Mouse MMP-9 Antibody

(46 citations)
(2 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse MMP-9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) MMP-9 is observed and less than 2% cross-reactivity with rhMMP-1, -2, -3, -7, -8, -10, -12, and -13 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse MMP‑9
    Ala20-Pro730
    Accession # P41245
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    .25 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Mouse MMP‑9 (Catalog # 909-MM), see our available Western blot detection antibodies
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse MMP‑9 by Western Blot. Western blot shows lysates of mouse spleen tissue and mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MMP‑9 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
MMP‑9 in Rat Brain. MMP‑9 was detected in perfusion fixed frozen sections of rat brain (cingulate cortex) using 1.7 µg/mL Goat Anti-Mouse MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse MMP‑9 by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue and mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for MMP‑9 at approximately 155 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse MMP‑9 Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF909) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-9
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin, and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages, and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 may be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain. Compared to the human MMP-9
(Catalog # 911-MP), the mouse enzyme contains extra sequences in the linker region and in the hemopexin-like domain, respectively.
  • Long Name:
    Matrix Metalloproteinase 9
  • Entrez Gene IDs:
    4318 (Human); 17395 (Mouse); 81687 (Rat)
  • Alternate Names:
    92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

46 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Immunodominant fragments of myelin basic protein initiate T cell-dependent pain.
    J Neuroinflammation, 2102;9(0):119.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. TIMP-1 is upregulated, but not essential in hepatic fibrogenesis and carcinogenesis in mice
    Authors: ND Thiele, JW Wirth, D Steins, AC Koop, H Ittrich, AW Lohse, J Kluwe
    Sci Rep, 2017;7(1):714.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Endotoxemia shifts neutrophils with TIMP-free gelatinase B/ MMP-9 from bone marrow to periphery and induces systematic upregulation of TIMP-1
    Authors: J Vandooren, W Swinnen, E Ugarte-Ber, L Boon, D Dorst, E Martens, G Opdenakker
    Haematologica, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC paraffin embedded
  4. Smooth muscle cell-specific Tgfbr1 deficiency attenuates neointimal hyperplasia but promotes an undesired vascular phenotype for injured arteries
    Physiol Rep, 2016;4(23):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Bexarotene targets autophagy and is protective against thromboembolic stroke in aged mice with tauopathy
    Sci Rep, 2016;6(0):33176.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  6. Thymocyte development in the absence of matrix metalloproteinase-9/gelatinase B
    Sci Rep, 2016;6(0):29852.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. Possible dual role of decorin in abdominal aortic aneurysm.
    Authors: Ueda, Koshiro, Yoshimura, Koichi, Yamashita, Osamu, Harada, Takasuke, Morikage, Noriyasu, Hamano, Kimikazu
    PLoS ONE, 2015;10(3):e0120689.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  8. Matrix Metalloproteinase-2 (MMP-2) Gene Deletion Enhances MMP-9 Activity, Impairs PARP-1 Degradation, and Exacerbates Hepatic Ischemia and Reperfusion Injury in Mice.
    Authors: Kato H, Duarte S, Liu D, Busuttil R, Coito A
    PLoS ONE, 2015;10(9):e0137642.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  9. Cytomegalovirus initiates infection selectively from high-level beta1 integrin-expressing cells in the brain.
    Authors: Kawasaki H, Kosugi I, Sakao-Suzuki M, Meguro S, Arai Y, Tsutsui Y, Iwashita T
    Am J Pathol, 2015;185(5):1304-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: WB
  10. MMP-9 deficiency shelters endothelial PECAM-1 expression and enhances regeneration of steatotic livers after ischemia and reperfusion injury.
    Authors: Kato H, Kuriyama N, Duarte S, Clavien P, Busuttil R, Coito A
    J Hepatol, 2014;60(5):1032-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  11. The mineral dissolution function of osteoclasts is dispensable for hypertrophic cartilage degradation during long bone development and growth.
    Authors: Touaitahuata H, Cres G, de Rossi S, Vives V, Blangy A
    Dev Biol, 2014;393(1):57-70.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  12. Molecular hydrogen in drinking water protects against neurodegenerative changes induced by traumatic brain injury.
    Authors: Dohi, Kenji, Kraemer, Brian C, Erickson, Michelle, McMillan, Pamela J, Kovac, Andrej, Flachbartova, Zuzana, Hansen, Kim M, Shah, Gul N, Sheibani, Nader, Salameh, Therese, Banks, William
    PLoS ONE, 2014;9(9):e108034.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  13. Platelets guide the formation of early metastatic niches.
    Authors: Labelle, Myriam, Begum, Shahinoo, Hynes, Richard
    Proc Natl Acad Sci U S A, 2014;111(30):E3053-61.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  14. Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity.
    Authors: Park D, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong K, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong Y, Koh G
    J Clin Invest, 2014;124(9):3960-74.
  15. The omega-3 polyunsaturated fatty acid, eicosapentaenoic acid, attenuates abdominal aortic aneurysm development via suppression of tissue remodeling.
    Authors: Wang J, Eguchi K, Matsumoto S, Fujiu K, Komuro I, Nagai R, Manabe I
    PLoS ONE, 2014;9(5):e96286.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  16. Hepatic ischemia and reperfusion injury in the absence of myeloid cell-derived COX-2 in mice.
    Authors: Duarte, Sergio, Kato, Hiroyuki, Kuriyama, Naohisa, Suko, Kathryn, Ishikawa, Tomo-O, Busuttil, Ronald W, Herschman, Harvey R, Coito, Ana J
    PLoS ONE, 2014;9(5):e96913.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  17. Chemerin neutralization blocks hematopoietic stem cell osteoclastogenesis.
    Authors: Muruganandan S, Dranse H, Rourke J, McMullen N, Sinal C
    Stem Cells, 2013;31(10):2172-82.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  18. Thrombospondin-1 induction in the diabetic myocardium stabilizes the cardiac matrix in addition to promoting vascular rarefaction through angiopoietin-2 upregulation.
    Authors: Gonzalez-Quesada C, Cavalera M, Biernacka A, Kong P, Lee D, Saxena A, Frunza O, Dobaczewski M, Shinde A, Frangogiannis N
    Circ Res, 2013;113(12):1331-44.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  19. CD43-mediated IFN-gamma production by CD8+ T cells promotes abdominal aortic aneurysm in mice.
    Authors: Zhou H, Yan H, Cannon J, Springer L, Green J, Pham C
    J Immunol, 2013;190(10):5078-85.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  20. KLF4 regulates abdominal aortic aneurysm morphology and deletion attenuates aneurysm formation.
    Authors: Salmon M, Johnston W, Woo A, Pope N, Su G, Upchurch G, Owens G, Ailawadi G
    Circulation, 2013;128(11):S163-74.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  21. The MMP-9/TIMP-1 axis controls the status of differentiation and function of myelin-forming Schwann cells in nerve regeneration.
    Authors: Kim Y, Remacle AG, Chernov AV
    PLoS ONE, 2012;7(3):e33664.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  22. Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice.
    Authors: Xue M, Dervish S, Harrison LC, Fulcher G, Jackson CJ
    J. Biol. Chem., 2012;287(20):16356-64.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  23. Tissue inhibitor of matrix metalloproteinase 1 (TIMP1) controls adipogenesis in obesity in mice and in humans.
    Authors: Meissburger B, Stachorski L, Roder E, Rudofsky G, Wolfrum C
    Diabetologia, 2011;54(6):1468-79.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  24. Osteopontin-Stimulated Expression of Matrix Metalloproteinase-9 Causes Cardiomyopathy in the mdx Model of Duchenne Muscular Dystrophy.
    Authors: Dahiya S, Givvimani S, Bhatnagar S
    J. Immunol., 2011;187(5):2723-31.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  25. Tumor necrosis factor-alpha regulates distinct molecular pathways and gene networks in cultured skeletal muscle cells.
    Authors: Bhatnagar S, Panguluri SK, Gupta SK, Dahiya S, Lundy RF, Kumar A
    PLoS ONE, 2010;5(10):e13262.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  26. Matrix metalloproteinase-9 regulates tumor cell invasion through cleavage of protease nexin-1.
    Authors: Xu D, McKee CM, Cao Y
    Cancer Res., 2010;70(17):6988-98.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  27. TRE17/USP6 oncogene translocated in aneurysmal bone cyst induces matrix metalloproteinase production via activation of NF-kappaB.
    Authors: Ye Y, Pringle LM, Lau AW
    Oncogene, 2010;29(25):3619-29.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  28. Adenosine and osteopontin contribute to the development of chronic obstructive pulmonary disease.
    Authors: Schneider DJ, Lindsay JC, Zhou Y, Molina JG, Blackburn MR
    FASEB J., 2010;24(1):70-80.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  29. Matrix metalloproteinase-9 deficiency worsens lung injury in a model of bronchopulmonary dysplasia.
    Authors: Lukkarinen H, Hogmalm A, Lappalainen U, Bry K
    Am. J. Respir. Cell Mol. Biol., 2009;41(1):59-68.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  30. Molecular role of Cx37 in advanced atherosclerosis: a micro-array study.
    Authors: Derouette JP, Wong C, Burnier L, Morel S, Sutter E, Galan K, Brisset AC, Roth I, Chadjichristos CE, Kwak BR
    Atherosclerosis, 2009;206(1):69-76.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  31. A TLR2 agonist in German cockroach frass activates MMP-9 release and is protective against allergic inflammation in mice.
    Authors: Page K, Ledford JR, Zhou P, Wills-Karp M
    J. Immunol., 2009;183(5):3400-8.
    Species: Mouse
    Sample Type: BALF
    Application: WB
  32. Inducible nitric oxide synthase deficiency impairs matrix metalloproteinase-9 activity and disrupts leukocyte migration in hepatic ischemia/reperfusion injury.
    Authors: Hamada T, Duarte S, Tsuchihashi S, Busuttil RW, Coito AJ
    Am. J. Pathol., 2009;174(6):2265-77.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  33. Cyclooxygenase-2 deficiency enhances Th2 immune responses and impairs neutrophil recruitment in hepatic ischemia/reperfusion injury.
    Authors: Hamada T, Tsuchihashi S, Avanesyan A, Duarte S, Moore C, Busuttil RW, Coito AJ
    J. Immunol., 2008;180(3):1843-53.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  34. Increased gelatinase B/matrix metalloproteinase 9 (MMP-9) activity in a murine model of acute coxsackievirus B4-induced pancreatitis.
    Authors: De Palma AM, Verbeken E, Van Aelst I, Van den Steen PE, Opdenakker G, Neyts J
    Virology, 2008;382(1):20-7.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  35. Genetic deficiency of heme oxygenase-1 impairs functionality and form of an arteriovenous fistula in the mouse.
    Authors: Juncos JP, Tracz MJ, Croatt AJ, Grande JP, Ackerman AW, Katusic ZS, Nath KA
    Kidney Int., 2008;74(1):47-51.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  36. A novel method to establish microglia-free astrocyte cultures: comparison of matrix metalloproteinase expression profiles in pure cultures of astrocytes and microglia.
    Authors: Crocker SJ, Frausto RF, Whitton JL, Milner R
    Glia, 2008;56(0):1187-98.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  37. A3 adenosine receptor signaling influences pulmonary inflammation and fibrosis.
    Authors: Morschl E, Molina JG, Volmer JB, Mohsenin A, Pero RS, Hong JS, Kheradmand F, Lee JJ, Blackburn MR
    Am. J. Respir. Cell Mol. Biol., 2008;39(6):697-705.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  38. Functional relevance of the IL-23-IL-17 axis in lungs in vivo.
    Authors: Ivanov S, Bozinovski S, Bossios A, Valadi H, Vlahos R, Malmhall C, Sjostrand M, Kolls JK, Anderson GP, Linden A
    Am. J. Respir. Cell Mol. Biol., 2007;36(4):442-51.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  39. Dendritic cell transmigration through brain microvessel endothelium is regulated by MIP-1alpha chemokine and matrix metalloproteinases.
    Authors: Zozulya AL, Reinke E, Baiu DC, Karman J, Sandor M, Fabry Z
    J. Immunol., 2007;178(1):520-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  40. Angiostatin inhibits monocyte/macrophage migration via disruption of actin cytoskeleton.
    Authors: Perri SR, Annabi B, Galipeau J
    FASEB J., 2007;21(14):3928-36.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  41. A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex.
    Authors: Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J
    Blood, 2007;109(5):2234-42.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  42. Matrix metalloproteinase-2 (MMP-2) regulates astrocyte motility in connection with the actin cytoskeleton and integrins.
    Authors: Ogier C, Bernard A, Chollet AM, LE Diguardher T, Hanessian S, Charton G, Khrestchatisky M, Rivera S
    Glia, 2006;54(4):272-84.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  43. Interleukin-17 as a recruitment and survival factor for airway macrophages in allergic airway inflammation.
    Authors: Sergejeva S, Ivanov S, Lotvall J, Linden A
    Am. J. Respir. Cell Mol. Biol., 2005;33(3):248-53.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  44. Expression of matrix metalloproteinases subsequent to urogenital Chlamydia muridarum infection of mice.
    Authors: Ramsey KH, Sigar IM, Schripsema JH, Shaba N, Cohoon KP
    Infect. Immun., 2005;73(10):6962-73.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  45. Upregulation of MMP-9/TIMP-1 enzymatic system in eosinophilic meningitis caused by Angiostrongylus cantonensis.
    Authors: Chen KM, Lee HH, Chou HL, Liu JY, Tsai BC, Lai SC
    Int J Exp Pathol, 2005;86(2):81-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  46. Ultrastructural localization of matrix metalloproteinase-9 in eosinophils from the cerebrospinal fluid of mice with eosinophilic meningitis caused by Angiostrongylus cantonensis.
    Authors: Tseng YK, Tu WC, Lee HH, Chen KM, Chou HL, Lai SC
    Ann Trop Med Parasitol, 2004;98(8):831-41.
    Species: Mouse
    Sample Type: CSF
    Application: Electron Microscopy
Expand to show all 46 Citations
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Average Rating: 5 (Based on 2 reviews)

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Western Blot Mouse MMP-9 Antibody AF909
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 WB Mouse Anonymous 10/27/2017
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Western Blot Mouse MMP-9 Antibody AF909
Western Blot: Mouse MMP-9 Antibody [AF909]

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ApplicationWestern Blot
Sample TestedLung tissue
SpeciesMouse

Other Experimental Details

Other Experimental Detailswe tried other mmp9 antibody for mouse, this one works best of all!
Western Blot Mouse MMP-9 Antibody AF909
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Excellent
 WB Mouse Anonymous 09/01/2017
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Western Blot Mouse MMP-9 Antibody AF909
Western Blot: Mouse MMP-9 Antibody [AF909]

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ApplicationWestern Blot
Sample TestedKidney tissue
SpeciesMouse

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