Mouse MAdCAM-1 Antibody

Catalog # Availability Size / Price Qty
AF993
AF993-SP
Detection of Mouse MAdCAM‑1 by Western Blot.
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Mouse MAdCAM-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MAdCAM-1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) MAdCAM-1, recombinant mouse (rm) ICAM-1, rmICAM-2, rmICAM-5, rmVCAM-1, and rhICAM-3.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MAdCAM-1
Gln22-Thr365, Predicted
Accession # NP_038619
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse MAdCAM-1 antibody by Western Blot. View Larger

Detection of Mouse MAdCAM‑1 by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MAdCAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF993) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MAdCAM-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry MAdCAM-1 antibody in Mouse Intestine by Immunohistochemistry (IHC-Fr). View Larger

MAdCAM‑1 in Mouse Intestine. MAdCAM-1 was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse MAdCAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF993) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the endothelial cells of blood capillaries in intestinal villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MAdCAM-1

Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is an immunoglobulin (Ig) cell adhesion molecule family member. In addition to Ig domains, it contains a mucin-like domain and a membrane proximal domain with similarity to IgA. MAdCAM-1 is involved in lymphocyte homing to mucosal sites and is expressed on high endothelial venules (HEV) of both mesenteric lymph nodes and Peyer’s patches. It has also been found to be expressed on sinus-lining cells of the spleen. The integrin, alpha 4 beta 7, has been shown to function as the MAdCAM-1 receptor. The Ig domains of MAdCAM-1 have been found to be critical to alpha 4 beta 7 binding. The mucin domain has been shown to have activity in L-Selectin binding. MAdCAM-1 expression has been demonstrated to be up-regulated by TNF-alpha and IL-1 beta. MAdCAM-1 appears to play a role in inflammatory bowel disease (IBD) as its expression is highly up-regulated in IBD and most likely serves to recruit alpha 4 beta 7-expressing lymphocytes to the region. In vivo studies involving nonobese diabetic (NOD) mice have also suggested that MAdCAM-1/ alpha 4 beta 7 interaction plays a role in diabetes development in this model. Mouse MAdCAM-1 is a 405 amino acid (aa) residue protein with a 21 aa signal sequence, a 344 aa extracellular domain, a 20 aa transmembrane domain and a 20 aa cytoplasmic domain.

References
  1. Briskin, M.J. et al. (1993) Nature 363:461.
  2. Yang, X.D. et al. (1997) Diabetes 46:1542.
  3. Sampaio, S.O. et al. (1995) J. Immunol. 155:2477.
  4. Kraal, G. et al. (1995) Am. J. Pathol. 147:763.
  5. Berg, E.L. et al. (1993) Nature 366:695.
  6. Takeuchi, M. and V.R. Baichwal (1995) Proc. Natl. Acad. Sci. USA 92:3561.
Long Name
Mucosal Addressin Cell Adhesion Molecule 1
Entrez Gene IDs
8174 (Human); 17123 (Mouse)
Alternate Names
hMAdCAM-1; MACAM1; MAdCAM1; MAdCAM-1; mucosal addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule-1; mucosal vascular addressin cell adhesion molecule 1

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Citation for Mouse MAdCAM-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Targeting improves MSC treatment of inflammatory bowel disease.
    Authors: Ko IK, Kim BG, Awadallah A
    Mol. Ther., 2010-04-13;18(7):1365-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody

WB,ELISA

Reviews for Mouse MAdCAM-1 Antibody

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Mouse MAdCAM-1 Antibody
By Edward Sanderlin on 05/19/2016
Application: IHC Sample Tested: Colon tissue,Lymph node tissue Species: Mouse

Tissues fixed in 10% Formalin and embedded in paraffin blocks. Antigen retrieval in Tris-Base EDTA buffer pH 9.0. Incubated overnight with MAdCAM-1 at a concentration of 5ug/ml. Used CTS008 (Anti-Goat HRP-DAB cell and tissue staining kit) and saw signal in endothelial cells with no background.