Mouse M-CSF Antibody
Mouse M-CSF Antibody Summary
Accession # Q3U4F9
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by M‑CSF and Neutralization by Mouse M‑CSF Antibody. Recombinant Mouse M-CSF (Catalog # 416-ML) stimulates proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse M-CSF (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse M-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF416). The ND50 is typically 0.015-0.035 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells, and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O-glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82%, and 81% aa identity with corresponding regions of rat, dog, cow, and human M-CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.
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- Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
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- Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.
- Nandi, S. et al. (2006) Blood 107:786.
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- Suzu, S. et al. (1992) J. Biol. Chem. 267:16812.
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- Koths, K. (1997) Mol. Reprod. Dev. 46:31.
- Jang, M-H. et al. (2006) J. Immunol. 177:4055.
- DeLamarter, J.F. et al. (1987) Nucleic Acids Res. 15:2389.
- Ladner, M.B. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6706.
Citations for Mouse M-CSF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 6
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Activation of neuronal FLT3 promotes exaggerated sensorial and emotional pain-related behaviors facilitating the transition from acute to chronic pain
Authors: A Tassou, M Thouaye, D Gilabert, A Jouvenel, JP Leyris, C Sonrier, L Diouloufet, I Mechaly, S Mallié, J Bertin, M Chentouf, M Neiveyans, M Pugnière, P Martineau, B Robert, X Capdevila, J Valmier, C Rivat
Progress in neurobiology, 2023-01-13;0(0):102405.
Sample Types: Whole Tissue
Diet-induced inflammation in the anterior paraventricular thalamus induces compulsive sucrose-seeking
Authors: J Cheng, X Ma, C Li, R Ullah, X Wang, J Long, Z Yuan, S Liu, J Fu, Z Chen, Y Shen, YD Zhou
Nature Neuroscience, 2022-08-01;25(8):1009-1013.
Sample Types: In Vivo
Applications: In Vivo
Microglial responses to CSF1 overexpression do not promote the expansion of other glial lineages
Authors: I De, V Maklakova, S Litscher, MM Boyd, LC Klemm, Z Wang, C Kendziorsk, LS Collier
Journal of Neuroinflammation, 2021-07-19;18(1):162.
Sample Types: Whole Tissue
Microglia Are Indispensable for Synaptic Plasticity in the Spinal Dorsal Horn and Chronic Pain
Authors: LJ Zhou, J Peng, YN Xu, WJ Zeng, J Zhang, X Wei, CL Mai, ZJ Lin, Y Liu, M Murugan, UB Eyo, AD Umpierre, WJ Xin, T Chen, M Li, H Wang, JR Richardson, Z Tan, XG Liu, LJ Wu
Cell Rep, 2019-06-25;27(13):3844-3859.e6.
Sample Types: In Vivo, Tissue Homogenates, Whole Cells, Whole Tissue
Applications: ICC, IHC, Neutralization, Western Blot
Microglial pannexin-1 channel activation is a spinal determinant of joint pain
Authors: M Mousseau, NE Burma, KY Lee, H Leduc-Pess, CHT Kwok, AR Reid, M O'Brien, B Sagalajev, JA Stratton, N Patrick, PL Stemkowski, J Biernaskie, GW Zamponi, P Salo, JJ McDougall, SA Prescott, JR Matyas, T Trang
Sci Adv, 2018-08-08;4(8):eaas9846.
Sample Types: Tissue Homogenates
Applications: Western Blot
Bacterial lipopolysaccharide induces osteoclast formation in RAW 264.7 macrophage cells.
Authors: Islam S, Hassan F, Tumurkhuu G, Dagvadorj J, Koide N, Naiki Y, Mori I, Yoshida T, Yokochi T
Biochem. Biophys. Res. Commun., 2007-06-12;360(2):346-51.
Sample Types: Cell Lysates
Applications: Western Blot
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