and NF-kappaB/STAT3 pathway activation.
    Authors: Guo H, Jin D, Chen X
    Mol Endocrinol, 2014;28(10):1616-28.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  19. Lipocalin 2 regulates brown fat activation via a nonadrenergic activation mechanism.
    Authors: Zhang Y, Guo H, Deis J, Mashek M, Zhao M, Ariyakumar D, Armien A, Bernlohr D, Mashek D, Chen X
    J Biol Chem, 2014;289(32):22063-77.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  20. In vivo implanted bone marrow-derived mesenchymal stem cells trigger a cascade of cellular events leading to the formation of an ectopic bone regenerative niche.
    Authors: Tasso, Roberta, Ulivi, Valentin, Reverberi, Daniele, Lo Sicco, Claudia, Descalzi, Fiorella, Cancedda, Ranieri
    Stem Cells Dev, 2013;22(24):3178-91.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  21. Transcriptional profiling of stroma from inflamed and resting lymph nodes defines immunological hallmarks.
    Authors: Malhotra D, Fletcher AL, Astarita J, Lukacs-Kornek V, Tayalia P, Gonzalez SF, Elpek KG, Chang SK, Knoblich K, Hemler ME, Brenner MB, Carroll MC, Mooney DJ, Turley SJ, Zhou Y, Shinton SA, Hardy RR, Bezman NA, Sun JC, Kim CC, Lanier LL, Miller J, Brown B, Merad M, Fletcher AL, Elpek KG, Bellemare-Pelletier A, Malhotra D, Turley SJ, Narayan K, Sylvia K, Kang J, Gazit R, Garrison B, Rossi DJ, Jojic V, Koller D, Jianu R, Laidlaw D, Costello J, Collins J, Cohen N, Brennan P, Brenner MB, Shay T, Regev A, Francis Kim TN, Rao A, Wagers EL, Gautier C, Jakubzick GJ, Randolph P, Monach AJ, Best J, Knell A, Goldrath T, Heng T, Kreslavsky M, Painter D, Mathis C, Benoist
    Nat. Immunol., 2012;13(5):499-510.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  22. Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
    Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
    Biomark Insights, 2012;7(0):87-104.
    Species: Mouse
    Sample Type: BALF
    Application: WB
  23. No effect of NGAL/lipocalin-2 on aggressiveness of cancer in the MMTV-PyMT/FVB/N mouse model for breast cancer.
    Authors: Cramer EP, Glenthoj A, Hager M, Juncker-Jensen A, Engelholm LH, Santoni-Rugiu E, Lund LR, Laerum OD, Cowland JB, Borregaard N
    PLoS ONE, 2012;7(6):e39646.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  24. Lipocalin 2 in the central nervous system host response to systemic lipopolysaccharide administration.
    Authors: Ip JP, Nocon AL, Hofer MJ, Lim SL, Muller M, Campbell IL
    J Neuroinflammation, 2011;8(0):124.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  25. ER stress drives Lipocalin 2 upregulation in prostate cancer cells in an NF-kappaB-dependent manner.
    Authors: Mahadevan NR, Rodvold J, Almanza G, Perez AF, Wheeler MC, Zanetti M
    BMC Cancer, 2011;11(0):229.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  26. Lipocalin-2 is an autocrine mediator of reactive astrocytosis.
    Authors: Lee S, Park JY, Lee WH, Kim H, Park HC, Mori K, Suk K
    J. Neurosci., 2009;29(1):234-49.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  27. Urinary neutrophil gelatinase-associated lipocalin levels reflect damage to glomeruli, proximal tubules, and distal nephrons.
    Authors: Kuwabara T, Mori K, Mukoyama M, Kasahara M, Yokoi H, Saito Y, Yoshioka T, Ogawa Y, Imamaki H, Kusakabe T, Ebihara K, Omata M, Satoh N, Sugawara A, Barasch J, Nakao K
    Kidney Int., 2009;75(3):285-94.
    Species: Mouse
    Sample Type: Urine
    Application: WB
  28. The adipokine lipocalin 2 is regulated by obesity and promotes insulin resistance.
    Authors: Yan QW, Yang Q, Mody N, Graham TE, Hsu CH, Xu Z, Houstis NE, Kahn BB, Rosen ED
    Diabetes, 2007;56(10):2533-40.
    Species: Mouse
    Sample Type: Plasma
    Application: WB
  29. Inhalation of ultrafine carbon particles triggers biphasic pro-inflammatory response in the mouse lung.
    Authors: Andre E, Stoeger T, Takenaka S, Bahnweg M, Ritter B, Karg E, Lentner B, Reinhard C, Schulz H, Wjst M
    Eur. Respir. J., 2006;28(2):275-85.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
Expand to show all 29 Citations
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 application: Western Blot.

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Western Blot Mouse Lipocalin-2/NGAL Antibody AF1857
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 WB Mouse Anonymous 01/06/2017
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Western Blot Mouse Lipocalin-2/NGAL Antibody AF1857
Western Blot: Mouse Lipocalin-2/NGAL Antibody [AF1857]

Summary

ApplicationWestern Blot
Sample TestedKidney tissue
SpeciesMouse

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