Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Complex Species Category, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Electron Microscopy

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MMP-9 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MMP‑9
Ala20-Pro730
Accession # P41245

Specificity

Detects mouse MMP-9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) MMP-9 is observed and less than 2% cross-reactivity with rhMMP-1, -2, -3, -7, -8, -10, -12, and -13 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse MMP‑9 Antibody

Detection of Mouse MMP-9 antibody by Western Blot.

Detection of Mouse MMP‑9 by Western Blot.

Western blot shows lysates of mouse spleen tissue and mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for MMP-9 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MMP-9 antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

MMP‑9 in Mouse Splenocytes.

MMP-9 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Mouse MMP-9 antibody by Simple WesternTM.

Detection of Mouse MMP‑9 by Simple WesternTM.

Simple Western lane view shows lysates of mouse lung tissue and mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for MMP-9 at approximately 155 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

MMP‑9 antibody in Rat Brain by Immunohistochemistry (IHC-Fr).

MMP‑9 in Rat Brain.

MMP-9 was detected in perfusion fixed frozen sections of rat brain (cingulate cortex) using 1.7 µg/mL Goat Anti-Mouse MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse MMP-9 by Immunohistochemistry

Detection of Mouse MMP-9 by Immunohistochemistry

Remodeled ECM enhances the invasive behaviors of ovarian cancer cells. (a) H&E stain of the tumor lesions from WT and ApoE−/− mice at two weeks post ID8 engraftment (left). Graph represents the mean size of the lesions calculated from ten random fields (right). (b) Masson’s Trichrome stain of tumor lesions (left). The mean percentage of positive regions in ten random fields was calculated (right). (c) The cytokines/chemokines profile in the supernatants of ascites from WT and ApoE−/− mice. Four groups of mouse cytokine dot-blot arrays are shown. Dot-blots with significant changes are shown in boxed areas (red). (d) The top four pathways enriched among the molecules with significant changes using KEGG pathway analysis. (e) MMP-10 and MMP-9 protein expression in tumor lesions, determined by blinded IHC analysis (left). Box plot of the IHC score of MMP-10 and MMP-9 (right). Box represents the 25th–75th percentile while whiskers indicate the 5th–95th percentile. The black box represents tumor lesions from WT mice and the grey box represents tumor lesions from ApoE−/− mice. Each experiment included data from 5 mice. Bar represents 50 μm. *P < 0.05; **P < 0.005 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29458390), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse MMP-9 by Immunohistochemistry

Detection of Mouse MMP-9 by Immunohistochemistry

Remodeled ECM promotes malignancy of ovarian cancer via FAK-ERK-MMP activation. (a) Specimens from Fig. 5D were immunoassayed for p-FAKY397 (red), and nuclei were stained with Hoechst (blue). (b) IHC of active ERK (p-p44/42 MAPKThr202/Tyr204) in tumor lesions from WT and ApoE−/− mice with PBS or BAPN treatment two weeks after ID8 engraftment. (c) PBS or PD-325901 was administrated to ApoE−/− mice once ID8-Luciferase cells were intraperitoneally injected and treatment continued for one month. The representative images (top) and quantification data (bottom) of MMP-9 protein expression in tumor lesions two months after ID8 engraftment. (d) In vivo luciferase expression was determined two weeks or two months post treatment. Luminescence (right panel) is represented as the radiance (p/s/cm2/sr). Each experiment included data from 4 mice. Bar represents 50 μm. *P < 0.05; **P < 0.005 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29458390), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse MMP-9 by Western Blot

Detection of Mouse MMP-9 by Western Blot

Fibrotic deposit and cardiac dysfunction correlate with decreasing CVPC presence in mdx heart. (A) Representative images of histological analysis stained using Masson trichrome technique showing myocytes (in red) and collagenous fibrotic tissue (in blue) in the left ventricle of WT and mdx hearts at 9, 24, and 52 wo. Line represents 100 µm. (B) The ratio of red and blue stained tissue was evaluated in WT hearts (open bars and black dots, n = 4–11 slices/3 animals per group) and mdx hearts (black bars and grey dots, n = 3–16 slices/3 animals per group) at the age of 24 wo and further at 52 wo. Statistical significance was calculated by Kruskal–Wallis test and Dunn‘s multiple comparison post-hoc test (*** p < 0.001, **** p < 0.0001). (C) Western blot analysis of collagen proteins and inflammatory proteins in the cardiac tissues. Left panel shows representative images of collagen 1A1 (Coll 1A1), collagen 3 (Coll 3), cyclooxygenase 2 (COX-2), and matrix metalloproteinase 9 (MMP-9) compared to the GAPDH control. The right panels show the normalized densitometry of each protein normalized by GAPDH content of WT (open bars, n = 2 animals) and mdx (black bars, n = 2 animals). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34068508), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse MMP-9 by Immunocytochemistry/Immunofluorescence

Detection of Mouse MMP-9 by Immunocytochemistry/Immunofluorescence

MMP2, MMP9, and RANKL expression in AAAs.A. Immunohistochemical staining for indicated proteins of serial sections of aortas one week after CaCl2 treatment. Elastic van Gieson staining is also shown. SM alpha -actin and F4/80 were stained to locate SMCs and macrophages, respectively. Shown are representative images of 4 or more samples in each group. Scale bars, 50 µm. B. Relative positive staining area of MMP2, MMP9, and RANKL in sections from control diet and EPA diet groups. n = 4–5. *P<0.05. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0096286), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse MMP-9 by Immunohistochemistry

Detection of Mouse MMP-9 by Immunohistochemistry

BAPN treatment delays ovarian cancer progression by reducing adhesions. (a) Experimental design: PBS or BAPN was intraperitoneally administrated to 20-weeks-old female ApoE−/− mice each day and continued for four weeks. A cohort of mice was sacrificed for further experiments. For the remaining mice, the drug treatment was stopped for two weeks before the establishment of ID8 allografts. (b) Hydroxyproline was measured in the plasma and diaphragm. (c) Masson’s Trichrome stain after BAPN treatment (left). The positive-staining percentage of 10 random fields was calculated (right). Bar represents 50 μm. (d) Cells adhesive to the omentum were analyzed four hours after ID8 intraperitoneal injection by fluorescence microscopy (left). The adhesive cells were determined from the total fluorescent intensity after digestion (right). Bar represents 200 μm. (e) In vivo luciferase measured at two weeks (top) and two months (bottom) post establishment in ApoE−/− mice with PBS or BAPN pre-treatment. Quantification of luminescence is represented as the radiance. (f) MMP-9 expression measured by IHC in tumor lesions of ApoE−/− mice with PBS or BAPN treatment. Each experiment includes data from 4 mice. Bar represents 50 μm. *P < 0.05; **P < 0.005 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29458390), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of MMP‑9 in Mouse Thymus.

MMP‑9 was detected in immersion fixed paraffin-embedded sections of mouse thymus using Goat Anti-Mouse MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) at 1.7 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and macrophages. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Mouse MMP-9 by Flow Cytometry

Detection of Mouse MMP-9 by Flow Cytometry

Single cell RNA-sequencing analysis of healing bone fractures. A Preparation of non-haematopoietic stromal cells from PFD14 and control bone for scRNA-seq analysis. b UMAP plots showing colour-coded cell clusters from control and PFD14 bone. Cell types and numbers per cluster are displayed on the right. c–e Heatmap showing the top 5 marker genes for each cluster (c). Feature blots showing markers for non-haematopoietic bone cells: Acan—chondrocytes (CHO); Igfbp6—fibroblasts (FB); Pdgfrb—bone mesenchymal stromal cells (BMSCs); Col22a1—osteoblast lineage cells (OBs); Emcn—ECs; Rgs5—Smooth muscle cells (SMCs) (d). Comparison of marker gene expression in PFD14 and control samples (e). Haematopoietic cells (HCs-1,2,3,4). f, g UMAP plots showing colour-coded BMSC subclusters from control and PFD14 bone (f). Fabp5 and Mmp9 expressing cells are increased in PFD14 samples (g). h, i UMAP plot showing mpMSCs and SCs in the control and PFD14 mpMSC subcluster (h). Fracture-derived SCs show higher expression of Mmp9 and Fabp5 (i). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35091558), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse MMP-9 by Flow Cytometry

Detection of Mouse MMP-9 by Flow Cytometry

Single cell RNA-sequencing analysis of healing bone fractures. A Preparation of non-haematopoietic stromal cells from PFD14 and control bone for scRNA-seq analysis. b UMAP plots showing colour-coded cell clusters from control and PFD14 bone. Cell types and numbers per cluster are displayed on the right. c–e Heatmap showing the top 5 marker genes for each cluster (c). Feature blots showing markers for non-haematopoietic bone cells: Acan—chondrocytes (CHO); Igfbp6—fibroblasts (FB); Pdgfrb—bone mesenchymal stromal cells (BMSCs); Col22a1—osteoblast lineage cells (OBs); Emcn—ECs; Rgs5—Smooth muscle cells (SMCs) (d). Comparison of marker gene expression in PFD14 and control samples (e). Haematopoietic cells (HCs-1,2,3,4). f, g UMAP plots showing colour-coded BMSC subclusters from control and PFD14 bone (f). Fabp5 and Mmp9 expressing cells are increased in PFD14 samples (g). h, i UMAP plot showing mpMSCs and SCs in the control and PFD14 mpMSC subcluster (h). Fracture-derived SCs show higher expression of Mmp9 and Fabp5 (i). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35091558), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse MMP‑9 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse splenocytes

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (cingulate cortex), and Immersion fixed paraffin-embedded sections of mouse thymus

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse MMP‑9 (Catalog # 909-MM), see our available Western blot detection antibodies

Simple Western

5 µg/mL
Sample: Mouse lung tissue and mouse spleen tissue

Western Blot

0.25 µg/mL
Sample: Mouse spleen tissue and mouse lung tissue

Reviewed Applications

Read 4 reviews rated 4.5 using AF909 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-9

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin, and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages, and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 may be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain. Compared to the human MMP-9
(Catalog # http://www.rndsystems.com/product_results.aspx?k=911-MP">911-MP), the mouse enzyme contains extra sequences in the linker region and in the hemopexin-like domain, respectively.

Long Name

Matrix Metalloproteinase 9

Alternate Names

CLG4B, Gelatinase B, GELB, MANDP2, MMP9

Entrez Gene IDs

4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)

Gene Symbol

MMP9

UniProt

P41245

Additional MMP-9 Products

Product Documents for Mouse MMP‑9 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse MMP‑9 Antibody

For research use only

Citations for Mouse MMP‑9 Antibody

Customer Reviews for Mouse MMP‑9 Antibody (4)

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Mouse MMP-9 Antibody
    Name: Xiaoming Zhou
    Application: Western Blot
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 10/27/2017
    we tried other mmp9 antibody for mouse, this one works best of all!
    Mouse MMP‑9 Antibody AF909
  • Mouse MMP-9 Antibody
    Name: Julie Van
    Application: Western Blot
    Sample Tested: Kidney tissue
    Species: Mouse
    Verified Customer | Posted 09/01/2017
    Mouse MMP‑9 Antibody AF909
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23766088
    Species: Mouse
    Verified Customer | Posted 01/12/2015
  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: See PMID 23060524
    Species: Mouse
    Verified Customer | Posted 01/12/2015

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Protocols

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FAQs for Mouse MMP‑9 Antibody

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  • Q: Does Mouse MMP-9 antibody, catalog # AF909, detect the pro or active form of MMP-9? 

    A: This antibody can detect both the pro and active form of mouse MMP-9. 

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